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Article

SPLUNC1 knockout enhances LPS-induced lung injury by increasing recruitment of CD11b+Gr-1+ cells to the spleen of mice

  • Authors:
    • Han Zhang
    • Xiaoling Li
    • Shan Liao
    • Heran Wang
    • Pan Chen
    • Guangchao Zhu
    • Yanwei Luo
    • Guiyuan Li
    • Yanhong Zhou
  • View Affiliations / Copyright

    Affiliations: The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan 410078, P.R. China, Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China
  • Pages: 358-366
    |
    Published online on: November 1, 2017
       https://doi.org/10.3892/or.2017.6063
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Abstract

Short palate, lung and nasal epithelium clone 1 (SPLUNC1) is a tissue-specific gene of nasopharyngeal tissue, and has been recognized as a potential tumor-suppressor gene in nasopharyngeal carcinoma. As a secreted protein, SPLUNC1 plays an important role in innate immunity including antimicrobial and host defense. However, the related immune cells which are regulated by SPLUNC1 remain elusive. In the present study, an acute lung injury (ALI) mouse model was established by administration of lipopolysaccharide (LPS) intraperitoneal injections to wild-type and SPLUNC1-/- mice (5 mg/kg). Pathologic results showed that the SPLUNC1-/- group appeared to have more severe pulmonary damage and infiltrated inflammatory cells compared with the WT group after LPS treatment for 24, 48, 72 and 96 h. The mRNA expression levels of interleukin-6 (IL-6), chemokine (C-C motif) ligand-2 (CCL-2), chemokine (C-C motif) ligand-3 (CCL-3) and chemokine (C-X-C motif) ligand-1 (CXCL-1) in lungs of the SPLUNC1-/- group were higher than these levels in lungs of the WT group at different time points after LPS injection. The percentage of splenic CD11b+Gr-1+ myeloid-derived suppressor cells (MDSCs) in the SPLUNC1-/- mice was higher than this percentage in the WT mice at the time points of 72 and 96 h post LPS injection (P<0.05). These findings demonstrated that SPLUNC1 had a certain protective effect on the LPS-induced ALI mouse model as well as it was found to inhibit the recruitment of MDSCs to the spleen in this model.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Li X, Liao S, Wang H, Chen P, Zhu G, Luo Y, Li G and Zhou Y: SPLUNC1 knockout enhances LPS-induced lung injury by increasing recruitment of CD11b+Gr-1+ cells to the spleen of mice. Oncol Rep 39: 358-366, 2018.
APA
Zhang, H., Li, X., Liao, S., Wang, H., Chen, P., Zhu, G. ... Zhou, Y. (2018). SPLUNC1 knockout enhances LPS-induced lung injury by increasing recruitment of CD11b+Gr-1+ cells to the spleen of mice. Oncology Reports, 39, 358-366. https://doi.org/10.3892/or.2017.6063
MLA
Zhang, H., Li, X., Liao, S., Wang, H., Chen, P., Zhu, G., Luo, Y., Li, G., Zhou, Y."SPLUNC1 knockout enhances LPS-induced lung injury by increasing recruitment of CD11b+Gr-1+ cells to the spleen of mice". Oncology Reports 39.1 (2018): 358-366.
Chicago
Zhang, H., Li, X., Liao, S., Wang, H., Chen, P., Zhu, G., Luo, Y., Li, G., Zhou, Y."SPLUNC1 knockout enhances LPS-induced lung injury by increasing recruitment of CD11b+Gr-1+ cells to the spleen of mice". Oncology Reports 39, no. 1 (2018): 358-366. https://doi.org/10.3892/or.2017.6063
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Li X, Liao S, Wang H, Chen P, Zhu G, Luo Y, Li G and Zhou Y: SPLUNC1 knockout enhances LPS-induced lung injury by increasing recruitment of CD11b+Gr-1+ cells to the spleen of mice. Oncol Rep 39: 358-366, 2018.
APA
Zhang, H., Li, X., Liao, S., Wang, H., Chen, P., Zhu, G. ... Zhou, Y. (2018). SPLUNC1 knockout enhances LPS-induced lung injury by increasing recruitment of CD11b+Gr-1+ cells to the spleen of mice. Oncology Reports, 39, 358-366. https://doi.org/10.3892/or.2017.6063
MLA
Zhang, H., Li, X., Liao, S., Wang, H., Chen, P., Zhu, G., Luo, Y., Li, G., Zhou, Y."SPLUNC1 knockout enhances LPS-induced lung injury by increasing recruitment of CD11b+Gr-1+ cells to the spleen of mice". Oncology Reports 39.1 (2018): 358-366.
Chicago
Zhang, H., Li, X., Liao, S., Wang, H., Chen, P., Zhu, G., Luo, Y., Li, G., Zhou, Y."SPLUNC1 knockout enhances LPS-induced lung injury by increasing recruitment of CD11b+Gr-1+ cells to the spleen of mice". Oncology Reports 39, no. 1 (2018): 358-366. https://doi.org/10.3892/or.2017.6063
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