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Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells

  • Authors:
    • Jitao Wang
    • Zhi Li
    • Changzeng Zuo
    • Qingfan Xie
    • Hui Li
    • Junhong Jia
    • Zhongguang Zhen
    • Ruizhao Qi
    • Zhiwei Li
    • Dengxiang Liu
    • Baijun Sun
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, The Affiliated Xingtai People's Hospital of Hebei Medical University; Institute of Tumor Disease of Xingtai City, Xingtai, Hebei 054001, P.R. China, Department of Medicine, General Hospital of Jizhong Energy Xingtai Mining Group Limited Liability Company, Xingtai, Hebei 054000, P.R. China, Department of Hepatobiliary Surgery, Beijing 302 Hospital, Beijing 100039, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2166-2172
    |
    Published online on: August 14, 2017
       https://doi.org/10.3892/or.2017.5906
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Abstract

In recent years it was found that the synthesis and biological activity of ribosomes are closely associated with tumor cell growth, tumorigenesis, and malignant transformation. However, the role of regulator of ribosome synthesis 1 (RRS1) in hepatocellular carcinoma (HCC) has not yet been reported. In the present study, we aimed to examine the potential role of RRS1 in tumor cell growth by using a lentivirus-mediated RNA interference (RNAi) system in the HCC cell line SMMC-7721 in vitro. Compared with that of the negative control group (Lv-shCon), the mRNA and protein expression levels of RRS1 in SMMC-7721 cells transfected with Lv-shRRS1 were significantly decreased. Further experiments found that silencing of RRS1 gene expression in SMMC-7721 cells significantly suppressed cell proliferation, inhibited colony formation capacity, increased apoptosis and arrested cells in the G1 phase. These results suggest that the RRS1 gene plays a critical role in cell proliferation, colony formation, cell apoptosis and cell cycle distribution in human HCC cells, and that silencing of RRS1 by RNAi is a promising therapeutic approach for the treatment of HCC, and should be further developed.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Li Z, Zuo C, Xie Q, Li H, Jia J, Zhen Z, Qi R, Li Z, Liu D, Liu D, et al: Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells. Oncol Rep 38: 2166-2172, 2017.
APA
Wang, J., Li, Z., Zuo, C., Xie, Q., Li, H., Jia, J. ... Sun, B. (2017). Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells. Oncology Reports, 38, 2166-2172. https://doi.org/10.3892/or.2017.5906
MLA
Wang, J., Li, Z., Zuo, C., Xie, Q., Li, H., Jia, J., Zhen, Z., Qi, R., Li, Z., Liu, D., Sun, B."Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells". Oncology Reports 38.4 (2017): 2166-2172.
Chicago
Wang, J., Li, Z., Zuo, C., Xie, Q., Li, H., Jia, J., Zhen, Z., Qi, R., Li, Z., Liu, D., Sun, B."Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells". Oncology Reports 38, no. 4 (2017): 2166-2172. https://doi.org/10.3892/or.2017.5906
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Li Z, Zuo C, Xie Q, Li H, Jia J, Zhen Z, Qi R, Li Z, Liu D, Liu D, et al: Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells. Oncol Rep 38: 2166-2172, 2017.
APA
Wang, J., Li, Z., Zuo, C., Xie, Q., Li, H., Jia, J. ... Sun, B. (2017). Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells. Oncology Reports, 38, 2166-2172. https://doi.org/10.3892/or.2017.5906
MLA
Wang, J., Li, Z., Zuo, C., Xie, Q., Li, H., Jia, J., Zhen, Z., Qi, R., Li, Z., Liu, D., Sun, B."Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells". Oncology Reports 38.4 (2017): 2166-2172.
Chicago
Wang, J., Li, Z., Zuo, C., Xie, Q., Li, H., Jia, J., Zhen, Z., Qi, R., Li, Z., Liu, D., Sun, B."Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells". Oncology Reports 38, no. 4 (2017): 2166-2172. https://doi.org/10.3892/or.2017.5906
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