Functional expression, characterization, and application of human S100B

Wu,Lei; Zhou,Xuesi; Xiao,Zhigang; Gao,Xiujie; Liu,Ziquan; Zhang,Zhiqing; Wang,Kun; Zhu,Yulin; Ren,Hanlin; Wang,Tianhui

doi:10.3892/or.2017.5922

October-2017 Volume 38 Issue 4

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October-2017 Volume 38 Issue 4

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Article

Functional expression, characterization, and application of human S100B

Authors:
- Lei Wu
- Xuesi Zhou
- Zhigang Xiao
- Xiujie Gao
- Ziquan Liu
- Zhiqing Zhang
- Kun Wang
- Yulin Zhu
- Hanlin Ren
- Tianhui Wang
View Affiliations / Copyright

Affiliations: Performance Medicine Laboratory, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China
Pages: 2309-2316
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Published online on: August 24, 2017

https://doi.org/10.3892/or.2017.5922
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Abstract

The EF-hand calcium-binding protein S100B presents a wide range of biological activities and functions. This binding protein is involved in various human diseases, including cancer, brain trauma and ischemia, neurodegenerative disease (Alzheimer's disease), and psychiatric disorders. In this study, we prepared human S100B protein and its monoclonal antibodies. Human S100B protein was expressed in Escherichia coli, successfully purified by diethylaminoethyl cellulose anion-exchange chromatography, and then identified by western blot analysis. Monoclonal antibodies (mAbs) were produced by the standard hybridoma method and validated by enzyme-linked immunosorbent assay and western blot analysis. The prepared human S100B protein and its mAbs demonstrated potential biological activities. The KD of one mAb is approximately 4.72x10-8 mol/l, and its cross reactivity is low with human S100A4, mouse S100A4, and human S100A1. Recombinant Soluble S100B can promote the migration and invasion of HeLa cells. The expression of S100B protein in tumor tissues can be detected effectively by using the prepared monoclonal antibodies. Increasing concentration of the anti-human S100B mAbs showed a reduced expression of the S100B protein. Subsequently, the expression of p53 increased significantly (P<0.05) in A375 cells. A significant increase in apoptosis in A375 cells was observed with increasing S100B mAb concentration. Results showed that our prepared S100B mAbs were suitable for detecting S100B expression in human tissues, furnishing promising tools for further functional investigation and clinical applications.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Functional expression, characterization, and application of human S100B

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Abstract

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