Knockdown of HIP1 expression promotes ligand‑induced endocytosis of EGFR in HeLa cells

Li,Dan; Chen,Fenglin; Ding,Jian; Lin,Na; Li,Zonghai; Wang,Xiaozhong

doi:10.3892/or.2017.6025

December-2017 Volume 38 Issue 6

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December-2017 Volume 38 Issue 6

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Article Open Access

Knockdown of HIP1 expression promotes ligand‑induced endocytosis of EGFR in HeLa cells

Authors:
- Dan Li
- Fenglin Chen
- Jian Ding
- Na Lin
- Zonghai Li
- Xiaozhong Wang
View Affiliations / Copyright

Affiliations: Department of Gastroenterology, Union Hospital of Fujian Medical University, Gulou, Fuzhou, Fujian 350001, P.R. China, Department of Gastroenterology, The First Affiliated Hospital of Fujian Medical University, Taijiang, Fuzhou, Fujian 350005, P.R. China, State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University School of Medicine, Shanghai 200032, P.R. China

Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 3387-3391
|
Published online on: October 12, 2017

https://doi.org/10.3892/or.2017.6025
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Abstract

Huntington-interacting protein 1 (HIP1) is associated with various tumor types; however, its precise functions in tumor cells are unclear. In this study, the effects of HIP1 on the degradation of EGFR, which have important roles in carcinogenesis after EGF stimulation, were examined. After screening 17 cell lines, the coexpression of HIP1 and EGFR was detected in HeLa cells. Accordingly, the expression of HIP1 was knocked down in HeLa cells using various HIP1 siRNA sequences. The endocytosis of EGFR and localization of clathrin in HeLa cells were examined after stimulation by EGF at various concentrations (i.e., 1.5 and 100 ng/ml). After HIP1 expression was blocked by siRNAs, EGFR endocytosis was accelerated and this effect was dependent on the EGF concentration. This endocytosis was colocalized with clathrin expression. These findings indicate that the inhibition of HIP1 can accelerate the endocytosis and degradation of EGFR. Furthermore, they suggest that HIP1 is a potential therapeutic target for various cancer types, particularly those with high EGFR expression, but further research is needed to examine this hypothesis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Knockdown of HIP1 expression promotes ligand‑induced endocytosis of EGFR in HeLa cells

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