miR-504 promotes tumour growth and metastasis in human osteosarcoma by targeting TP53INP1

  • Authors:
    • Qingchun Cai
    • Sixiang Zeng
    • Xing Dai
    • Junlong Wu
    • Wei Ma
  • View Affiliations

  • Published online on: September 21, 2017     https://doi.org/10.3892/or.2017.5983
  • Pages: 2993-3000
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Abstract

An increasing number of studies have demonstrated that microRNAs participate in the development of osteosarcoma by acting as tumour suppressor or tumour-promoting genes. We investigated the role of miR-504 in the growth and metastasis of osteosarcoma. The expression of miR-504 in clinical osteosarcoma samples was higher than that in the adjacent normal tissue and correlated with tumour size and clinical stage. Tumour protein p53-inducible nuclear protein 1 (TP53INP1) was downregulated in the clinical osteosarcoma samples compared with the adjacent normal tissues and was consistently correlated with the clinical stage. The results of dual-luciferase reporter assay and western blot analysis demonstrated that the TP53INP1 gene is a direct target of miR-504. Altogether, the Cell Counting Kit-8 (CCK-8), the colony formation, the flow cytometry and the Transwell assay results demonstrated that miR-504 promoted osteosarcoma cell growth and metastasis in vitro. P73, P21, Bax, cleaved-caspase-3 and secreted protein acidic and rich in cysteine (SPARC) were associated with the suppressive role of miR-504/TP53INP1. The overexpression of miR-504 in osteosarcoma xenografts enhanced the tumour growth and increased the metastatic burden. Collectively, these results revealed that TP53INP1 is a target gene of miR-504 and that miR-504 enhances osteosarcoma growth and promotes distant metastases by targeting TP53INP1. Thus, miR-504/TP53INP1 may be associated with osteosarcoma size and clinical stage.
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November-2017
Volume 38 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cai Q, Zeng S, Dai X, Wu J and Ma W: miR-504 promotes tumour growth and metastasis in human osteosarcoma by targeting TP53INP1. Oncol Rep 38: 2993-3000, 2017
APA
Cai, Q., Zeng, S., Dai, X., Wu, J., & Ma, W. (2017). miR-504 promotes tumour growth and metastasis in human osteosarcoma by targeting TP53INP1. Oncology Reports, 38, 2993-3000. https://doi.org/10.3892/or.2017.5983
MLA
Cai, Q., Zeng, S., Dai, X., Wu, J., Ma, W."miR-504 promotes tumour growth and metastasis in human osteosarcoma by targeting TP53INP1". Oncology Reports 38.5 (2017): 2993-3000.
Chicago
Cai, Q., Zeng, S., Dai, X., Wu, J., Ma, W."miR-504 promotes tumour growth and metastasis in human osteosarcoma by targeting TP53INP1". Oncology Reports 38, no. 5 (2017): 2993-3000. https://doi.org/10.3892/or.2017.5983