Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo

  • Authors:
    • Xinwen Wang
    • Weifeng Xu
    • Shenglin Wang
    • Feqiang Yu
    • Jinyi Feng
    • Xinwu Wang
    • Lurong Zhang
    • Jianhua Lin
  • View Affiliations

  • Published online on: September 26, 2017     https://doi.org/10.3892/or.2017.6005
  • Pages: 3153-3159
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Abstract

The transdifferentiation of cancer cells into other types of cells in several types of tissues or organs has been studied. However, whether human osteosarcoma MNNG/HOS cells can transdifferentiate into other types of cells has seldom been reported. Meanwhile, the mechanism of tumor angiogenesis is still disputed, and whether MNNG/HOS cells participate in angiogenesis in osteosarcoma remains unknown. In the present study, the investigation was divided into two parts: in vitro and in vivo. In vitro, we cultivated MNNG/HOS cells under hypoxic conditions for 4 days and found that they typically showed a characteristic ‘flagstone’ appearance as cultured vascular endothelial cells (VECs). MNNG/HOS cells that were cultivated on Matrigel under hypoxic conditions gradually formed tubular-like structures. Furthermore, when cultured under hypoxic conditions for 4 days, MNNG/HOS cells also transcribed and expressed several molecular markers of VECs (CD31, CD34 and vWF). In vivo, MNNG/HOS cells (1x106 cells) were cultivated under hypoxic conditions and subcutaneously injected into nude mice; the mice were sacrificed 49 days after inoculation. Immunohistochemical staining with anti-human CD31 antibody showed evidence of tumor angiogenesis in human osteosarcoma MNNG/HOS cells. The results demonstrated that MNNG/HOS cells can transdifferentiate into vascular endothelial cell-like cells in vitro and in vivo.
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November-2017
Volume 38 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang X, Xu W, Wang S, Yu F, Feng J, Wang X, Zhang L and Lin J: Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo. Oncol Rep 38: 3153-3159, 2017
APA
Wang, X., Xu, W., Wang, S., Yu, F., Feng, J., Wang, X. ... Lin, J. (2017). Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo. Oncology Reports, 38, 3153-3159. https://doi.org/10.3892/or.2017.6005
MLA
Wang, X., Xu, W., Wang, S., Yu, F., Feng, J., Wang, X., Zhang, L., Lin, J."Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo". Oncology Reports 38.5 (2017): 3153-3159.
Chicago
Wang, X., Xu, W., Wang, S., Yu, F., Feng, J., Wang, X., Zhang, L., Lin, J."Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo". Oncology Reports 38, no. 5 (2017): 3153-3159. https://doi.org/10.3892/or.2017.6005