Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells

  • Authors:
    • Wei Wu
    • Jingdi Chen
    • Jicheng Wu
    • Jun Lin
    • Sheng Yang
    • Honggang Yu
  • View Affiliations

  • Published online on: August 10, 2017     https://doi.org/10.3892/or.2017.5896
  • Pages: 2480-2488
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Abstract

The aim of the present study was to clarify the clinical implication and functional role of tripartite motif-59 (TRIM59) in colorectal carcinoma (CRC) and explore the underlying mechanism of aberrant high expression of TRIM59 in cancer. We validated that TRIM59 was upregulated in CRC samples, and also demonstrated that its upregulation was associated with advanced tumor stage of CRC patients; and its high expression indicated shorter overall survival and faster recurrence. Knockdown of TRIM59 significantly inhibited cell proliferation, migration and invasion. Cell cycle analysis showed that TRIM59-depleted cells accumulated in S-phase. In addition, the cell cycle regulators CDC25C, cyclin B1 and cyclin D1 were decreased by TRIM59 siRNA mediated knockdown. Furthermore, the depletion of TRIM59 promoted apoptosis in cell culture as indicated by the cleavage of caspase-3 and PARP when TRIM59 was depleted. These results suggested that TRIM59 is upregulated in human colorectal tumors compared with non-tumor tissues. The level of TRIM59 is correlated with malignant features of CRC and may serve as potential therapeutic and preventive strategies for CRC.
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October-2017
Volume 38 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wu W, Chen J, Wu J, Lin J, Yang S and Yu H: Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells. Oncol Rep 38: 2480-2488, 2017
APA
Wu, W., Chen, J., Wu, J., Lin, J., Yang, S., & Yu, H. (2017). Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells. Oncology Reports, 38, 2480-2488. https://doi.org/10.3892/or.2017.5896
MLA
Wu, W., Chen, J., Wu, J., Lin, J., Yang, S., Yu, H."Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells". Oncology Reports 38.4 (2017): 2480-2488.
Chicago
Wu, W., Chen, J., Wu, J., Lin, J., Yang, S., Yu, H."Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells". Oncology Reports 38, no. 4 (2017): 2480-2488. https://doi.org/10.3892/or.2017.5896