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Article

Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells

  • Authors:
    • Wei Wu
    • Jingdi Chen
    • Jicheng Wu
    • Jun Lin
    • Sheng Yang
    • Honggang Yu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, 73th Contingent, 95969 Troops, The Airborne Force of Chinese PLA, Wuhan, Hubei 430300, P.R. China, Tumor Basic and Translational Laboratory, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
  • Pages: 2480-2488
    |
    Published online on: August 10, 2017
       https://doi.org/10.3892/or.2017.5896
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Abstract

The aim of the present study was to clarify the clinical implication and functional role of tripartite motif-59 (TRIM59) in colorectal carcinoma (CRC) and explore the underlying mechanism of aberrant high expression of TRIM59 in cancer. We validated that TRIM59 was upregulated in CRC samples, and also demonstrated that its upregulation was associated with advanced tumor stage of CRC patients; and its high expression indicated shorter overall survival and faster recurrence. Knockdown of TRIM59 significantly inhibited cell proliferation, migration and invasion. Cell cycle analysis showed that TRIM59-depleted cells accumulated in S-phase. In addition, the cell cycle regulators CDC25C, cyclin B1 and cyclin D1 were decreased by TRIM59 siRNA mediated knockdown. Furthermore, the depletion of TRIM59 promoted apoptosis in cell culture as indicated by the cleavage of caspase-3 and PARP when TRIM59 was depleted. These results suggested that TRIM59 is upregulated in human colorectal tumors compared with non-tumor tissues. The level of TRIM59 is correlated with malignant features of CRC and may serve as potential therapeutic and preventive strategies for CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Wu W, Chen J, Wu J, Lin J, Yang S and Yu H: Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells. Oncol Rep 38: 2480-2488, 2017.
APA
Wu, W., Chen, J., Wu, J., Lin, J., Yang, S., & Yu, H. (2017). Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells. Oncology Reports, 38, 2480-2488. https://doi.org/10.3892/or.2017.5896
MLA
Wu, W., Chen, J., Wu, J., Lin, J., Yang, S., Yu, H."Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells". Oncology Reports 38.4 (2017): 2480-2488.
Chicago
Wu, W., Chen, J., Wu, J., Lin, J., Yang, S., Yu, H."Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells". Oncology Reports 38, no. 4 (2017): 2480-2488. https://doi.org/10.3892/or.2017.5896
Copy and paste a formatted citation
x
Spandidos Publications style
Wu W, Chen J, Wu J, Lin J, Yang S and Yu H: Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells. Oncol Rep 38: 2480-2488, 2017.
APA
Wu, W., Chen, J., Wu, J., Lin, J., Yang, S., & Yu, H. (2017). Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells. Oncology Reports, 38, 2480-2488. https://doi.org/10.3892/or.2017.5896
MLA
Wu, W., Chen, J., Wu, J., Lin, J., Yang, S., Yu, H."Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells". Oncology Reports 38.4 (2017): 2480-2488.
Chicago
Wu, W., Chen, J., Wu, J., Lin, J., Yang, S., Yu, H."Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells". Oncology Reports 38, no. 4 (2017): 2480-2488. https://doi.org/10.3892/or.2017.5896
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