Baicalein inhibits tumor progression by inhibiting tumor cell growth and tumor angiogenesis

Park,Yang-Gyu; Choi,Jawun; Jung,Hye-Kang; Kim,Bumseok; Kim,Chan; Park,Sang-Youel; Seol,Jae-Won

doi:10.3892/or.2017.6007

Baicalein inhibits tumor progression by inhibiting tumor cell growth and tumor angiogenesis

Authors:
- Yang-Gyu Park
- Jawun Choi
- Hye-Kang Jung
- Bumseok Kim
- Chan Kim
- Sang-Youel Park
- Jae-Won Seol
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Affiliations: Biosafety Research Institute, College of Veterinary Medicine, Chonbuk National University, Iksana, Republic of Korea, CHA Bundang Medical Center, CHA University, Seongnam, Republic of Korea
Published online on: September 26, 2017 https://doi.org/10.3892/or.2017.6007
Pages: 3011-3018

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Abstract

Baicalein, a herbal medicine, is a natural flavonoid isolated from the roots of Scutellaria baicalensis Georgi. It is known for its anticancer, anti-inflammatory and neuroprotective properties. Despite these well-known properties, it is not yet clear what effect baicalein has on tumor progression. Therefore, in the present study, we used B16F10 cells, Lewis lung carcinoma (LLC) cells, and human umbilical vein endothelial cells (HUVECs) to investigate the effect of baicalein on cell proliferation and viability, migration and tube formation in vitro. In addition, an experimental animal model was used to observe the growth rate and metastasis of tumors and tumor vessel formation in vivo. Our results showed that baicalein decreased the proliferation and migration and induced tumor cell death via caspase-3 activation in the B16F10 and LLC cells, and strongly inhibited tube formation and cell migration in HUVECs. Furthermore, mouse models showed that baicalein reduced the tumor volume and greatly reduced the tumor growth rate in the early stages of tumor progression, and the baicalein-treated groups had significantly reduced expression of CD31 (endothelial cell marker) and α-SMA (mural cell marker) in the tumors, indicating that baicalein inhibits tumor angiogenesis by disrupting tumor vasculature development. Comparison of the lymph node and lung samples collected from the baicalein-treated group, and the untreated group showed that baicalein reduced metastasis of the tumor to these tissues. In summary, baicalein reduced tumor progression and metastasis, directly induced tumor cell death, and inhibited tumor angiogenesis. Our results strongly demonstrate that baicalein is a potential chemotherapeutic agent.

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