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Article

MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway

  • Authors:
    • Yanbing Wang
    • Shicai Sun
    • Jianting Xu
    • Yang Luo
    • Ying Xin
    • Xin Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Second Hospital of Jilin University, Changchun, P.R. China, Department of Orthopedics, Songyuan Integrated Traditional Chinese and Western Medicine Hospital, Jilin 130041, P.R. China, Cancer Center, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Orthopaedics, General Hospital of Chinese PLA, Beijing 100853, P.R. China, Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Jilin 130021, P.R. China
  • Pages: 589-596
    |
    Published online on: December 4, 2017
       https://doi.org/10.3892/or.2017.6129
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Abstract

We explored the function of microRNA-381 on osteosarcoma cell growth and anticancer mechanism for clinic treatment. MicroRNA-381 of osteosarcoma patients was decreased, microRNA-381 levels were more downregulated at stages III/IV than those at stage I/II in osteosarcoma patients. Downregulation of microRNA-381 using anti-microRNA-381 mimics increased cell proliferation, decreased LDH activity and apoptosis, and inhibited caspase‑3/9 activities, Bax/Bcl-2 and p53 protein expression in vitro osteosarcoma cells through upregulation of LRH-1/Wnt/β-catenin signaling pathway. Overexpression of microRNA-381 using microRNA-381 mimics inhibited cell proliferation, induced apoptosis and increased LDH activity, caspase‑3/9 activities, expression of Bax/Bcl-2 and p53 protein in osteosarcoma of in vitro model through downregulation of LRH-1/Wnt/β-catenin signaling pathway. Si‑LRH‑1 promoted the anticancer effects of microRNA-381 on osteosarcoma cell growth through Wnt/β‑catenin signaling pathway. Thus, our data suggested that microRNA-381 suppresses the proliferation of osteosarcoma cells through upregulation of LRH-1/Wnt/β-catenin signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Sun S, Xu J, Luo Y, Xin Y and Zhao X: MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway. Oncol Rep 39: 589-596, 2018.
APA
Wang, Y., Sun, S., Xu, J., Luo, Y., Xin, Y., & Zhao, X. (2018). MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway. Oncology Reports, 39, 589-596. https://doi.org/10.3892/or.2017.6129
MLA
Wang, Y., Sun, S., Xu, J., Luo, Y., Xin, Y., Zhao, X."MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway". Oncology Reports 39.2 (2018): 589-596.
Chicago
Wang, Y., Sun, S., Xu, J., Luo, Y., Xin, Y., Zhao, X."MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway". Oncology Reports 39, no. 2 (2018): 589-596. https://doi.org/10.3892/or.2017.6129
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Sun S, Xu J, Luo Y, Xin Y and Zhao X: MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway. Oncol Rep 39: 589-596, 2018.
APA
Wang, Y., Sun, S., Xu, J., Luo, Y., Xin, Y., & Zhao, X. (2018). MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway. Oncology Reports, 39, 589-596. https://doi.org/10.3892/or.2017.6129
MLA
Wang, Y., Sun, S., Xu, J., Luo, Y., Xin, Y., Zhao, X."MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway". Oncology Reports 39.2 (2018): 589-596.
Chicago
Wang, Y., Sun, S., Xu, J., Luo, Y., Xin, Y., Zhao, X."MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway". Oncology Reports 39, no. 2 (2018): 589-596. https://doi.org/10.3892/or.2017.6129
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