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Article

A panel of markers for identification of malignant and non-malignant cells in culture from effusions

  • Authors:
    • Fabiana Pirani Carneiro
    • Maria Imaculada Muniz-Junqueira
    • Fabio Pittella-Silva
    • Marcos de Vasconcelos Carneiro
    • Gustavo Henrique Soares Takano
    • Leonora Maciel de Sousa Vianna
    • Luciano Barbosa De Andrade
    • Tercia Maria Mendes Lousa De Castro
    • Isabela Peres
    • Tatiana Karla Dos Santos Borges
    • Vânia Moraes Ferreira
    • Andrea Barretto Motoyama
  • View Affiliations / Copyright

    Affiliations: Pathological Anatomy Center, University Hospital of Brasilia, Brasília, DF 71966-700, Brazil, Pathology Department of Brasília University, Brasília, DF 71966-700, Brazil, Catholic University of Brasilia, Brasília, DF 71966-700, Brazil
  • Pages: 3538-3544
    |
    Published online on: October 11, 2017
       https://doi.org/10.3892/or.2017.6022
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Abstract

The aim of the present study was to identify cell types in primary culture from malignant and non-malignant effusions. Effusion samples were subjected to cytology and culture. Immunocytochemistry was performed in cytological slides to evaluate malignancy (positivity for malignancy markers) and in culture slides for identification of cell types in growth. A total of 143 effusion samples (pleural n=76; peritoneal n=37; pericardial n=4; and peritoneal lavage n=26) were analyzed. Cell growth was observed in 34.9% of all samples and immunocytochemistry for identification of cell types in culture slides was conclusive in 90% of them. In non-malignant samples (n=28), growth of mesothelial cells, macrophages and of both cell types was identified in 82.14, 10.71 and 7.14%, respectively. In malignant samples (n=17, all carcinomas), growth of malignant epithelial cells and of both malignant epithelial and mesothelial cells was identified in 41.17 and 23.52%, respectively. In the remaining 35.29% of malignant samples, the only cells in growth were mesothelial and/or macrophages instead of malignant epithelial cells. In conclusion, in culture of malignant effusions, mesothelial cells may be simultaneously identified with malignant epithelial cells. Besides, mesothelial cells and macrophages may be the only cells identified in malignant effusion culture. Therefore, a broad panel of cell markers should be used for unmistakable identification of cells in studies of effusion primary culture. The ideal malignant effusion sample to obtain culture of neoplastic cells should be that without the presence of mesothelial cells and macrophages.
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1 

Ruiz C, Kustermann S, Pietilae E, Vlajnic T, Baschiera B, Arabi L, Lorber T, Oeggerli M, Savic S, Obermann E, et al: Culture and drug profiling of patient derived malignant pleural effusions for personalized cancer medicine. PLoS One. 11:e01608072016. View Article : Google Scholar : PubMed/NCBI

2 

Roscilli G, De Vitis C, Ferrara FF, Noto A, Cherubini E, Ricci A, Mariotta S, Giarnieri E, Giovagnoli MR, Torrisi MR, et al: Human lung adenocarcinoma cell cultures derived from malignant pleural effusions as model system to predict patients chemosensitivity. J Transl Med. 14:612016. View Article : Google Scholar : PubMed/NCBI

3 

Bezdieniezhnykh N, Lykhova A, Semesiuk N, Okhrimenko R and Kudryavets Y: Establishment and characterization of new breast and ovarian cancer cell lines as a model for studying cellular plasticity in vitro. Exp Oncol. 38:94–100. 2016.PubMed/NCBI

4 

Ku JL, Park SC, Kim KH, Jeon YK, Kim SH, Shin YK, Noh DY, Im SA, Bang YJ, Han W, et al: Establishment and characterization of seven human breast cancer cell lines including two triple-negative cell lines. Int J Oncol. 43:2073–2081. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Basak SK, Veena MS, Oh S, Huang G, Srivatsan E, Huang M, Sharma S and Batra RK: The malignant pleural effusion as a model to investigate intratumoral heterogeneity in lung cancer. PLoS One. 4:e58842009. View Article : Google Scholar : PubMed/NCBI

6 

Ricci A, De Vitis C, Noto A, Fattore L, Mariotta S, Cherubini E, Roscilli G, Liguori G, Scognamiglio G, Rocco G, et al: TrkB is responsible for EMT transition in malignant pleural effusions derived cultures from adenocarcinoma of the lung. Cell Cycle. 12:1696–1703. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Chen SF, Lin YS, Jao SW, Chang YC, Liu CL, Lin YJ and Nieh S: Pulmonary adenocarcinoma in malignant pleural effusion enriches cancer stem cell properties during metastatic cascade. PLoS One. 8:e546592013. View Article : Google Scholar : PubMed/NCBI

