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Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells

  • Authors:
    • Mingju Xu
    • Xioawei Tang
    • Jinjin Guo
    • Wangbang Sun
    • Faqing Tang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory of Zhuhai Hospital, Jinan University and Zhuhai People's Hospital, Zhuhai, Guangdong 519000, P.R. China, Metallurgical Science and Engineering, Central South University, Changsha, Hunan 410083, P.R. China, Zhuhai Campus, Zunyi Medical College, Zhuhai, Guangdong 519041, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2843-2851
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    Published online on: September 26, 2017
       https://doi.org/10.3892/or.2017.6002
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Abstract

Interleukin-24 (IL-24) is a tumor-suppressor gene that has been documented in human melanoma cells. IL-24 has marked antitumor activities on various types of human cancer, but its underlying mechanism remains unclear. In the present, we investigated the effects of human IL-24 (hIL-24) on the chemotherapy resistance of lung cancer cells. The cisplatin (DDP)-resistant lung carcinoma cell line A549/DDP was subjected to adenovirus-mediated transfection with the human IL-24 gene (Ad-hIL-24). The growth-inhibitory and apoptotic effects of Ad-hIL-24 on A549/DDP cells were observed, and the expression levels of AKT, phosphorylated-AKT (p-AKT) and P-glycoprotein (P-gp) were detected. Ad-hIL-24 significantly decreased the levels of p-AKT and P-gp, and effectively inhibited A549/DDP cell growth. Furthermore, A549/DDP cells exhibited a significantly increased rate of apoptosis, as well as G2/M-phase arrest, following transfection with Ad-hIL-24, and these effects were increased in cells treated with Ad-IL-24 combined with DDP when compared with those treated with Ad-hIL-24 or DDP alone. These results suggest that hIL-24 can reverse the DDP resistance of lung cancer cells, and that the associated mechanism involves the induction of apoptosis and G2/M-phase arrest through the phosphoinositide3-kinase (PI3K)/AKT signaling pathway, as well as a decrease in drug resistance through P-gp expression.
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Copy and paste a formatted citation
Spandidos Publications style
Xu M, Tang X, Guo J, Sun W and Tang F: Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells. Oncol Rep 38: 2843-2851, 2017.
APA
Xu, M., Tang, X., Guo, J., Sun, W., & Tang, F. (2017). Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells. Oncology Reports, 38, 2843-2851. https://doi.org/10.3892/or.2017.6002
MLA
Xu, M., Tang, X., Guo, J., Sun, W., Tang, F."Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells". Oncology Reports 38.5 (2017): 2843-2851.
Chicago
Xu, M., Tang, X., Guo, J., Sun, W., Tang, F."Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells". Oncology Reports 38, no. 5 (2017): 2843-2851. https://doi.org/10.3892/or.2017.6002
Copy and paste a formatted citation
x
Spandidos Publications style
Xu M, Tang X, Guo J, Sun W and Tang F: Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells. Oncol Rep 38: 2843-2851, 2017.
APA
Xu, M., Tang, X., Guo, J., Sun, W., & Tang, F. (2017). Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells. Oncology Reports, 38, 2843-2851. https://doi.org/10.3892/or.2017.6002
MLA
Xu, M., Tang, X., Guo, J., Sun, W., Tang, F."Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells". Oncology Reports 38.5 (2017): 2843-2851.
Chicago
Xu, M., Tang, X., Guo, J., Sun, W., Tang, F."Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells". Oncology Reports 38, no. 5 (2017): 2843-2851. https://doi.org/10.3892/or.2017.6002
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