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miR-198-induced upregulation of Livin may be associated with the prognosis and contribute to the oncogenesis of lung adenocarcinoma

  • Authors:
    • Yicheng Liang
    • Hetan Wang
    • Yuanyuan Sun
    • Sheng Chen
    • Haoyou Wang
    • Rong Huang
    • Xinyi Zhao
    • Weineng Fu
    • Chunlu Yang
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Medical Genetics, China Medical University, Shenyang, Liaoning 110001, P.R. China, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China
    Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2096-2104
    |
    Published online on: August 1, 2017
       https://doi.org/10.3892/or.2017.5866
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Abstract

Livin, a member of the inhibitor of apoptosis protein (IAP) family, is expressed at a high level in lung adenocarcinoma and influences the progression of cancer, and its response to chemotherapy and radiotherapy. Aberrant microRNA (miRNA) expression has also been associated with cancer initiation and development. However, the clinical significance of Livin and its relationship with miRNAs in lung adenocarcinoma are still unclear. In the present study, the expression level of Livin in 90 pairs of lung adenocarcinoma and their adjacent tissues were detected by immunohistochemistry staining. Spearman correlation and Kaplan-Meier, univariate and multivariate analyses were applied to evaluate the correlation between the expression of Livin and clinical characteristics. With the integration of bioinformatics analysis and dual-luciferase reporter gene assays, we identified the miRNA that can target Livin mRNA. The functional effects of miRNA-mediated Livin knockdown were assessed by Cell Counting Kit-8 (CCK-8) and apoptosis assays, and cell cycle analysis. The present study revealed that Livin was upregulated in lung adenocarcinoma tissues and may be associated with the poor prognosis in lung adenocarcinoma patients. The overexpression of Livin is partly caused by the downregulation of miR-198. Further exploration revealed that miRNA-198-mediated silencing of Livin significantly inhibited cell growth and enhanced apoptosis of A549 cells, accompanied by marked upregulation of caspase-3. Finally, we observed that the miR-198 overexpression and Livin neutralization had similar effects on improving cisplatin chemosensitivity in A549 cells. Overall, these findings suggest that Livin has the potential to become a biomarker for predicting the prognosis of lung adenocarcinoma and may provide a promising strategy for assisting chemotherapy of lung adenocarcinoma through the miR-198/Livin/caspase-3 regulatory network.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Diaz-Garcia CV, Agudo-Lopez A, Perez C, López-Martín JA, Rodríguez-Peralto JL, de Castro J, Cortijo A, Martínez-Villanueva M, Iglesias L, García-Carbonero R, et al: DICER1, DROSHA and miRNAs in patients with non-small cell lung cancer: Implications for outcomes and histologic classification. Carcinogenesis. 34:1031–1038. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Hariu H, Hirohashi Y, Torigoe T, Asanuma H, Hariu M, Tamura Y, Aketa K, Nabeta C, Nakanishi K, Kamiguchi K, et al: Aberrant expression and potency as a cancer immunotherapy target of inhibitor of apoptosis protein family, Livin/ML-IAP in lung cancer. Clin Cancer Res. 11:1000–1009. 2005.PubMed/NCBI

4 

Li J, Chen P, Li XQ, Bao QL, Dai CH and Ge LP: Elevated levels of survivin and livin mRNA in bronchial aspirates as markers to support the diagnosis of lung cancer. Int J Cancer. 132:1098–1104. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Tanabe H, Yagihashi A, Tsuji N, Shijubo Y, Abe S and Watanabe N: Expression of survivin mRNA and livin mRNA in non-small-cell lung cancer. Lung Cancer. 46:299–304. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Zhuang L, Shen LD, Li K, Yang RX, Zhang QY, Chen Y, Gao CL, Dong C, Bi Q, Tao JN, et al: Inhibition of livin expression suppresses cell proliferation and enhances chemosensitivity to cisplatin in human lung adenocarcinoma cells. Mol Med Rep. 12:547–552. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Yuan D, Liu L, Xu H and Gu D: The effects on cell growth and chemosensitivity by livin RNAi in non-small cell lung cancer. Mol Cell Biochem. 320:133–140. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Sun JG, Liao RX, Zhang SX, Duan YZ, Zhuo WL, Wang XX, Wang ZX, Li DZ and Chen ZT: Role of inhibitor of apoptosis protein Livin in radiation resistance in nonsmall cell lung cancer. Cancer Biother Radiopharm. 26:585–592. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Kasof GM and Gomes BC: Livin, a novel inhibitor of apoptosis protein family member. J Biol Chem. 276:3238–3246. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Crnkovic-Mertens I, Muley T, Meister M, Hartenstein B, Semzow J, Butz K and Hoppe-Seyler F: The anti-apoptotic livin gene is an important determinant for the apoptotic resistance of non-small cell lung cancer cells. Lung Cancer. 54:135–142. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Lin X, Li HR, Lin XF, Yu ME, Tu XW, Hua ZD, Lin M, Xu NL, Han LL and Chen YS: Silencing of Livin inhibits tumorigenesis and metastasis via VEGF and MMPs pathway in lung cancer. Int J Oncol. 47:657–667. 2015.PubMed/NCBI

