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Article

Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway

  • Authors:
    • Xiao-Qing Tian
    • Fang-Fang Guo
    • Dan-Feng Sun
    • Ying-Chao Wang
    • Li Yang
    • Sheng-Liang Chen
    • Jie Hong
    • Jing-Yuan Fang
  • View Affiliations / Copyright

    Affiliations: Division of Gastroenterology and Hepatology, Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, State Key Laboratory for Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, Shanghai 200001, P.R. China
  • Pages: 3685-3692
    |
    Published online on: October 12, 2017
       https://doi.org/10.3892/or.2017.6031
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Abstract

Zinc finger protein 278 is a zinc finger transcription factor encoded on the 22q12.2 chromosome. Previous studies revealed that ZNF278 expression was significantly upregulated in colorectal cancer (CRC) tissue compared to adjacent non-tumor tissue. However, the expression and specific roles of ZNF278 in CRC remain unknown. ZNF278 expression was knocked down using specific siRNAs, which was confirmed by western blotting, and the effects of ZNF278 siRNAs on CRC cell proliferation were investigated. In addition, the effects of ZNF278 overexpression were confirmed by western blotting and cell proliferation assay. Correlations between ZNF278 and the ERK/MAPK pathway were also detected by western blotting. We found that ZNF278 knockdown significantly induced cell cycle arrest, resulting in cyclin D1/E1 downregulation and p21 upregulation. Moreover, we demonstrated that downregulation of ZNF278 decreased the proliferation of CRC cells via inhibition of the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway for the first time. In conclusion, ZNF278 played a prominent role in the pathogenesis of CRC, and promoted CRC cell proliferation via the ERK/MAPK pathway, suggesting that it may act as a potential target in the diagnosis or treatment of CRC.
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1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Center MM, Jemal A and Ward E: International trends in colorectal cancer incidence rates. Cancer Epidemiol Biomarkers Prev. 18:1688–1694. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Center MM, Jemal A, Smith RA and Ward E: Worldwide variations in colorectal cancer. CA Cancer J Clin. 59:366–378. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Pero R, Lembo F, Palmieri EA, Vitiello C, Fedele M, Fusco A, Bruni CB and Chiariotti L: PATZ attenuates the RNF4-mediated enhancement of androgen receptor-dependent transcription. J Biol Chem. 277:3280–3285. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Fedele M, Benvenuto G, Pero R, Majello B, Battista S, Lembo F, Vollono E, Day PM, Santoro M, Lania L, et al: A novel member of the BTB/POZ family, PATZ, associates with the RNF4 RING finger protein and acts as a transcriptional repressor. J Biol Chem. 275:7894–7901. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Mastrangelo T, Modena P, Tornielli S, Bullrich F, Testi MA, Mezzelani A, Radice P, Azzarelli A, Pilotti S, Croce CM, et al: A novel zinc finger gene is fused to EWS in small round cell tumor. Oncogene. 19:3799–3804. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Tian X, Sun D, Zhang Y, Zhao S, Xiong H and Fang J: Zinc finger protein 278, a potential oncogene in human colorectal cancer. Acta Biochim Biophys Sin. 40:289–296. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Lu R, Wang X, Chen ZF, Sun DF, Tian XQ and Fang JY: Inhibition of the extracellular signal-regulated kinase/mitogen-activated protein kinase pathway decreases DNA methylation in colon cancer cells. J Biol Chem. 282:12249–12259. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Ye X, Nalepa G, Welcker M, Kessler BM, Spooner E, Qin J, Elledge SJ, Clurman BE and Harper JW: Recognition of phosphodegron motifs in human cyclin E by the SCFFbw7 ubiquitin ligase. J Biol Chem. 279:50110–50119. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Sherr CJ: Cancer cell cycles. Science. 274:1672–1677. 1996. View Article : Google Scholar : PubMed/NCBI