8 

Yin T, Wang G, He S, Shen G, Su C, Zhang Y, Wei X, Ye T, Li L, Yang S, et al: Malignant pleural effusion and ascites induce epithelial-mesenchymal transition and cancer stem-like cell properties via the vascular endothelial growth factor (VEGF)/phosphatidylinositol 3-kinase (PI3K)/Akt/mechanistic target of eapamycin (mTOR) pathway. J Biol Chem. 291:26750–26761. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Francis IM, Alath P, George SS, Jaragh M, Al Jassar A and Kapila K: Metastatic breast carcinoma in pleural fluid: Correlation of receptor and HER2 status with the primary carcinoma - a pilot study. Diagn Cytopathol. 44:980–986. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Kalogeraki A, Lazopoulos G, Papadakis GZ, Tamiolakis D, Karvela-Kalogeraki I, Karvelas-Kalogerakis M, Segredakis J, Papadakis M, Moustou E, Datseri G, et al: Cytology of pericardial effusion due to malignancy. Rom J Intern Med. 54:179–183. 2016.PubMed/NCBI

11 

Kalogeraki A, Tamiolakis D, Datseri G, Lazopoulos G, Karvelas-Kalogerakis M, Segredakis J and Zoi I: Pleural effusion cytology due to malignancy. A combined cytomorphological-immunocytochemical study of 500 cases. Rev Port Pneumol 2006. 22:290–291. 2016.PubMed/NCBI

12 

Antonangelo L, Sales RK, Corá AP, Acencio MM, Teixeira LR and Vargas FS: Pleural fluid tumour markers in malignant pleural effusion with inconclusive cytologic results. Curr Oncol. 22:e336–e341. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Westfall DE, Fan X and Marchevsky AM: Evidence-based guidelines to optimize the selection of antibody panels in cytopathology: Pleural effusions with malignant epithelioid cells. Diagn Cytopathol. 38:9–14. 2010.PubMed/NCBI

14 

Su XY, Li GD, Liu WP, Xie B and Jiang YH: Cytological differential diagnosis among adenocarcinoma, epithelial mesothelioma, and reactive mesothelial cells in serous effusions by immunocytochemistry. Diagn Cytopathol. 39:900–908. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Lyons-Boudreaux V, Mody DR, Zhai J and Coffey D: Cytologic malignancy versus benignancy: How useful are the ‘newer’ markers in body fluid cytology? Arch Pathol Lab Med. 132:23–28. 2008.PubMed/NCBI

16 

Saleh HA, El-Fakharany M, Makki H, Kadhim A and Masood S: Differentiating reactive mesothelial cells from metastatic adenocarcinoma in serous effusions: The utility of immunocytochemical panel in the differential diagnosis. Diagn Cytopathol. 37:324–332. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Kim JH, Kim GE, Choi YD, Lee JS, Lee JH, Nam JH and Choi C: Immunocytochemical panel for distinguishing between adenocarcinomas and reactive mesothelial cells in effusion cell blocks. Diagn Cytopathol. 37:258–261. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Qureshi-Baig K, Ullmann P, Rodriguez F, Frasquilho S, Nazarov PV, Haan S and Letellier E: What do we learn from spheroid culture systems? Insights from tumorspheres derived from primary colon cancer tissue. PLoS One. 11:e01460522016. View Article : Google Scholar : PubMed/NCBI

19 

Yung S and Chan TM: Mesothelial cells. Perit Dial Int. 27 Suppl 2:S110–S115. 2007.PubMed/NCBI

20 

Yung S, Li FK and Chan TM: Peritoneal mesothelial cell culture and biology. Perit Dial Int. 26:162–173. 2006.PubMed/NCBI

21 

Mutsaers SE, Whitaker D and Papadimitriou JM: Stimulation of mesothelial cell proliferation by exudate macrophages enhances serosal wound healing in a murine model. Am J Pathol. 160:681–692. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Zhou Q and Yu X: Isolation and propagation of rat peritoneal mesothelial cells. Methods Mol Biol. 1397:25–34. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Ranieri D, Raffa S, Parente A, Del Monte Rossi S, Ziparo V and Torrisi MR: High adhesion of tumor cells to mesothelial monolayers derived from peritoneal wash of disseminated gastrointestinal cancers. PLoS One. 8:e576592013. View Article : Google Scholar : PubMed/NCBI

