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Article

Interaction with neutrophils promotes gastric cancer cell migration and invasion by inducing epithelial-mesenchymal transition

  • Authors:
    • Wen Zhang
    • Jianmei Gu
    • Jingyan Chen
    • Peng Zhang
    • Runbi Ji
    • Hui Qian
    • Wenrong Xu
    • Xu Zhang
  • View Affiliations / Copyright

    Affiliations: Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China, Department of Clinical Laboratory, Nantong Tumor Hospital, Nantong, Jiangsu 226361, P.R. China
  • Pages: 2959-2966
    |
    Published online on: September 6, 2017
       https://doi.org/10.3892/or.2017.5942
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Abstract

Emerging evidence has revealed that neutrophils have phenotypic and functional plasticity. Neutrophils could be polarized towards a pro-tumor phenotype by tumor-derived factors. In the present study, we investigated the role of the interaction with neutrophils on the functions of gastric cancer cells in vitro. Human promyelocytic leukemia HL-60 cells were induced to differentiate into neutrophil-like cells (HL-60N) using dimethyl sulfoxide (DMSO). Human gastric cancer cells were co-cultured with HL-60N cells or treated with the conditioned medium (CM) of cancer-activated HL-60N cells. The migration and invasion of gastric cancer cells were significantly enhanced while their proliferation was minimally altered. The expression of pro-inflammatory factors including IL-6, IL-8, IL-1β, and TNFα was significantly increased in cancer-activated HL-60N cells, which induced the activation of the ERK pathway and epithelial-mesenchymal transition (EMT) in gastric cancer cells. Blocking the ERK pathway activation reversed the promoting effects of cancer-activated HL-60N cells on gastric cancer cell migration and invasion. In addition, mouse gastric cancer cell derived CM could also increase the expression of pro-inflammatory factors in mouse bone marrow neutrophils, which in turn enhanced the migration and invasion of mouse gastric cancer cells. Collectively, our findings revealed that the interaction with neutrophils promoted gastric cancer cell migration and invasion through the activation of the ERK pathway and the induction of EMT, indicating that neutrophils may play an important role in gastric cancer metastasis. Therefore, targeting neutrophil-cancer cell interaction may provide a new strategy for the treatment of gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang W, Gu J, Chen J, Zhang P, Ji R, Qian H, Xu W and Zhang X: Interaction with neutrophils promotes gastric cancer cell migration and invasion by inducing epithelial-mesenchymal transition. Oncol Rep 38: 2959-2966, 2017.
APA
Zhang, W., Gu, J., Chen, J., Zhang, P., Ji, R., Qian, H. ... Zhang, X. (2017). Interaction with neutrophils promotes gastric cancer cell migration and invasion by inducing epithelial-mesenchymal transition. Oncology Reports, 38, 2959-2966. https://doi.org/10.3892/or.2017.5942
MLA
Zhang, W., Gu, J., Chen, J., Zhang, P., Ji, R., Qian, H., Xu, W., Zhang, X."Interaction with neutrophils promotes gastric cancer cell migration and invasion by inducing epithelial-mesenchymal transition". Oncology Reports 38.5 (2017): 2959-2966.
Chicago
Zhang, W., Gu, J., Chen, J., Zhang, P., Ji, R., Qian, H., Xu, W., Zhang, X."Interaction with neutrophils promotes gastric cancer cell migration and invasion by inducing epithelial-mesenchymal transition". Oncology Reports 38, no. 5 (2017): 2959-2966. https://doi.org/10.3892/or.2017.5942
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang W, Gu J, Chen J, Zhang P, Ji R, Qian H, Xu W and Zhang X: Interaction with neutrophils promotes gastric cancer cell migration and invasion by inducing epithelial-mesenchymal transition. Oncol Rep 38: 2959-2966, 2017.
APA
Zhang, W., Gu, J., Chen, J., Zhang, P., Ji, R., Qian, H. ... Zhang, X. (2017). Interaction with neutrophils promotes gastric cancer cell migration and invasion by inducing epithelial-mesenchymal transition. Oncology Reports, 38, 2959-2966. https://doi.org/10.3892/or.2017.5942
MLA
Zhang, W., Gu, J., Chen, J., Zhang, P., Ji, R., Qian, H., Xu, W., Zhang, X."Interaction with neutrophils promotes gastric cancer cell migration and invasion by inducing epithelial-mesenchymal transition". Oncology Reports 38.5 (2017): 2959-2966.
Chicago
Zhang, W., Gu, J., Chen, J., Zhang, P., Ji, R., Qian, H., Xu, W., Zhang, X."Interaction with neutrophils promotes gastric cancer cell migration and invasion by inducing epithelial-mesenchymal transition". Oncology Reports 38, no. 5 (2017): 2959-2966. https://doi.org/10.3892/or.2017.5942
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