11 

Roberts PJ and Der CJ: Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer. Oncogene. 26:3291–3310. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Hong SK, Yoon S, Moelling C, Arthan D and Park JI: Noncatalytic function of ERK1/2 can promote Raf/MEK/ERK-mediated growth arrest signaling. J Biol Chem. 284:33006–33018. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Crews CM, Alessandrini A and Erikson RL: The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science. 258:478–480. 1992. View Article : Google Scholar : PubMed/NCBI

14 

Cowley S, Paterson H, Kemp P and Marshall CJ: Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells. Cell. 77:841–852. 1994. View Article : Google Scholar : PubMed/NCBI

15 

Sung JJ, Lau JY, Goh KL and Leung WK: Asia Pacific Working Group on Colorectal Cancer: Increasing incidence of colorectal cancer in Asia: Implications for screening. Lancet Oncol. 6:871–876. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Fodde R: The APC gene in colorectal cancer. Eur J Cancer. 38:867–871. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Rochlitz CF, Herrmann R and de Kant E: Overexpression and amplification of c-myc during progression of human colorectal cancer. Oncology. 53:448–454. 1996. View Article : Google Scholar : PubMed/NCBI

18 

Abdollahi A, Pisarcik D, Roberts D, Weinstein J, Cairns P and Hamilton TC: LOT1 (PLAGL1/ZAC1), the candidate tumor suppressor gene at chromosome 6q24-25, is epigenetically regulated in cancer. J Biol Chem. 278:6041–6049. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Pourquié O: Developmental biology. A macho way to make muscles. Nature. 409:679–680. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Purandare SM, Ware SM, Kwan KM, Gebbia M, Bassi MT, Deng JM, Vogel H, Behringer RR, Belmont JW and Casey B: A complex syndrome of left-right axis, central nervous system and axial skeleton defects in Zic3 mutant mice. Development. 129:2293–2302. 2002.PubMed/NCBI

21 

Yang JJ: A novel zinc finger protein, ZZaPK, interacts with ZAK and stimulates the ZAK-expressing cells re-entering the cell cycle. Biochem Biophys Res Commun. 301:71–77. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Valentino T, Palmieri D, Vitiello M, Simeone A, Palma G, Arra C, Chieffi P, Chiariotti L, Fusco A and Fedele M: Embryonic defects and growth alteration in mice with homozygous disruption of the Patz1 gene. J Cell Physiol. 228:646–653. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Chieffi P and Chieffi S: Molecular biomarkers as potential targets for therapeutic strategies in human testicular germ cell tumors: An overview. J Cell Physiol. 228:1641–1646. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Chiappetta G, Valentino T, Vitiello M, Pasquinelli R, Monaco M, Palma G, Sepe R, Luciano A, Pallante P, Palmieri D, et al: PATZ1 acts as a tumor suppressor in thyroid cancer via targeting p53-dependent genes involved in EMT and cell migration. Oncotarget. 6:5310–5323. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Ow JR, Ma H, Jean A, Goh Z, Lee YH, Chong YM, Soong R, Fu XY, Yang H and Wu Q: Patz1 regulates embryonic stem cell identity. Stem Cells Dev. 23:1062–1073. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Tritz R, Mueller BM, Hickey MJ, Lin AH, Gomez GG, Hadwiger P, Sah DW, Muldoon L, Neuwelt EA and Kruse CA: siRNA Down-regulation of the PATZ1 gene in human glioma cells increases their sensitivity to apoptotic stimuli. Cancer Ther. 6:865–876. 2008.PubMed/NCBI

27 

Pero R, Palmieri D, Angrisano T, Valentino T, Federico A, Franco R, Lembo F, Klein-Szanto AJ, Del Vecchio L, Montanaro D, et al: POZ-, AT-hook-, and zinc finger-containing protein (PATZ) interacts with human oncogene B cell lymphoma 6 (BCL6) and is required for its negative autoregulation. J Biol Chem. 287:18308–18317. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Keskin N, Deniz E, Eryilmaz J, Un M, Batur T, Ersahin T, Atalay Cetin R, Sakaguchi S, Ellmeier W and Erman B: PATZ1 is a DNA damage-responsive transcription factor that inhibits p53 function. Mol Cell Biol. 35:1741–1753. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Valentino T, Palmieri D, Vitiello M, Pierantoni GM, Fusco A and Fedele M: PATZ1 interacts with p53 and regulates expression of p53-target genes enhancing apoptosis or cell survival based on the cellular context. Cell Death Dis. 4:e9632013. View Article : Google Scholar : PubMed/NCBI

