Myeloid zinc finger 1 protein is a key transcription stimulating factor of PYROXD2 promoter

Liu,Huilin; Jiang,Xingyan; Wang,Tao; Yu,Fang; Wang,Xingzhi; Chen,Jiaojiao; Xie,Xiaoyuan; Fan,Handong

doi:10.3892/or.2017.5990

November-2017 Volume 38 Issue 5

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November-2017 Volume 38 Issue 5

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Article

Myeloid zinc finger 1 protein is a key transcription stimulating factor of PYROXD2 promoter

Authors:
- Huilin Liu
- Xingyan Jiang
- Tao Wang
- Fang Yu
- Xingzhi Wang
- Jiaojiao Chen
- Xiaoyuan Xie
- Handong Fan
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Affiliations: Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang 310036, P.R. China, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, P.R. China
Pages: 3245-3253
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Published online on: September 22, 2017

https://doi.org/10.3892/or.2017.5990
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Abstract

Previous studies revealed that PYROXD2 was more highly expressed in normal liver tissue and liver cell lines than in cancer tissue and cancer cell lines, which indicated that decreased PYROXD2 expression may be involved in hepatocarcinogenesis. To identify the mechanisms which regulate PYROXD2 gene transcription, we constructed a series of luciferase reporter plasmids and used them to perform luciferase‑based reporter assays with HepG2, Sk-hep1, L02 and 293T cells with the purpose of characterizing the PYROXD2 reporter region. Our results revealed that the transcription factor myeloid zinc finger 1 (MZF1) is necessary for PYROXD2 gene transcription and that it functions as a trans-activator. DNA binding assays revealed that the MZF1 protein binds to the cis-element TGGGGA located in the -320/-312 region of the PYROXD2 promoter. This promoter had a significantly enhanced activity when the MZF1 protein was overexpressed and a significantly decreased activity when the MZF1 protein expression was silenced. However, MZF1 gene expression was not significantly correlated with PYROXD2 protein expression in the samples of resected tumor tissues, which revealed that the PYROXD2 promoter transcription activity was determined by the aggregated effect of numerous transcription factors. This finding may be helpful in understanding the underlying mechanism which regulates the PYROXD2 expression.

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1	Zhang JL, Zhao WG, Wu KL, Wang K, Zhang X, Gu CF, Li Y, Zhu Y and Wu JG: Human hepatitis B virus X protein promotes cell proliferation and inhibits cell apoptosis through interacting with a serine protease Hepsin. Arch Virol. 150:721–741. 2005. View Article : Google Scholar : PubMed/NCBI
2	Huang J, Wu K, Zhang J, Si W, Zhu Y and Wu J: Putative tumor suppressor YueF affects the functions of hepatitis B virus X protein in hepatoma cell apoptosis and p53 expression. Biotechnol Lett. 30:235–242. 2008. View Article : Google Scholar : PubMed/NCBI
3	Huang HW, Peng JP and Zhang J: YueF overexpression inhibits cell proliferation partly through p21 upregulation in renal cell carcinoma. Int J Mol Sci. 12:2477–2487. 2011. View Article : Google Scholar : PubMed/NCBI
4	Deng Y, Wang J, Wang G, Jin Y, Luo X, Xia X, Gong J and Hu J: p55PIK transcriptionally activated by MZF1 promotes colorectal cancer cell proliferation. Biomed Res Int. 2013:8681312013. View Article : Google Scholar : PubMed/NCBI
5	Eguchi T, Prince T, Wegiel B and Calderwood SK: Role and regulation of myeloid zinc finger protein 1 in cancer. J Cell Biochem. 116:2146–2154. 2015. View Article : Google Scholar : PubMed/NCBI
6	Hsieh YH, Wu TT, Huang CY, Hsieh YS and Liu JY: Suppression of tumorigenicity of human hepatocellular carcinoma cells by antisense oligonucleotide MZF-1. Chin J Physiol. 50:9–15. 2007.PubMed/NCBI
7	Asiedu MK, Beauchamp-Perez FD, Ingle JN, Behrens MD, Radisky DC and Knutson KL: AXL induces epithelial-to-mesenchymal transition and regulates the function of breast cancer stem cells. Oncogene. 33:1316–1324. 2014. View Article : Google Scholar : PubMed/NCBI
8	Mudduluru G, Vajkoczy P and Allgayer H: Myeloid zinc finger 1 induces migration, invasion, and in vivo metastasis through Axl gene expression in solid cancer. Mol Cancer Res. 8:159–169. 2010. View Article : Google Scholar : PubMed/NCBI
9	Jensen K, Shiels C and Freemont PS: PML protein isoforms and the RBCC/TRIM motif. Oncogene. 20:7223–7233. 2001. View Article : Google Scholar : PubMed/NCBI
10	Dellaire G and Bazett-Jones DP: PML nuclear bodies: Dynamic sensors of DNA damage and cellular stress. BioEssays. 26:963–977. 2004. View Article : Google Scholar : PubMed/NCBI
11	Van Damme E, Laukens K, Dang TH and Van Ostade X: A manually curated network of the PML nuclear body interactome reveals an important role for PML-NBs in SUMOylation dynamics. Int J Biol Sci. 6:51–67. 2010. View Article : Google Scholar : PubMed/NCBI
12	Edelstein LC and Collins T: The SCAN domain family of zinc finger transcription factors. Gene. 359:1–17. 2005. View Article : Google Scholar : PubMed/NCBI
13	Driver J, Weber CE, Callaci JJ, Kothari AN, Zapf MA, Roper PM, Borys D, Franzen CA, Gupta GN, Wai PY, et al: Alcohol inhibits osteopontin-dependent transforming growth factor-β1 expression in human mesenchymal stem cells. J Biol Chem. 290:9959–9973. 2015. View Article : Google Scholar : PubMed/NCBI
14	Massagué J: A very private TGF-beta receptor embrace. Mol Cell. 29:149–150. 2008. View Article : Google Scholar : PubMed/NCBI
15	Stielow B, Krüger I, Diezko R, Finkernagel F, Gillemans N, Kong-a-San J, Philipsen S and Suske G: Epigenetic silencing of spermatocyte-specific and neuronal genes by SUMO modification of the transcription factor Sp3. PLoS Genet. 6:e10012032010. View Article : Google Scholar : PubMed/NCBI
16	Plotz G, Raedle J, Brieger A, Trojan J and Zeuzem S: hMutSalpha forms an ATP-dependent complex with hMutLalpha and hMutLbeta on DNA. Nucleic Acids Res. 30:711–718. 2002. View Article : Google Scholar : PubMed/NCBI
17	Sahoo A and Im SH: Molecular mechanisms governing IL-24 gene expression. Immune Netw. 12:1–7. 2012. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Myeloid zinc finger 1 protein is a key transcription stimulating factor of PYROXD2 promoter

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