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Article

Chaetocin induces cell cycle arrest and apoptosis by regulating the ROS-mediated ASK-1/JNK signaling pathways

  • Authors:
    • Jingliang He
    • Xiaoxun Chen
    • Bowei Li
    • Wenjie Zhou
    • Jinfeng Xiao
    • Ke He
    • Jinqian Zhang
    • Guoan Xiang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China, Department of Gastrointestinal Surgery, The Guigang City People's Hospital, Guigang, Guangxi 537100, P.R. China, The Third Clinical Medical College of Southern Medical University, Guangzhou, Guangdong 510317, P.R. China, Department of Laboratory Medicine, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China
  • Pages: 2489-2497
    |
    Published online on: August 24, 2017
       https://doi.org/10.3892/or.2017.5921
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Abstract

The present study demonstrated that chaetocin, a natural small-molecule product produced by Chaetomium fungal species and a potential anticancer agent, inhibited the viability and invasive ability of the human intrahepatic cholangio­carcinoma cell line CCLP-1 in vivo and in vitro as revealed by CCK-8 and Transwell invasion assays and mouse xenograft tumor experiments. As determined using flow cytometry and intracellular ROS assays, chaetocin was found to induce cell cycle arrest and oxidative stress, leading to CCLP-1 cell apoptosis. Cell apoptosis can be initiated via different apoptotic signaling pathways under oxidative stress. As determined by western blot analysis, expression levels of the apoptosis signal-regulating kinase 1 (ASK-1) signalosome and its downstream c-Jun N-terminal kinase (JNK) signaling pathway were increased under oxidative stress stimulation. These findings indicate that chaetocin arrests the cell cycle and induces apoptosis by regulating the reactive oxygen species-mediated ASK-1/JNK signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
He J, Chen X, Li B, Zhou W, Xiao J, He K, Zhang J and Xiang G: Chaetocin induces cell cycle arrest and apoptosis by regulating the ROS-mediated ASK-1/JNK signaling pathways. Oncol Rep 38: 2489-2497, 2017.
APA
He, J., Chen, X., Li, B., Zhou, W., Xiao, J., He, K. ... Xiang, G. (2017). Chaetocin induces cell cycle arrest and apoptosis by regulating the ROS-mediated ASK-1/JNK signaling pathways. Oncology Reports, 38, 2489-2497. https://doi.org/10.3892/or.2017.5921
MLA
He, J., Chen, X., Li, B., Zhou, W., Xiao, J., He, K., Zhang, J., Xiang, G."Chaetocin induces cell cycle arrest and apoptosis by regulating the ROS-mediated ASK-1/JNK signaling pathways". Oncology Reports 38.4 (2017): 2489-2497.
Chicago
He, J., Chen, X., Li, B., Zhou, W., Xiao, J., He, K., Zhang, J., Xiang, G."Chaetocin induces cell cycle arrest and apoptosis by regulating the ROS-mediated ASK-1/JNK signaling pathways". Oncology Reports 38, no. 4 (2017): 2489-2497. https://doi.org/10.3892/or.2017.5921
Copy and paste a formatted citation
x
Spandidos Publications style
He J, Chen X, Li B, Zhou W, Xiao J, He K, Zhang J and Xiang G: Chaetocin induces cell cycle arrest and apoptosis by regulating the ROS-mediated ASK-1/JNK signaling pathways. Oncol Rep 38: 2489-2497, 2017.
APA
He, J., Chen, X., Li, B., Zhou, W., Xiao, J., He, K. ... Xiang, G. (2017). Chaetocin induces cell cycle arrest and apoptosis by regulating the ROS-mediated ASK-1/JNK signaling pathways. Oncology Reports, 38, 2489-2497. https://doi.org/10.3892/or.2017.5921
MLA
He, J., Chen, X., Li, B., Zhou, W., Xiao, J., He, K., Zhang, J., Xiang, G."Chaetocin induces cell cycle arrest and apoptosis by regulating the ROS-mediated ASK-1/JNK signaling pathways". Oncology Reports 38.4 (2017): 2489-2497.
Chicago
He, J., Chen, X., Li, B., Zhou, W., Xiao, J., He, K., Zhang, J., Xiang, G."Chaetocin induces cell cycle arrest and apoptosis by regulating the ROS-mediated ASK-1/JNK signaling pathways". Oncology Reports 38, no. 4 (2017): 2489-2497. https://doi.org/10.3892/or.2017.5921
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