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Downregulation of BZW2 inhibits osteosarcoma cell growth by inactivating the Akt/mTOR signaling pathway

  • Authors:
    • Dong-Dong Cheng
    • Shi-Jie Li
    • Bin Zhu
    • Ting Yuan
    • Qing-Cheng Yang
    • Cun-Yi Fan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
    Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2116-2122
    |
    Published online on: August 8, 2017
       https://doi.org/10.3892/or.2017.5890
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Abstract

Osteosarcoma is the most common malignant bone tumor in adolescents. The function of basic leucine zipper and W2 domains 2 (BZW2) in tumor progression has been reported. However, the role and mechanisms of BZW2 in osteosarcoma remain to be determined. The aim of the present study was to reveal the expression and biological functions of BZW2 in osteosarcoma and to elucidate the proximal mechanisms underlying these functions. The expression of BZW2 in osteosarcoma tissues and cell lines was assessed by qRT-PCR, western blotting and immunohistochemistry. BZW2 overexpression was detected in osteosarcoma cell lines. Clinically, BZW2 expression was higher in osteosarcoma tissues than in corresponding non-tumor tissues and was associated with advanced Enneking stage and tumor recurrence. The knockdown of BZW2 using siRNA inhibited osteosarcoma cell proliferation, colony-forming ability, and the cell cycle at the G2/M phase in vitro. Host signaling pathways affected by BZW2 were detected using a PathScan Intracellular Signaling Antibody Array kit. These data demonstrated that the knockdown of BZW2 suppresses protein phosphorylation in the Akt/mTOR signaling pathway. These observations suggest that BZW2 is upregulated and has a pro-tumor effect in osteosarcoma via activation of the Akt/mTOR signaling pathway and thus is a potential target for gene therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng D, Li S, Zhu B, Yuan T, Yang Q and Fan C: Downregulation of BZW2 inhibits osteosarcoma cell growth by inactivating the Akt/mTOR signaling pathway. Oncol Rep 38: 2116-2122, 2017.
APA
Cheng, D., Li, S., Zhu, B., Yuan, T., Yang, Q., & Fan, C. (2017). Downregulation of BZW2 inhibits osteosarcoma cell growth by inactivating the Akt/mTOR signaling pathway. Oncology Reports, 38, 2116-2122. https://doi.org/10.3892/or.2017.5890
MLA
Cheng, D., Li, S., Zhu, B., Yuan, T., Yang, Q., Fan, C."Downregulation of BZW2 inhibits osteosarcoma cell growth by inactivating the Akt/mTOR signaling pathway". Oncology Reports 38.4 (2017): 2116-2122.
Chicago
Cheng, D., Li, S., Zhu, B., Yuan, T., Yang, Q., Fan, C."Downregulation of BZW2 inhibits osteosarcoma cell growth by inactivating the Akt/mTOR signaling pathway". Oncology Reports 38, no. 4 (2017): 2116-2122. https://doi.org/10.3892/or.2017.5890
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng D, Li S, Zhu B, Yuan T, Yang Q and Fan C: Downregulation of BZW2 inhibits osteosarcoma cell growth by inactivating the Akt/mTOR signaling pathway. Oncol Rep 38: 2116-2122, 2017.
APA
Cheng, D., Li, S., Zhu, B., Yuan, T., Yang, Q., & Fan, C. (2017). Downregulation of BZW2 inhibits osteosarcoma cell growth by inactivating the Akt/mTOR signaling pathway. Oncology Reports, 38, 2116-2122. https://doi.org/10.3892/or.2017.5890
MLA
Cheng, D., Li, S., Zhu, B., Yuan, T., Yang, Q., Fan, C."Downregulation of BZW2 inhibits osteosarcoma cell growth by inactivating the Akt/mTOR signaling pathway". Oncology Reports 38.4 (2017): 2116-2122.
Chicago
Cheng, D., Li, S., Zhu, B., Yuan, T., Yang, Q., Fan, C."Downregulation of BZW2 inhibits osteosarcoma cell growth by inactivating the Akt/mTOR signaling pathway". Oncology Reports 38, no. 4 (2017): 2116-2122. https://doi.org/10.3892/or.2017.5890
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