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Article

Leptin induces ROS via NOX5 in healthy and neoplastic mammary epithelial cells

  • Authors:
    • Sinda Mahbouli
    • Audrey Der Vartanian
    • Sophie Ortega
    • Stéphanie Rougé
    • Marie-Paule Vasson
    • Adrien Rossary
  • View Affiliations / Copyright

    Affiliations: University of Clermont Auvergne, INRA, UNH, Human Nutrition Unit, CRNH Auvergne, F-63000 Clermont-Ferrand, France
  • Pages: 3254-3264
    |
    Published online on: September 27, 2017
       https://doi.org/10.3892/or.2017.6009
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Abstract

NADPH oxidase (NOX) complexes (a family of seven isoforms) drive cellular ROS production in patho­logical processes such as cancer. NOX-driven ROS production is involved in cell mechanisms from signalling to oxidative stress. Leptin, an adipokine overexpressed in obese patients, has been investigated in studies on breast carcinogenesis, but its effects on oxidative stress remain largely unexplored, especially in breast cancer. The study used three human mammary epithelial cell models presenting different neoplastic status (healthy primary HMECs, neoplastic MCF-7 cells and neoplastic MDA-MB-231 cells) to determine the effects of leptin on short-term ROS production and to characterize the enzymes involved. All three cell models significantly expressed NADPH oxidase isoform 5 (NOX5) in our culture conditions. All models showed induced ROS production regardless of leptin concentration (10 ng/ml mimicking good health, 100 ng/ml mimicking obesity). Cell treatment with either siRNA against NOX5, NOX inhibitor DPI or a calcium channel blocker (verapamil) confirmed the putative involvement of the NOX5 isoenzyme in ROS production. Moreover, cell treatments suppressed ROS production under leptin at both concentrations. Neoplastic cells appeared unable to downregulate NOX5 mRNA expression under leptin. Leptin emerged as a potential activator of ROS production in human epithelial mammary cells, where the ROS production was apparently linked to NOX5 activation. This novel finding could shed light on the potential role of obesity-associated hyperleptinemia in mammary cells via the activation of NOX enzymes.
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Copy and paste a formatted citation
Spandidos Publications style
Mahbouli S, Der Vartanian A, Ortega S, Rougé S, Vasson M and Rossary A: Leptin induces ROS via NOX5 in healthy and neoplastic mammary epithelial cells. Oncol Rep 38: 3254-3264, 2017.
APA
Mahbouli, S., Der Vartanian, A., Ortega, S., Rougé, S., Vasson, M., & Rossary, A. (2017). Leptin induces ROS via NOX5 in healthy and neoplastic mammary epithelial cells. Oncology Reports, 38, 3254-3264. https://doi.org/10.3892/or.2017.6009
MLA
Mahbouli, S., Der Vartanian, A., Ortega, S., Rougé, S., Vasson, M., Rossary, A."Leptin induces ROS via NOX5 in healthy and neoplastic mammary epithelial cells". Oncology Reports 38.5 (2017): 3254-3264.
Chicago
Mahbouli, S., Der Vartanian, A., Ortega, S., Rougé, S., Vasson, M., Rossary, A."Leptin induces ROS via NOX5 in healthy and neoplastic mammary epithelial cells". Oncology Reports 38, no. 5 (2017): 3254-3264. https://doi.org/10.3892/or.2017.6009
Copy and paste a formatted citation
x
Spandidos Publications style
Mahbouli S, Der Vartanian A, Ortega S, Rougé S, Vasson M and Rossary A: Leptin induces ROS via NOX5 in healthy and neoplastic mammary epithelial cells. Oncol Rep 38: 3254-3264, 2017.
APA
Mahbouli, S., Der Vartanian, A., Ortega, S., Rougé, S., Vasson, M., & Rossary, A. (2017). Leptin induces ROS via NOX5 in healthy and neoplastic mammary epithelial cells. Oncology Reports, 38, 3254-3264. https://doi.org/10.3892/or.2017.6009
MLA
Mahbouli, S., Der Vartanian, A., Ortega, S., Rougé, S., Vasson, M., Rossary, A."Leptin induces ROS via NOX5 in healthy and neoplastic mammary epithelial cells". Oncology Reports 38.5 (2017): 3254-3264.
Chicago
Mahbouli, S., Der Vartanian, A., Ortega, S., Rougé, S., Vasson, M., Rossary, A."Leptin induces ROS via NOX5 in healthy and neoplastic mammary epithelial cells". Oncology Reports 38, no. 5 (2017): 3254-3264. https://doi.org/10.3892/or.2017.6009
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