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Killing effect of methionine enkephalin on melanoma in vivo and in vitro

  • Authors:
    • Dong-Mei Wang
    • Xue Jiao
    • Nicolas P. Plotnikoff
    • Noreen Griffin
    • Rui-Qun Qi
    • Xing-Hua Gao
    • Feng-Ping Shan
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, College of Basic Medical Science, China Medical University, Shenyang, Liaoning 110122, P.R. China, Center for Translational Medicine, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China, Immune Therapeutics, Inc., Orlando, FL 32801, USA, Department of Dermatology, No. 1 Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2132-2140
    |
    Published online on: August 24, 2017
       https://doi.org/10.3892/or.2017.5918
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Abstract

Melanoma is a common cutaneous malignancy, that is also found in specific mucosal sites, and is associated with a poor prognosis. The aim of the present study was to investigate the cytotoxicity of methionine enkephalin (MENK) for B16 melanoma cells in vivo and in vitro. The results of the present study allowed our conclusion that MENK regulates the proliferation of B16 cells, causing cell cycle arrest in the G0/G1 phase and a decrease in the percentage of cells in the S and G2/M phases. Reverse transcription-quantitative polymerase chain reaction demonstrated that MENK increased opioid receptor expression in the B16 cells. Furthermore, the tumor volume and weight in the MENK-treated group were lower than those in the control group (NS) and MENK and naltrexone (NTX)-treated groups. MENK exerted both significant antitumor activity on the growth of B16 cells and a longer survival time in mice. The mice treated with MENK exhibited an increased ratio of CD4+ to CD8+ T cells as tested by flow cytometry (FCM), resulting in a ratio of 2.03 in the control group, 3.69 in the MENK-treated group, and 2.65 in the MENK and NTX group. Furthermore, a significant increase in plasma levels of IL-2, IFN-γ and TNF-α was revealed as assessed by ELISA. In conclusion, the results of the present study indicate that MENK has a cytotoxic effect on B16 melanoma cells in vitro and in vivo, and suggest a potential mechanism for these bioactivities. Therefore, we posit that MENK should be investigated, not only as a primary therapy for melanoma, but also as an adjuvant therapy in combination with chemotherapies.
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Copy and paste a formatted citation
Spandidos Publications style
Wang D, Jiao X, Plotnikoff NP, Griffin N, Qi R, Gao X and Shan F: Killing effect of methionine enkephalin on melanoma in vivo and in vitro. Oncol Rep 38: 2132-2140, 2017.
APA
Wang, D., Jiao, X., Plotnikoff, N.P., Griffin, N., Qi, R., Gao, X., & Shan, F. (2017). Killing effect of methionine enkephalin on melanoma in vivo and in vitro. Oncology Reports, 38, 2132-2140. https://doi.org/10.3892/or.2017.5918
MLA
Wang, D., Jiao, X., Plotnikoff, N. P., Griffin, N., Qi, R., Gao, X., Shan, F."Killing effect of methionine enkephalin on melanoma in vivo and in vitro". Oncology Reports 38.4 (2017): 2132-2140.
Chicago
Wang, D., Jiao, X., Plotnikoff, N. P., Griffin, N., Qi, R., Gao, X., Shan, F."Killing effect of methionine enkephalin on melanoma in vivo and in vitro". Oncology Reports 38, no. 4 (2017): 2132-2140. https://doi.org/10.3892/or.2017.5918
Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Jiao X, Plotnikoff NP, Griffin N, Qi R, Gao X and Shan F: Killing effect of methionine enkephalin on melanoma in vivo and in vitro. Oncol Rep 38: 2132-2140, 2017.
APA
Wang, D., Jiao, X., Plotnikoff, N.P., Griffin, N., Qi, R., Gao, X., & Shan, F. (2017). Killing effect of methionine enkephalin on melanoma in vivo and in vitro. Oncology Reports, 38, 2132-2140. https://doi.org/10.3892/or.2017.5918
MLA
Wang, D., Jiao, X., Plotnikoff, N. P., Griffin, N., Qi, R., Gao, X., Shan, F."Killing effect of methionine enkephalin on melanoma in vivo and in vitro". Oncology Reports 38.4 (2017): 2132-2140.
Chicago
Wang, D., Jiao, X., Plotnikoff, N. P., Griffin, N., Qi, R., Gao, X., Shan, F."Killing effect of methionine enkephalin on melanoma in vivo and in vitro". Oncology Reports 38, no. 4 (2017): 2132-2140. https://doi.org/10.3892/or.2017.5918
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