30 

Cho JH, Kim MJ, Kim KJ and Kim JR: POZ/BTB and AT-hook-containing zinc finger protein 1 (PATZ1) inhibits endothelial cell senescence through a p53 dependent pathway. Cell Death Differ. 19:703–712. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Yan L, Gu H, Li J, Xu M, Liu T, Shen Y, Chen B and Zhang G: RKIP and 14-3-3ε exert an opposite effect on human gastric cancer cells SGC7901 by regulating the ERK/MAPK pathway differently. Dig Dis Sci. 58:389–396. 2013. View Article : Google Scholar : PubMed/NCBI

32 

De Luca A, Maiello MR, D'Alessio A, Pergameno M and Normanno N: The RAS/RAF/MEK/ERK and the PI3K/AKT signalling pathways: Role in cancer pathogenesis and implications for therapeutic approaches. Expert Opin Ther Targets. 16 Suppl 2:S17–S27. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Levidou G, Saetta AA, Gigelou F, Karlou M, Papanastasiou P, Stamatelli A, Kavantzas N, Michalopoulos NV, Agrogiannis G, Patsouris E, et al: ERK/pERK expression and B-raf mutations in colon adenocarcinomas: Correlation with clinicopathological characteristics. World J Surg Oncol. 10:472012. View Article : Google Scholar : PubMed/NCBI

34 

Santarpia L, Lippman SM and El-Naggar AK: Targeting the MAPK-RAS-RAF signaling pathway in cancer therapy. Expert Opin Ther Targets. 16:103–119. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Fang JY and Richardson BC: The MAPK signalling pathways and colorectal cancer. Lancet Oncol. 6:322–327. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tian X, Guo F, Sun D, Wang Y, Yang L, Chen S, Hong J and Fang J: Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway. Oncol Rep 38: 3685-3692, 2017.
APA
Tian, X., Guo, F., Sun, D., Wang, Y., Yang, L., Chen, S. ... Fang, J. (2017). Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway. Oncology Reports, 38, 3685-3692. https://doi.org/10.3892/or.2017.6031
MLA
Tian, X., Guo, F., Sun, D., Wang, Y., Yang, L., Chen, S., Hong, J., Fang, J."Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway". Oncology Reports 38.6 (2017): 3685-3692.
Chicago
Tian, X., Guo, F., Sun, D., Wang, Y., Yang, L., Chen, S., Hong, J., Fang, J."Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway". Oncology Reports 38, no. 6 (2017): 3685-3692. https://doi.org/10.3892/or.2017.6031
Copy and paste a formatted citation
x
Spandidos Publications style
Tian X, Guo F, Sun D, Wang Y, Yang L, Chen S, Hong J and Fang J: Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway. Oncol Rep 38: 3685-3692, 2017.
APA
Tian, X., Guo, F., Sun, D., Wang, Y., Yang, L., Chen, S. ... Fang, J. (2017). Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway. Oncology Reports, 38, 3685-3692. https://doi.org/10.3892/or.2017.6031
MLA
Tian, X., Guo, F., Sun, D., Wang, Y., Yang, L., Chen, S., Hong, J., Fang, J."Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway". Oncology Reports 38.6 (2017): 3685-3692.
Chicago
Tian, X., Guo, F., Sun, D., Wang, Y., Yang, L., Chen, S., Hong, J., Fang, J."Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway". Oncology Reports 38, no. 6 (2017): 3685-3692. https://doi.org/10.3892/or.2017.6031
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