miR-141-3p suppresses proliferation and promotes apoptosis by targeting GLI2 in osteosarcoma cells Retraction in /10.3892/or.2023.8576

Wang,Nan; Li,Pengcheng; Liu,Wei; Wang,Ningning; Lu,Zhi; Feng,Jianzhou; Zeng,Xiandong; Yang,Jiujie; Wang,Yong; Zhao,Wei

doi:10.3892/or.2017.6150

February-2018 Volume 39 Issue 2

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February-2018 Volume 39 Issue 2

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Article

miR-141-3p suppresses proliferation and promotes apoptosis by targeting GLI2 in osteosarcoma cells

Retraction in: /10.3892/or.2023.8576

Authors:
- Nan Wang
- Pengcheng Li
- Wei Liu
- Ningning Wang
- Zhi Lu
- Jianzhou Feng
- Xiandong Zeng
- Jiujie Yang
- Yong Wang
- Wei Zhao
View Affiliations / Copyright

Affiliations: The 4th Department of Orthopedic Surgery, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, P.R. China, Department of Minimal Invasive Spinal Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, The 2nd Department of Cardiology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, P.R. China, Department of Nuclear Medicine, The 1st Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Surgical Oncology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, P.R. China
Pages: 747-754
|
Published online on: December 12, 2017

https://doi.org/10.3892/or.2017.6150
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Abstract

MicroRNAs (miRNAs) have been reported as key regulators in numerous diseases including osteosarcoma. The function of microRNA-141-3p (miR-141-3p) and whether this function is achieved by regulation of GLI family zinc finger 2 (GLI2) in osteosarcoma remain unclear. In the present study, we found decreased expression of miR-141-3p, but increased expression of GLI2 in osteosarcoma tissues and cell lines. In addition, we demonstrated a negative correlation between miR-141-3p and GLI2. Furthermore, we revealed that elevation of miR-141-3p resulted in a marked inhibition of proliferation and promotion of apoptosis as well as an obviously decrease in GLI2 in osteosarcoma cell lines. Furthermore, we determined that GLI2 is a target of miR-141-3p by a constructed luciferase assay. In addition, we showed that miR-141-3p could negatively regulate GLI2 and its downstream parathyroid hormone-related protein 1 (PTHRP1). Finally, through a series of antisense experiments we confirmed that the effect of miR-141-3p on proliferation and apoptosis was achieved through the GLI2 pathway in osteosarcoma cells. The findings of the present study may provide a new target for treating osteosarcoma.

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