Influence of miR-7a and miR-24-3p on the SOX18 transcript in lung adenocarcinoma

  • Authors:
    • Mateusz Olbromski
    • Adam Rzechonek
    • Jedrzej Grzegrzolka
    • Natalia Glatzel-Plucinska
    • Angelika Chachaj
    • Bozena Werynska
    • Marzenna Podhorska-Okolow
    • Piotr Dziegiel
  • View Affiliations

  • Published online on: November 6, 2017     https://doi.org/10.3892/or.2017.6077
  • Pages: 201-208
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The molecular pathogenesis of the development of non-small cell lung carcinomas (NSCLCs) is extremely complex. Understanding the molecular basis of the development of this malignant tumor may enable the use of targeted therapy, which may result in a better treatment outome for these patients. Adenocarcinoma (AC) is the most common NSCLC subtype, equally common among smokers and non-smokers, and its pathogenesis remains unknown. The SOX18 protein is an important protein that plays a role in the development of blood and lymphatic vessels during the process of embryogenesis. Recent studies have also shown that the SOX18 protein may play a significant role in tumors, including lung cancers. In the present study, we analyzed the expression of the SOX18 protein and the mRNA level in postoperative samples of AC and non-malignant lung tissues (NMLTs), and a disparity in both levels was observed. Based on our previous observations that miR-7a and miR-24-3p are able to modulate SOX18 expression in NSCLC, the main aim of this study was to verify the miRNA modulation of the SOX18 transcript with the use of the MirTrap System in established lung cancer cell lines NCI-H1703, NCI-H522 and A549. The SOX18 mRNA expression level was significantly lower in AC than that noted in the NMLTs (P<0.0001). However, the protein levels were higher in AC cases compared to levels noted in the NMLTs (P<0.0001). Additionally, correlations between the RQ values of SOX18 in NMLT and AC cases (r=0.8195, P=0.0001), and between miR-7a and miR24-3p in AC cases (r=0.4344, P=0.0016), were noted. In conclusion, we confirmed that miR-7a and miR-24-3p are more highly expressed in NMLTs than in the AC samples, and that they modulate the SOX18 transcript in NSCLC cells.
View Figures
View References

Related Articles

Journal Cover

January-2018
Volume 39 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Olbromski M, Rzechonek A, Grzegrzolka J, Glatzel-Plucinska N, Chachaj A, Werynska B, Podhorska-Okolow M and Dziegiel P: Influence of miR-7a and miR-24-3p on the SOX18 transcript in lung adenocarcinoma. Oncol Rep 39: 201-208, 2018
APA
Olbromski, M., Rzechonek, A., Grzegrzolka, J., Glatzel-Plucinska, N., Chachaj, A., Werynska, B. ... Dziegiel, P. (2018). Influence of miR-7a and miR-24-3p on the SOX18 transcript in lung adenocarcinoma. Oncology Reports, 39, 201-208. https://doi.org/10.3892/or.2017.6077
MLA
Olbromski, M., Rzechonek, A., Grzegrzolka, J., Glatzel-Plucinska, N., Chachaj, A., Werynska, B., Podhorska-Okolow, M., Dziegiel, P."Influence of miR-7a and miR-24-3p on the SOX18 transcript in lung adenocarcinoma". Oncology Reports 39.1 (2018): 201-208.
Chicago
Olbromski, M., Rzechonek, A., Grzegrzolka, J., Glatzel-Plucinska, N., Chachaj, A., Werynska, B., Podhorska-Okolow, M., Dziegiel, P."Influence of miR-7a and miR-24-3p on the SOX18 transcript in lung adenocarcinoma". Oncology Reports 39, no. 1 (2018): 201-208. https://doi.org/10.3892/or.2017.6077