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Article

Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo

  • Authors:
    • Xinwen Wang
    • Weifeng Xu
    • Shenglin Wang
    • Feqiang Yu
    • Jinyi Feng
    • Xinwu Wang
    • Lurong Zhang
    • Jianhua Lin
  • View Affiliations / Copyright

    Affiliations: The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, Department of Orthopaedics, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China, Department of Orthopaedics, The First Hospital of Putian City, Putian, Fujian 351100, P.R. China
  • Pages: 3153-3159
    |
    Published online on: September 26, 2017
       https://doi.org/10.3892/or.2017.6005
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Abstract

The transdifferentiation of cancer cells into other types of cells in several types of tissues or organs has been studied. However, whether human osteosarcoma MNNG/HOS cells can transdifferentiate into other types of cells has seldom been reported. Meanwhile, the mechanism of tumor angiogenesis is still disputed, and whether MNNG/HOS cells participate in angiogenesis in osteosarcoma remains unknown. In the present study, the investigation was divided into two parts: in vitro and in vivo. In vitro, we cultivated MNNG/HOS cells under hypoxic conditions for 4 days and found that they typically showed a characteristic ‘flagstone’ appearance as cultured vascular endothelial cells (VECs). MNNG/HOS cells that were cultivated on Matrigel under hypoxic conditions gradually formed tubular-like structures. Furthermore, when cultured under hypoxic conditions for 4 days, MNNG/HOS cells also transcribed and expressed several molecular markers of VECs (CD31, CD34 and vWF). In vivo, MNNG/HOS cells (1x106 cells) were cultivated under hypoxic conditions and subcutaneously injected into nude mice; the mice were sacrificed 49 days after inoculation. Immunohistochemical staining with anti-human CD31 antibody showed evidence of tumor angiogenesis in human osteosarcoma MNNG/HOS cells. The results demonstrated that MNNG/HOS cells can transdifferentiate into vascular endothelial cell-like cells in vitro and in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Xu W, Wang S, Yu F, Feng J, Wang X, Zhang L and Lin J: Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo. Oncol Rep 38: 3153-3159, 2017.
APA
Wang, X., Xu, W., Wang, S., Yu, F., Feng, J., Wang, X. ... Lin, J. (2017). Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo. Oncology Reports, 38, 3153-3159. https://doi.org/10.3892/or.2017.6005
MLA
Wang, X., Xu, W., Wang, S., Yu, F., Feng, J., Wang, X., Zhang, L., Lin, J."Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo". Oncology Reports 38.5 (2017): 3153-3159.
Chicago
Wang, X., Xu, W., Wang, S., Yu, F., Feng, J., Wang, X., Zhang, L., Lin, J."Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo". Oncology Reports 38, no. 5 (2017): 3153-3159. https://doi.org/10.3892/or.2017.6005
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Xu W, Wang S, Yu F, Feng J, Wang X, Zhang L and Lin J: Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo. Oncol Rep 38: 3153-3159, 2017.
APA
Wang, X., Xu, W., Wang, S., Yu, F., Feng, J., Wang, X. ... Lin, J. (2017). Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo. Oncology Reports, 38, 3153-3159. https://doi.org/10.3892/or.2017.6005
MLA
Wang, X., Xu, W., Wang, S., Yu, F., Feng, J., Wang, X., Zhang, L., Lin, J."Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo". Oncology Reports 38.5 (2017): 3153-3159.
Chicago
Wang, X., Xu, W., Wang, S., Yu, F., Feng, J., Wang, X., Zhang, L., Lin, J."Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo". Oncology Reports 38, no. 5 (2017): 3153-3159. https://doi.org/10.3892/or.2017.6005
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