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Article

Cullin 7 is a predictor of poor prognosis in breast cancer patients and is involved in the proliferation and invasion of breast cancer cells by regulating the cell cycle and microtubule stability

  • Authors:
    • Ni Qiu
    • Yufang He
    • Siming Zhang
    • Xinpeng Hu
    • Mingsheng Chen
    • Hongsheng Li
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China
  • Pages: 603-610
    |
    Published online on: November 21, 2017
       https://doi.org/10.3892/or.2017.6106
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Abstract

Cullin 7 is the ‘scaffold’ of the cullin-RING-based E3 ligases which catalyze the final step of the ubiquitination cascade in eukaryotic organisms. Although one study has reported the involvement of cullin 7 in the invasion and migration of breast cancer cells without further exploration of its mechanisms, the expression of cullin 7 in breast cancer tissues and its clinical significance have not been reported. The present study evaluated cullin 7 protein expression in malignant and benign breast tissues using immunochemistry, and further analyzed the association of positive cullin 7 expression in breast cancer tissues with clinicopathological characteristics of breast cancer patients. Cullin 7 expression was further silenced in breast cancer cells by siRNA and its functions and mechanisms in cell proliferation and invasion were investigated. The results showed that high cullin 7 expression was significantly correlated with pathological stage (P=0.013) and lymph node metastasis (P=0.022) of breast cancer. Patients with high cullin 7 expression had a shorter overall survival rate than those with low cullin 7 expression (P=0.037). Silencing of cullin 7 expression significantly inhibited the proliferation (P<0.05) and invasion (P<0.05), increased S1 phase (P<0.05), but decreased G2 phase (P<0.05) in MDA-MB-231 and BT549 cells. In addition, silencing of cullin 7 expression decreased cyclin A, but increased p21 protein expression. Moreover, silencing of cullin 7 expression altered the cell shape, caused disorder in microtubules, and increased the microtubule regeneration in MDA-MB-231 and BT549 cells. In conclusion, cullin 7 is overexpressed in breast cancer tissues which is associated with the development and prognosis of breast cancer. Cullin 7 is involved in the proliferation and invasion of breast cancer cells by regulating the cell cycle and microtubule stability.
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Copy and paste a formatted citation
Spandidos Publications style
Qiu N, He Y, Zhang S, Hu X, Chen M and Li H: Cullin 7 is a predictor of poor prognosis in breast cancer patients and is involved in the proliferation and invasion of breast cancer cells by regulating the cell cycle and microtubule stability. Oncol Rep 39: 603-610, 2018.
APA
Qiu, N., He, Y., Zhang, S., Hu, X., Chen, M., & Li, H. (2018). Cullin 7 is a predictor of poor prognosis in breast cancer patients and is involved in the proliferation and invasion of breast cancer cells by regulating the cell cycle and microtubule stability. Oncology Reports, 39, 603-610. https://doi.org/10.3892/or.2017.6106
MLA
Qiu, N., He, Y., Zhang, S., Hu, X., Chen, M., Li, H."Cullin 7 is a predictor of poor prognosis in breast cancer patients and is involved in the proliferation and invasion of breast cancer cells by regulating the cell cycle and microtubule stability". Oncology Reports 39.2 (2018): 603-610.
Chicago
Qiu, N., He, Y., Zhang, S., Hu, X., Chen, M., Li, H."Cullin 7 is a predictor of poor prognosis in breast cancer patients and is involved in the proliferation and invasion of breast cancer cells by regulating the cell cycle and microtubule stability". Oncology Reports 39, no. 2 (2018): 603-610. https://doi.org/10.3892/or.2017.6106
Copy and paste a formatted citation
x
Spandidos Publications style
Qiu N, He Y, Zhang S, Hu X, Chen M and Li H: Cullin 7 is a predictor of poor prognosis in breast cancer patients and is involved in the proliferation and invasion of breast cancer cells by regulating the cell cycle and microtubule stability. Oncol Rep 39: 603-610, 2018.
APA
Qiu, N., He, Y., Zhang, S., Hu, X., Chen, M., & Li, H. (2018). Cullin 7 is a predictor of poor prognosis in breast cancer patients and is involved in the proliferation and invasion of breast cancer cells by regulating the cell cycle and microtubule stability. Oncology Reports, 39, 603-610. https://doi.org/10.3892/or.2017.6106
MLA
Qiu, N., He, Y., Zhang, S., Hu, X., Chen, M., Li, H."Cullin 7 is a predictor of poor prognosis in breast cancer patients and is involved in the proliferation and invasion of breast cancer cells by regulating the cell cycle and microtubule stability". Oncology Reports 39.2 (2018): 603-610.
Chicago
Qiu, N., He, Y., Zhang, S., Hu, X., Chen, M., Li, H."Cullin 7 is a predictor of poor prognosis in breast cancer patients and is involved in the proliferation and invasion of breast cancer cells by regulating the cell cycle and microtubule stability". Oncology Reports 39, no. 2 (2018): 603-610. https://doi.org/10.3892/or.2017.6106
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