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PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells

  • Authors:
    • Weidong Liu
    • Xuxu Liu
    • Lei Wang
    • Bin Zhu
    • Chang Zhang
    • Wei Jia
    • Hecheng Zhu
    • Xingdong Liu
    • Meizuo Zhong
    • Dan Xie
    • Yanyu Liu
    • Shasha Li
    • Jia Shi
    • Jianxing Lin
    • Xiaomeng Xia
    • Xingjun Jiang
    • Caiping Ren
  • View Affiliations / Copyright

    Affiliations: The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Changsha Kexin Cancer Hospital, Changsha, Hunan 410205, P.R. China, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Hunan 510060, P.R. China, Department of Gynecology and Obstetrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 45-52
    |
    Published online on: November 6, 2017
       https://doi.org/10.3892/or.2017.6080
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Abstract

Phospholipase C (PLC) is a pivotal enzyme in the phosphoinositide pathway that promotes the second messengers, diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), to participate in eukaryotic signal transduction. Several PLC isozymes are associated with cancer, such as PLC-β1, PLC-δ1, PLC-ε and PLC-γ1. However, the role of PLC-δ3 (PLCD3) in nasopharyngeal carcinoma (NPC) has not been investigated to date. In our previous study, we demonstrated that flotillin2 (Flot2) plays a pro-neoplastic role in NPC and is involved in tumour progression and metastasis. In the present study, we screened the interacting proteins of Flot2 using the yeast two-hybrid (Y2H) method and verified the interaction between PLCD3 and Flot2 by co-immunoprecipitation. We also investigated the biological functions of PLCD3 in NPC. Inhibition of PLCD3 expression impaired the malignant potential of 5-8F, a highly metastatic NPC cell line, by restraining its growth, proliferation, mobility and migration. The present study demonstrated that PLCD3 may be an oncogenic protein in NPC and that it plays an important role in the progression of NPC partially by interacting with Flot2.
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Copy and paste a formatted citation
Spandidos Publications style
Liu W, Liu X, Wang L, Zhu B, Zhang C, Jia W, Zhu H, Liu X, Zhong M, Xie D, Xie D, et al: PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells. Oncol Rep 39: 45-52, 2018.
APA
Liu, W., Liu, X., Wang, L., Zhu, B., Zhang, C., Jia, W. ... Ren, C. (2018). PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells. Oncology Reports, 39, 45-52. https://doi.org/10.3892/or.2017.6080
MLA
Liu, W., Liu, X., Wang, L., Zhu, B., Zhang, C., Jia, W., Zhu, H., Liu, X., Zhong, M., Xie, D., Liu, Y., Li, S., Shi, J., Lin, J., Xia, X., Jiang, X., Ren, C."PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells". Oncology Reports 39.1 (2018): 45-52.
Chicago
Liu, W., Liu, X., Wang, L., Zhu, B., Zhang, C., Jia, W., Zhu, H., Liu, X., Zhong, M., Xie, D., Liu, Y., Li, S., Shi, J., Lin, J., Xia, X., Jiang, X., Ren, C."PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells". Oncology Reports 39, no. 1 (2018): 45-52. https://doi.org/10.3892/or.2017.6080
Copy and paste a formatted citation
x
Spandidos Publications style
Liu W, Liu X, Wang L, Zhu B, Zhang C, Jia W, Zhu H, Liu X, Zhong M, Xie D, Xie D, et al: PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells. Oncol Rep 39: 45-52, 2018.
APA
Liu, W., Liu, X., Wang, L., Zhu, B., Zhang, C., Jia, W. ... Ren, C. (2018). PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells. Oncology Reports, 39, 45-52. https://doi.org/10.3892/or.2017.6080
MLA
Liu, W., Liu, X., Wang, L., Zhu, B., Zhang, C., Jia, W., Zhu, H., Liu, X., Zhong, M., Xie, D., Liu, Y., Li, S., Shi, J., Lin, J., Xia, X., Jiang, X., Ren, C."PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells". Oncology Reports 39.1 (2018): 45-52.
Chicago
Liu, W., Liu, X., Wang, L., Zhu, B., Zhang, C., Jia, W., Zhu, H., Liu, X., Zhong, M., Xie, D., Liu, Y., Li, S., Shi, J., Lin, J., Xia, X., Jiang, X., Ren, C."PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells". Oncology Reports 39, no. 1 (2018): 45-52. https://doi.org/10.3892/or.2017.6080
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