24 

Tsukada T, Fushida S, Harada S, Yagi Y, Kinoshita J, Oyama K, Tajima H, Fujita H, Ninomiya I, Fujimura T, et al: The role of human peritoneal mesothelial cells in the fibrosis and progression of gastric cancer. Int J Oncol. 41:476–482. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Cabourne EJ, Roberts G, Goldin R, Ryder T, Mobberly M and Ziprin P: Investigation of tumor-peritoneal interactions in the pathogenesis of peritoneal metastases using a novel ex vivo peritoneal model. J Surg Res. 164:e265–e272. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Lv M, Leng JH, Hao YY, Sun Y, Cha N and Wu GP: Expression and significance of MOC-31 and calretinin in pleural fluid of patients with lung cancer. Diagn Cytopathol. 43:527–531. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Hyun TS, Barnes M and Tabatabai ZL: The diagnostic utility of D2-40, calretinin, CK5/6, desmin and MOC-31 in the differentiation of mesothelioma from adenocarcinoma in pleural effusion cytology. Acta Cytol. 56:527–532. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Fetsch PA, Simsir A and Abati A: Comparison of antibodies to HBME-1 and calretinin for the detection of mesothelial cells in effusion cytology. Diagn Cytopathol. 25:158–161. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Zhao L, Guo M, Sneige N and Gong Y: Value of PAX8 and WT1 immunostaining in confirming the ovarian origin of metastatic carcinoma in serous effusion specimens. Am J Clin Pathol. 137:304–309. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Oda T, Ogata S, Kawaguchi S, Minabe S, Dokyu M, Takahashi H, Kumazawa F, Shimazaki H, Takano M, Hase K, et al: Immunocytochemical utility of claudin-4 versus those of Ber-EP4 and MOC-31 in effusion cytology. Diagn Cytopathol. 44:499–504. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Kundu UR and Krishnamurthy S: Use of the monoclonal antibody MOC-31 as an immunomarker for detecting metastatic adenocarcinoma in effusion cytology. Cancer Cytopathol. 119:272–278. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Kim NI, Kim GE and Lee JS: Diagnostic usefulness of claudin-3 and claudin-4 for immunocytochemical differentiation between metastatic adenocarcinoma cells and reactive mesothelial cells in effusion cell blocks. Acta Cytol. 60:232–239. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Ikeda K, Tate G, Suzuki T, Kitamura T and Mitsuya T: IMP3/L523S, a novel immunocytochemical marker that distinguishes benign and malignant cells: The expression profiles of IMP3/L523S in effusion cytology. Hum Pathol. 41:745–750. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Hanley KZ, Facik MS, Bourne PA, Yang Q, Spaulding BO, Bonfiglio TA and Xu H: Utility of anti-L523S antibody in the diagnosis of benign and malignant serous effusions. Cancer. 114:49–56. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Jing X, Li QK, Bedrossian U and Michael CW: Morphologic and immunocytochemical performances of effusion cell blocks prepared using 3 different methods. Am J Clin Pathol. 139:177–182. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Ebata T, Okuma Y, Nakahara Y, Yomota M, Takagi Y, Hosomi Y, Asami E, Omuro Y, Hishima T, Okamura T, et al: Retrospective analysis of unknown primary cancers with malignant pleural effusion at initial diagnosis. Thorac Cancer. 7:39–43. 2016. View Article : Google Scholar : PubMed/NCBI

37 

Lew M, Pang JC, Jing X, Fields KL and Roh MH: Young investigator challenge: The utility of GATA3 immunohistochemistry in the evaluation of metastatic breast carcinomas in malignant effusions. Cancer Cytopathol. 123:576–581. 2015. View Article : Google Scholar : PubMed/NCBI

38 

Porcel JM, Palma R, Bielsa S, Esquerda A, Gatius S, Matias-Guiu X and Salud A: TTF-1 and napsin A on cell blocks and supernatants of pleural fluids for labeling malignant effusions. Respirology. 20:831–833. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Moh M, Krings G, Ates D, Aysal A, Kim GE and Rabban JT: SATB2 expression distinguishes ovarian metastases of colorectal and appendiceal origin from primary ovarian Tumors of mucinous or endometrioid type. Am J Surg Pathol. 40:419–432. 2016. View Article : Google Scholar : PubMed/NCBI

40 

Seipel AH, Samaratunga H, Delahunt B, Wiklund P, Clements M and Egevad L: Immunohistochemistry of ductal adenocarcinoma of the prostate and adenocarcinomas of non-prostatic origin: A comparative study. APMIS. 124:263–270. 2016. View Article : Google Scholar : PubMed/NCBI

41 

McKnight R, Cohen C and Siddiqui MT: Utility of paired box gene 8 (PAX8) expression in fluid and fine-needle aspiration cytology: an immunohistochemical study of metastatic ovarian serous carcinoma. Cancer Cytopathol. 25:298–302. 2010. View Article : Google Scholar

42 

Waters L, Crumley S, Truong L, Mody D and Coffey D: PAX2 and PAX8: Useful markers for metastatic effusions. Acta Cytol. 58:60–66. 2014. View Article : Google Scholar : PubMed/NCBI

