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Article

1H-NMR-based metabolic profiling of a colorectal cancer CT-26 lung metastasis model in mice

  • Authors:
    • Yan Li
    • Chunting Wang
    • Dandan Li
    • Pengchi Deng
    • Xiaoni Shao
    • Jing Hu
    • Chunqi Liu
    • Hui Jie
    • Yiyun Lin
    • Zhuoling Li
    • Xinying Qian
    • Huaqin Zhang
    • Yinglan Zhao
  • View Affiliations / Copyright

    Affiliations: Pharmacodynamics Pharmacokinetics Early Safety Evaluation Model Animals, Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, Sichuan 610041, P.R. China, Analytical and Testing Center, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 3044-3054
    |
    Published online on: September 13, 2017
       https://doi.org/10.3892/or.2017.5954
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Abstract

Lung metastasis is an important cause for the low 5-year survival rate of colorectal cancer patients. Understanding the metabolic profile of lung metastasis of colorectal cancer is important for developing molecular diagnostic and therapeutic approaches. We carried out the metabonomic profiling of lung tissue samples on a mouse lung metastasis model of colorectal cancer using 1H-nuclear magnetic resonance (1H-NMR). The lung tissues of mice were collected at different intervals after marine colon cancer cell line CT-26 was intravenously injected into BALB/c mice. The distinguishing metabolites of lung tissue were investigated using 1H-NMR-based metabonomic assay, which is a highly sensitive and non-destructive method for biomarker identification. Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were applied to analyze 1H-NMR profiling data to seek potential biomarkers. All of the 3 analyses achieved excellent separations between the normal and metastasis groups. A total of 42 metabolites were identified, ~12 of which were closely correlated with the process of metastasis from colon to lung. These altered metabolites indicated the disturbance of metabolism in metastatic tumors including glycolysis, TCA cycle, glutaminolysis, choline metabolism and serine biosynthesis. Our findings firstly identified the distinguishing metabolites in mouse colorectal cancer lung metastasis models, and indicated that the metabolite disturbance may be associated with the progression of lung metastasis from colon cancer. The altered metabolites may be potential biomarkers that provide a promising molecular approach for clinical diagnosis and mechanistic study of colorectal cancer with lung metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Wang C, Li D, Deng P, Shao X, Hu J, Liu C, Jie H, Lin Y, Li Z, Li Z, et al: 1H-NMR-based metabolic profiling of a colorectal cancer CT-26 lung metastasis model in mice. Oncol Rep 38: 3044-3054, 2017.
APA
Li, Y., Wang, C., Li, D., Deng, P., Shao, X., Hu, J. ... Zhao, Y. (2017). 1H-NMR-based metabolic profiling of a colorectal cancer CT-26 lung metastasis model in mice. Oncology Reports, 38, 3044-3054. https://doi.org/10.3892/or.2017.5954
MLA
Li, Y., Wang, C., Li, D., Deng, P., Shao, X., Hu, J., Liu, C., Jie, H., Lin, Y., Li, Z., Qian, X., Zhang, H., Zhao, Y."1H-NMR-based metabolic profiling of a colorectal cancer CT-26 lung metastasis model in mice". Oncology Reports 38.5 (2017): 3044-3054.
Chicago
Li, Y., Wang, C., Li, D., Deng, P., Shao, X., Hu, J., Liu, C., Jie, H., Lin, Y., Li, Z., Qian, X., Zhang, H., Zhao, Y."1H-NMR-based metabolic profiling of a colorectal cancer CT-26 lung metastasis model in mice". Oncology Reports 38, no. 5 (2017): 3044-3054. https://doi.org/10.3892/or.2017.5954
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Wang C, Li D, Deng P, Shao X, Hu J, Liu C, Jie H, Lin Y, Li Z, Li Z, et al: 1H-NMR-based metabolic profiling of a colorectal cancer CT-26 lung metastasis model in mice. Oncol Rep 38: 3044-3054, 2017.
APA
Li, Y., Wang, C., Li, D., Deng, P., Shao, X., Hu, J. ... Zhao, Y. (2017). 1H-NMR-based metabolic profiling of a colorectal cancer CT-26 lung metastasis model in mice. Oncology Reports, 38, 3044-3054. https://doi.org/10.3892/or.2017.5954
MLA
Li, Y., Wang, C., Li, D., Deng, P., Shao, X., Hu, J., Liu, C., Jie, H., Lin, Y., Li, Z., Qian, X., Zhang, H., Zhao, Y."1H-NMR-based metabolic profiling of a colorectal cancer CT-26 lung metastasis model in mice". Oncology Reports 38.5 (2017): 3044-3054.
Chicago
Li, Y., Wang, C., Li, D., Deng, P., Shao, X., Hu, J., Liu, C., Jie, H., Lin, Y., Li, Z., Qian, X., Zhang, H., Zhao, Y."1H-NMR-based metabolic profiling of a colorectal cancer CT-26 lung metastasis model in mice". Oncology Reports 38, no. 5 (2017): 3044-3054. https://doi.org/10.3892/or.2017.5954
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