Inhibition of NOB1 by microRNA-330-5p overexpression represses cell growth of non-small cell lung cancer

  • Authors:
    • Ranran Kong
    • Wei Liu
    • Yurui Guo
    • Jie Feng
    • Chuantao Cheng
    • Xinwu Zhang
    • Yuefeng Ma
    • Shaomin Li
    • Jiantao Jiang
    • Jin Zhang
    • Zhe Qiao
    • Jie Qin
    • Teng Lu
    • Xijing He
  • View Affiliations

  • Published online on: August 28, 2017     https://doi.org/10.3892/or.2017.5927
  • Pages: 2572-2580
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Abstract

MicroRNAs (miRNAs) play critical roles in the development and progression of various cancers, including non-small-cell lung cancer (NSCLC). Studies have suggested that miR-330-5p is involved in the progression of several cancers. However, the role of miR-330-5p in NSCLC remains unclear. We investigated the effect on and mechanism of miR-330-5p in the progression of NSCLC. We found that miR-330-5p was significantly downregulated in NSCLC tissues and cell lines as detected by real-time quantitative polymerase chain reaction (RT-qPCR). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), bromodeoxyuridine (BrdU), colony formation and cell cycle assays showed that overexpression of miR-330-5p markedly inhibited cell growth. Annexin V-FITC/PI and caspase-3 activity assays showed that overexpression of miR-330-5p significantly promoted cell apoptosis of NSCLC cells. Bioinformatics analysis and dual-luciferase reporter assays confirmed NIN/RPN12 binding protein 1 (NOB1) as a target gene of miR-330-5p. RT-qPCR and Western blot analysis showed that overexpression of miR-330-5p inhibited the expression of NOB1 as well as cyclin D1 and cyclin-dependent kinase 4 in NSCLC cells. Moreover, overexpression of NOB1 markedly reversed the miR‑330-5p-mediated inhibitory effect on NSCLC cell growth. Correlation analysis showed that miR‑330-5p expression was inversely correlated with NOB1 mRNA expression in NSCLC tissues. Taken together, our results indicate that miR-330-5p inhibits NSCLC cell growth through downregulation of NOB1 expression. Our study suggests that miR-330-5p may serve as a potential therapeutic target for the treatment of NSCLC.
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October-2017
Volume 38 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kong R, Liu W, Guo Y, Feng J, Cheng C, Zhang X, Ma Y, Li S, Jiang J, Zhang J, Zhang J, et al: Inhibition of NOB1 by microRNA-330-5p overexpression represses cell growth of non-small cell lung cancer. Oncol Rep 38: 2572-2580, 2017
APA
Kong, R., Liu, W., Guo, Y., Feng, J., Cheng, C., Zhang, X. ... He, X. (2017). Inhibition of NOB1 by microRNA-330-5p overexpression represses cell growth of non-small cell lung cancer. Oncology Reports, 38, 2572-2580. https://doi.org/10.3892/or.2017.5927
MLA
Kong, R., Liu, W., Guo, Y., Feng, J., Cheng, C., Zhang, X., Ma, Y., Li, S., Jiang, J., Zhang, J., Qiao, Z., Qin, J., Lu, T., He, X."Inhibition of NOB1 by microRNA-330-5p overexpression represses cell growth of non-small cell lung cancer". Oncology Reports 38.4 (2017): 2572-2580.
Chicago
Kong, R., Liu, W., Guo, Y., Feng, J., Cheng, C., Zhang, X., Ma, Y., Li, S., Jiang, J., Zhang, J., Qiao, Z., Qin, J., Lu, T., He, X."Inhibition of NOB1 by microRNA-330-5p overexpression represses cell growth of non-small cell lung cancer". Oncology Reports 38, no. 4 (2017): 2572-2580. https://doi.org/10.3892/or.2017.5927