12 

Lau NC, Lim LP, Weinstein EG and Bartel DP: An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science. 294:858–862. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Lee RC and Ambros V: An extensive class of small RNAs in Caenorhabditis elegans. Science. 294:862–864. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Kim VN and Nam JW: Genomics of microRNA. Trends in genetics. TIG. 22:165–173. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Farazi TA, Spitzer JI, Morozov P and Tuschl T: miRNAs in human cancer. J Pathol. 223:102–115. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Puisségur MP, Mazure NM, Bertero T, Pradelli L, Grosso S, Robbe-Sermesant K, Maurin T, Lebrigand K, Cardinaud B, Hofman V, et al: miR-210 is overexpressed in late stages of lung cancer and mediates mitochondrial alterations associated with modulation of HIF-1 activity. Cell Death Differ. 18:465–478. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Wu S, Zhang G, Li P, Chen S, Zhang F, Li J, Jiang C, Chen X, Wang Y, Du Y, et al: miR-198 targets SHMT1 to inhibit cell proliferation and enhance cell apoptosis in lung adenocarcinoma. Tumour Biol. 37:5193–5202. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Pardo OE, Castellano L, Munro CE, Hu Y, Mauri F, Krell J, Lara R, Pinho FG, Choudhury T, Frampton AE, et al: miR-515-5p controls cancer cell migration through MARK4 regulation. EMBO Rep. 17:570–584. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Chen YS, Li HR, Lin M, Chen G, Xie BS, Xu NL and Lin LF: Livin abrogates apoptosis of SPC-A1 cell by regulating JNKI signaling pathway. Mol Biol Rep. 37:2241–2247. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Dubrez-Daloz L, Dupoux A and Cartier J: IAPs: More than just inhibitors of apoptosis proteins. Cell Cycle. 7:1036–1046. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Sun J, Liao R, Chen Z, Wang Z, Zhang Q and Hu Y: Study on enhancing sensitivity of SPC-A1 cells to chemotherapy by Livin isoform-specific gene silencing. Zhongguo Fei Ai Za Zhi. 10:461–465. 2007.(In Chinese). PubMed/NCBI

22 

Yuan D, Liu L and Gu D: Transcriptional regulation of livin by beta-catenin/TCF signaling in human lung cancer cell lines. Mol Cell Biochem. 306:171–178. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Yang J, Zhao H, Xin Y and Fan L: MicroRNA-198 inhibits proliferation and induces apoptosis of lung cancer cells via targeting FGFR1. J Cell Biochem. 115:987–995. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Gigante M, Pontrelli P, Herr W, Gigante M, D'Avenia M, Zaza G, Cavalcanti E, Accetturo M, Lucarelli G, Carrieri G, et al: miR-29b and miR-198 overexpression in CD8+ T cells of renal cell carcinoma patients down-modulates JAK3 and MCL-1 leading to immune dysfunction. J Transl Med. 14:842016. View Article : Google Scholar : PubMed/NCBI

25 

Zhang S, Zhao Y and Wang L: MicroRNA-198 inhibited tumorous behaviors of human osteosarcoma through directly targeting ROCK1. Biochem Biophys Res Commun. 472:557–565. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Wang M, Wang J, Kong X, Chen H, Wang Y, Qin M, Lin Y, Chen H, Xu J, Hong J, et al: MiR-198 represses tumor growth and metastasis in colorectal cancer by targeting fucosyl transferase 8. Sci Rep. 4:61452014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liang Y, Wang H, Sun Y, Chen S, Wang H, Huang R, Zhao X, Fu W and Yang C: miR-198-induced upregulation of Livin may be associated with the prognosis and contribute to the oncogenesis of lung adenocarcinoma. Oncol Rep 38: 2096-2104, 2017.
APA
Liang, Y., Wang, H., Sun, Y., Chen, S., Wang, H., Huang, R. ... Yang, C. (2017). miR-198-induced upregulation of Livin may be associated with the prognosis and contribute to the oncogenesis of lung adenocarcinoma. Oncology Reports, 38, 2096-2104. https://doi.org/10.3892/or.2017.5866
MLA
Liang, Y., Wang, H., Sun, Y., Chen, S., Wang, H., Huang, R., Zhao, X., Fu, W., Yang, C."miR-198-induced upregulation of Livin may be associated with the prognosis and contribute to the oncogenesis of lung adenocarcinoma". Oncology Reports 38.4 (2017): 2096-2104.
Chicago
Liang, Y., Wang, H., Sun, Y., Chen, S., Wang, H., Huang, R., Zhao, X., Fu, W., Yang, C."miR-198-induced upregulation of Livin may be associated with the prognosis and contribute to the oncogenesis of lung adenocarcinoma". Oncology Reports 38, no. 4 (2017): 2096-2104. https://doi.org/10.3892/or.2017.5866
Copy and paste a formatted citation
x
Spandidos Publications style
Liang Y, Wang H, Sun Y, Chen S, Wang H, Huang R, Zhao X, Fu W and Yang C: miR-198-induced upregulation of Livin may be associated with the prognosis and contribute to the oncogenesis of lung adenocarcinoma. Oncol Rep 38: 2096-2104, 2017.
APA
Liang, Y., Wang, H., Sun, Y., Chen, S., Wang, H., Huang, R. ... Yang, C. (2017). miR-198-induced upregulation of Livin may be associated with the prognosis and contribute to the oncogenesis of lung adenocarcinoma. Oncology Reports, 38, 2096-2104. https://doi.org/10.3892/or.2017.5866
MLA
Liang, Y., Wang, H., Sun, Y., Chen, S., Wang, H., Huang, R., Zhao, X., Fu, W., Yang, C."miR-198-induced upregulation of Livin may be associated with the prognosis and contribute to the oncogenesis of lung adenocarcinoma". Oncology Reports 38.4 (2017): 2096-2104.
Chicago
Liang, Y., Wang, H., Sun, Y., Chen, S., Wang, H., Huang, R., Zhao, X., Fu, W., Yang, C."miR-198-induced upregulation of Livin may be associated with the prognosis and contribute to the oncogenesis of lung adenocarcinoma". Oncology Reports 38, no. 4 (2017): 2096-2104. https://doi.org/10.3892/or.2017.5866
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