Open Access

miR‑328‑3p enhances the radiosensitivity of osteosarcoma and regulates apoptosis and cell viability via H2AX

  • Authors:
    • Zhen Yang
    • Qing-De Wa
    • Chao Lu
    • Wei Pan
    • Zi-Μo Lu
    • Jun Ao
  • View Affiliations

  • Published online on: November 27, 2017     https://doi.org/10.3892/or.2017.6112
  • Pages: 545-553
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteosarcoma is a kind of high-risk sarcoma of the skeleton typically observed in people under 25 years old. Currently, radiotherapy is widely applied in cancer treatment. However, osteosarcoma is radioresistant and accordingly new, more effective radiosensitizers are needed. miRNAs have been reported to play an important role in osteosarcoma radiosensitivity. We examined the modulating effect of miR‑328‑3p in vivo and in vitro. miR‑328‑3p was downregulated in HOS‑2R cells. The overexpression of miR‑328‑3p enhanced the radiosensitivity of osteosarcoma cells. miR‑328‑3p inhibited proliferation and promoted apoptosis in osteosarcoma cells under radiation conditions. In cells overexpressing miR‑328‑3p, H2AX expression was downregulated. We found that miR‑328‑3p targets H2AX and inhibits its expression. It was concluded, that miR‑328‑3p enhances the radiosensitization of osteosarcoma following X-ray irradiation, and determined that it directly targets H2AX to regulate radiosensitization.
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February-2018
Volume 39 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Yang Z, Wa Q, Lu C, Pan W, Lu Z and Ao J: miR‑328‑3p enhances the radiosensitivity of osteosarcoma and regulates apoptosis and cell viability via H2AX. Oncol Rep 39: 545-553, 2018
APA
Yang, Z., Wa, Q., Lu, C., Pan, W., Lu, Z., & Ao, J. (2018). miR‑328‑3p enhances the radiosensitivity of osteosarcoma and regulates apoptosis and cell viability via H2AX. Oncology Reports, 39, 545-553. https://doi.org/10.3892/or.2017.6112
MLA
Yang, Z., Wa, Q., Lu, C., Pan, W., Lu, Z., Ao, J."miR‑328‑3p enhances the radiosensitivity of osteosarcoma and regulates apoptosis and cell viability via H2AX". Oncology Reports 39.2 (2018): 545-553.
Chicago
Yang, Z., Wa, Q., Lu, C., Pan, W., Lu, Z., Ao, J."miR‑328‑3p enhances the radiosensitivity of osteosarcoma and regulates apoptosis and cell viability via H2AX". Oncology Reports 39, no. 2 (2018): 545-553. https://doi.org/10.3892/or.2017.6112