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November-2017 Volume 38 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Overexpression of RAB34 correlates with poor prognosis and tumor progression in hepatocellular carcinoma

  • Authors:
    • Jianwu Wu
    • Yijie Lu
    • Ancheng Qin
    • Zhiming Qiao
    • Xinwei Jiang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Nanjing Medical University, Affiliated Suzhou Hospital, Gusu, Suzhou, Jiangsu 215000, P.R. China
  • Pages: 2967-2974
    |
    Published online on: September 18, 2017
       https://doi.org/10.3892/or.2017.5957
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Abstract

RAB34, a protein belonging to the RAB family, is involved in protein transport, repositioning of lysosomes and activation of micropinocytosis. However, few studies have reported its function in human epithelial cancers. Immunohistochemistry (IHC) and western blotting were used to detect expression of RAB34 at the tissue and cell levels. Cell Counting Kit-8 (CCK-8), EDU assay and flow cytometry were used for analyzing cell proliferation. Transwell and scratch wound healing assays were used for assessing cell migration ability. Western blotting was used for detecting expression of E-cadherin and N-cadherin. In the present study, we found that both DNA copy and protein level of RAB34 were upregulating in human hepatocellular carcinoma (HCC) tissues when compared with that in adjacent tissues. Analysis of the correlation between RAB34 expression and clinicopathological features showed that patients with overexpression of RAB34 consistently had large tumor size, vessel invasion and poor tumor grade. Furthermore, overall survival analysis showed that patients with upregulated expression of RAB34 were associated with poor prognosis. Moreover, cell function experiments showed that suppression of RAB34 led to a lower proliferation rate and migration ability. In addition, this phenomenon may be attributed to cell cycle phase G1 arrest and mesenchymal-epithelial transition under condition of RAB34 suppression. The present study demonstrated that RAB34 plays an important role in the initiation and progression of HCC. Our results suggest a new therapeutic target for the clinical treatment of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Wu J, Lu Y, Qin A, Qiao Z and Jiang X: Overexpression of RAB34 correlates with poor prognosis and tumor progression in hepatocellular carcinoma. Oncol Rep 38: 2967-2974, 2017.
APA
Wu, J., Lu, Y., Qin, A., Qiao, Z., & Jiang, X. (2017). Overexpression of RAB34 correlates with poor prognosis and tumor progression in hepatocellular carcinoma. Oncology Reports, 38, 2967-2974. https://doi.org/10.3892/or.2017.5957
MLA
Wu, J., Lu, Y., Qin, A., Qiao, Z., Jiang, X."Overexpression of RAB34 correlates with poor prognosis and tumor progression in hepatocellular carcinoma". Oncology Reports 38.5 (2017): 2967-2974.
Chicago
Wu, J., Lu, Y., Qin, A., Qiao, Z., Jiang, X."Overexpression of RAB34 correlates with poor prognosis and tumor progression in hepatocellular carcinoma". Oncology Reports 38, no. 5 (2017): 2967-2974. https://doi.org/10.3892/or.2017.5957
Copy and paste a formatted citation
x
Spandidos Publications style
Wu J, Lu Y, Qin A, Qiao Z and Jiang X: Overexpression of RAB34 correlates with poor prognosis and tumor progression in hepatocellular carcinoma. Oncol Rep 38: 2967-2974, 2017.
APA
Wu, J., Lu, Y., Qin, A., Qiao, Z., & Jiang, X. (2017). Overexpression of RAB34 correlates with poor prognosis and tumor progression in hepatocellular carcinoma. Oncology Reports, 38, 2967-2974. https://doi.org/10.3892/or.2017.5957
MLA
Wu, J., Lu, Y., Qin, A., Qiao, Z., Jiang, X."Overexpression of RAB34 correlates with poor prognosis and tumor progression in hepatocellular carcinoma". Oncology Reports 38.5 (2017): 2967-2974.
Chicago
Wu, J., Lu, Y., Qin, A., Qiao, Z., Jiang, X."Overexpression of RAB34 correlates with poor prognosis and tumor progression in hepatocellular carcinoma". Oncology Reports 38, no. 5 (2017): 2967-2974. https://doi.org/10.3892/or.2017.5957
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