43 

Barr ML, Jilaveanu LB, Camp RL, Adeniran AJ, Kluger HM and Shuch B: PAX-8 expression in renal tumours and distant sites: A useful marker of primary and metastatic renal cell carcinoma? J Clin Pathol. 68:12–17. 2015. View Article : Google Scholar : PubMed/NCBI

44 

Chute DJ, Kong CS and Stelow EB: Immunohistochemistry for the detection of renal cell carcinoma in effusion cytology. Diagn Cytopathol. 39:118–123. 2011. View Article : Google Scholar : PubMed/NCBI

45 

Karabork A, Kaygusuz G and Ekinci C: The best immunohisto-chemical panel for differentiating hepatocellular carcinoma from metastatic adenocarcinoma. Pathol Res Pract. 206:572–577. 2010. View Article : Google Scholar : PubMed/NCBI

46 

Pu RT, Pang Y and Michael CW: Utility of WT-1, p63, MOC31, mesothelin, and cytokeratin (K903 and CK5/6) immunostains in differentiating adenocarcinoma, squamous cell carcinoma, and malignant mesothelioma in effusions. Diagn Cytopathol. 36:20–25. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Carneiro FP, Muniz-Junqueira MI, Pittella-Silva F, Carneiro M, Takano GH, Vianna LM, De Andrade LB, De Castro TM, Peres I, Dos Santos Borges TK, Dos Santos Borges TK, et al: A panel of markers for identification of malignant and non-malignant cells in culture from effusions. Oncol Rep 38: 3538-3544, 2017.
APA
Carneiro, F.P., Muniz-Junqueira, M.I., Pittella-Silva, F., Carneiro, M., Takano, G.H., Vianna, L.M. ... Motoyama, A.B. (2017). A panel of markers for identification of malignant and non-malignant cells in culture from effusions. Oncology Reports, 38, 3538-3544. https://doi.org/10.3892/or.2017.6022
MLA
Carneiro, F. P., Muniz-Junqueira, M. I., Pittella-Silva, F., Carneiro, M., Takano, G. H., Vianna, L. M., De Andrade, L. B., De Castro, T. M., Peres, I., Dos Santos Borges, T. K., Ferreira, V. M., Motoyama, A. B."A panel of markers for identification of malignant and non-malignant cells in culture from effusions". Oncology Reports 38.6 (2017): 3538-3544.
Chicago
Carneiro, F. P., Muniz-Junqueira, M. I., Pittella-Silva, F., Carneiro, M., Takano, G. H., Vianna, L. M., De Andrade, L. B., De Castro, T. M., Peres, I., Dos Santos Borges, T. K., Ferreira, V. M., Motoyama, A. B."A panel of markers for identification of malignant and non-malignant cells in culture from effusions". Oncology Reports 38, no. 6 (2017): 3538-3544. https://doi.org/10.3892/or.2017.6022
Copy and paste a formatted citation
x
Spandidos Publications style
Carneiro FP, Muniz-Junqueira MI, Pittella-Silva F, Carneiro M, Takano GH, Vianna LM, De Andrade LB, De Castro TM, Peres I, Dos Santos Borges TK, Dos Santos Borges TK, et al: A panel of markers for identification of malignant and non-malignant cells in culture from effusions. Oncol Rep 38: 3538-3544, 2017.
APA
Carneiro, F.P., Muniz-Junqueira, M.I., Pittella-Silva, F., Carneiro, M., Takano, G.H., Vianna, L.M. ... Motoyama, A.B. (2017). A panel of markers for identification of malignant and non-malignant cells in culture from effusions. Oncology Reports, 38, 3538-3544. https://doi.org/10.3892/or.2017.6022
MLA
Carneiro, F. P., Muniz-Junqueira, M. I., Pittella-Silva, F., Carneiro, M., Takano, G. H., Vianna, L. M., De Andrade, L. B., De Castro, T. M., Peres, I., Dos Santos Borges, T. K., Ferreira, V. M., Motoyama, A. B."A panel of markers for identification of malignant and non-malignant cells in culture from effusions". Oncology Reports 38.6 (2017): 3538-3544.
Chicago
Carneiro, F. P., Muniz-Junqueira, M. I., Pittella-Silva, F., Carneiro, M., Takano, G. H., Vianna, L. M., De Andrade, L. B., De Castro, T. M., Peres, I., Dos Santos Borges, T. K., Ferreira, V. M., Motoyama, A. B."A panel of markers for identification of malignant and non-malignant cells in culture from effusions". Oncology Reports 38, no. 6 (2017): 3538-3544. https://doi.org/10.3892/or.2017.6022
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