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Article

Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway

  • Authors:
    • Jun Gao
    • Hong Zhu
    • Hong Wan
    • Xia Zou
    • Xiaoxin Ma
    • Guolan Gao
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R. China, Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, P.R. China, Department of Obstetrics and Gynecology, Aviation General Hospital of China Medical University, Beijing, P.R. China
  • Pages: 2927-2934
    |
    Published online on: September 13, 2017
       https://doi.org/10.3892/or.2017.5952
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Abstract

Ovarian cancer is the most lethal gynaecological cancer and the sixth most common cause of cancer related death among Western women. Recent studies show that harmine, a small-molecular β-carboline alkaloid present in medicinal plants, displayed obvious anticancer effects in several cancer cells. However, the effect of harmine on ovarian cancer is not well understood. In the present study, the effect of harmine on the cell proliferation and migration of ovarian cancer SKOV-3 cells and the underlying mechanism were investigated. Our results indicated that harmine significantly suppressed the proliferation of SKOV-3 cells in a dose-dependent manner. Interestingly, it also inhibited the epidermal growth factor (EGF)-induced proliferation of SKOV-3 cells. Moreover, the migration of SKOV-3 cells was markedly inhibited by harmine treatment. Further study showed that harmine inhibited not only the basal phosphorylation level of extra­cellular signal-regulated kinase 1/2 (ERK1/2) and cyclic adenosine monophosphate response element-binding protein (CREB) but also EGF-induced ERK1/2 and CREB phosphorylation. Finally, harmine significantly suppressed the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP) family MMP-2, and MMP-9. In conclusion, our data revealed that harmine inhibited the proliferation and migration of SKOV-3 cells, which might be mediated by ERK/CREB pathway. These findings elucidate that harmine may act as a potential therapeutic drug for ovarian cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Gao J, Zhu H, Wan H, Zou X, Ma X and Gao G: Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway. Oncol Rep 38: 2927-2934, 2017.
APA
Gao, J., Zhu, H., Wan, H., Zou, X., Ma, X., & Gao, G. (2017). Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway. Oncology Reports, 38, 2927-2934. https://doi.org/10.3892/or.2017.5952
MLA
Gao, J., Zhu, H., Wan, H., Zou, X., Ma, X., Gao, G."Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway". Oncology Reports 38.5 (2017): 2927-2934.
Chicago
Gao, J., Zhu, H., Wan, H., Zou, X., Ma, X., Gao, G."Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway". Oncology Reports 38, no. 5 (2017): 2927-2934. https://doi.org/10.3892/or.2017.5952
Copy and paste a formatted citation
x
Spandidos Publications style
Gao J, Zhu H, Wan H, Zou X, Ma X and Gao G: Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway. Oncol Rep 38: 2927-2934, 2017.
APA
Gao, J., Zhu, H., Wan, H., Zou, X., Ma, X., & Gao, G. (2017). Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway. Oncology Reports, 38, 2927-2934. https://doi.org/10.3892/or.2017.5952
MLA
Gao, J., Zhu, H., Wan, H., Zou, X., Ma, X., Gao, G."Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway". Oncology Reports 38.5 (2017): 2927-2934.
Chicago
Gao, J., Zhu, H., Wan, H., Zou, X., Ma, X., Gao, G."Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway". Oncology Reports 38, no. 5 (2017): 2927-2934. https://doi.org/10.3892/or.2017.5952
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