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Article

Effects of epidermal growth factor receptor fusion protein on the cytotoxic activity of SOCS1-silenced dendritic cells in vitro

  • Authors:
    • Qichuan Jiang
    • Xuefeng Wang
    • Ming Qian
    • Dong Chen
    • Yanan Xu
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Otolaryngology Head and Neck Surgery, Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Health Sciences Center, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
  • Pages: 1306-1312
    |
    Published online on: December 15, 2017
       https://doi.org/10.3892/or.2017.6160
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Abstract

The aim of the present study was to observe the effects of cytokine signaling suppressor 1 (SOCS1)-silenced dendritic cells (DCs) pulsed with epidermal growth factor receptor (EGFR) fusion protein on the activation of T lymphocyte and cytotoxic T-lymphocyte (CTL) activity against Hep-2 cells. DCs were derived from the medullary cells of mice and authenticated by flow cytometry (FCM). Recombinant glutathione-S-transferase (GST)-EGFR fusion protein was produced and purified. After being pulsed with it, DCs were modified by recombinant SOCS1-siRNA adenoviral to silence SOCS1 gene expression. The maturation of DCs was evaluated by FCM. The effects of modified DCs on T-cell proliferation were assessed by MTT assay. The killing effects against Hep-2 cells of CTL were assessed by lactate dehydrogenase (LDH) release assay. High-purity DCs from the medullary cells of mice were obtained. Compared with the control, EGFR-pulsed DCs displayed higher expression of cell surface molecules, including CD83, CD860 and HLA-DR. The MTT assay revealed that all of the EGFR-pulsed, SOCS1‑silenced and EGFR-pulsed plus SOCS1-silenced DCs had an enhanced capacity to stimulate T-lymphocyte proliferation. As expected, EGFR-pulsed plus SOCS1-silenced DCs had the strongest effects on T-cell proliferation. The splenic T cells isolated from both EGFR-pulsed DC-immunized mice and EGFR-pulsed plus SOCS1-silenced DC-immunized mice enhanced the cytotoxicity against Hep-2 cells, while T cells from EGFR‑pulsed plus SOCS1-silenced DC-immunized mice exhibited significantly higher cytotoxicity than those from EGFR-DC-immunized mice. The EGFR-pulsed SOCS1‑siRNA-silenced DCs had the strongest effects on activation of T-cell proliferation and the CTL activity against Hep-2 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang Q, Wang X, Qian M, Chen D and Xu Y: Effects of epidermal growth factor receptor fusion protein on the cytotoxic activity of SOCS1-silenced dendritic cells in vitro. Oncol Rep 39: 1306-1312, 2018.
APA
Jiang, Q., Wang, X., Qian, M., Chen, D., & Xu, Y. (2018). Effects of epidermal growth factor receptor fusion protein on the cytotoxic activity of SOCS1-silenced dendritic cells in vitro. Oncology Reports, 39, 1306-1312. https://doi.org/10.3892/or.2017.6160
MLA
Jiang, Q., Wang, X., Qian, M., Chen, D., Xu, Y."Effects of epidermal growth factor receptor fusion protein on the cytotoxic activity of SOCS1-silenced dendritic cells in vitro". Oncology Reports 39.3 (2018): 1306-1312.
Chicago
Jiang, Q., Wang, X., Qian, M., Chen, D., Xu, Y."Effects of epidermal growth factor receptor fusion protein on the cytotoxic activity of SOCS1-silenced dendritic cells in vitro". Oncology Reports 39, no. 3 (2018): 1306-1312. https://doi.org/10.3892/or.2017.6160
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang Q, Wang X, Qian M, Chen D and Xu Y: Effects of epidermal growth factor receptor fusion protein on the cytotoxic activity of SOCS1-silenced dendritic cells in vitro. Oncol Rep 39: 1306-1312, 2018.
APA
Jiang, Q., Wang, X., Qian, M., Chen, D., & Xu, Y. (2018). Effects of epidermal growth factor receptor fusion protein on the cytotoxic activity of SOCS1-silenced dendritic cells in vitro. Oncology Reports, 39, 1306-1312. https://doi.org/10.3892/or.2017.6160
MLA
Jiang, Q., Wang, X., Qian, M., Chen, D., Xu, Y."Effects of epidermal growth factor receptor fusion protein on the cytotoxic activity of SOCS1-silenced dendritic cells in vitro". Oncology Reports 39.3 (2018): 1306-1312.
Chicago
Jiang, Q., Wang, X., Qian, M., Chen, D., Xu, Y."Effects of epidermal growth factor receptor fusion protein on the cytotoxic activity of SOCS1-silenced dendritic cells in vitro". Oncology Reports 39, no. 3 (2018): 1306-1312. https://doi.org/10.3892/or.2017.6160
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