Immune response-associated gene profiling in Japanese melanoma patients using multi-omics analysis

  • Authors:
    • Yasuto Akiyama
    • Yoshio Kiyohara
    • Shusuke Yoshikawa
    • Masaki Otsuka
    • Ryota Kondou
    • Chizu Nonomura
    • Haruo Miyata
    • Akira Iizuka
    • Tadashi Ashizawa
    • Keiichi Ohshima
    • Kenichi Urakami
    • Takeshi Nagashima
    • Masatoshi Kusuhara
    • Takashi Sugino
    • Ken Yamaguchi
  • View Affiliations

  • Published online on: December 21, 2017     https://doi.org/10.3892/or.2017.6173
  • Pages:1125-1131
Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Project High-tech Omics-based Patient Evaluation (HOPE), including comprehensive whole-exome sequencing (WES) and gene expression profiling (GEP) using freshly resected tumor specimens, has been in progress since its implementation in 2014. Among a total of 1,685 cancer patients, 13 melanoma patients were registered in the HOPE Project and were characterized using multi-omics analyses. Among the 13 melanoma patients, 4 were deceased, and 9 were alive. The mean overall survival (OS) and relapse‑free survival (RFS) times of the melanoma patients were 16.9 and 14.7 months, respectively. Previously, we developed an immune response‑associated gene list, which consisted of 164 genes in Project HOPE, for evaluating the immunological status. In the present study, the association of immune response‑associated gene expression with immunological parameters, such as programmed death-ligand 1 (PD-L1) and CD8 expression levels, single nucleotide variant (SNV) number, and Vogelstein driver gene mutation number, was investigated. With respect to PD-L1 expression, both immuno-suppression and immuno-stimulation-related genes were upregulated in PD-L1-positive melanomas. In contrast, regarding Vogelstein driver mutations, several T-cell activation-related genes were significantly downregulated in the high driver gene mutation group. In addition, many T-cell activation-related genes were upregulated in the CD8-positive melanomas. The correlation of immune response-associated gene expression with the survival time of the melanoma patients was investigated. Eight specific genes were commonly identified as genes that were significantly correlated for both the overall OS and RFS time, which could be possible prognostic factors for melanoma patients. These results revealed that an immune response-associated gene panel could be an informative tool for evaluating the immunological status prior to clinical immunotherapy in the upcoming era of genomic cancer medicine.

Related Articles

Journal Cover

March 2018
Volume 39 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Akiyama, Y., Kiyohara, Y., Yoshikawa, S., Otsuka, M., Kondou, R., Nonomura, C. ... Yamaguchi, K. (2018). Immune response-associated gene profiling in Japanese melanoma patients using multi-omics analysis. Oncology Reports, 39, 1125-1131. https://doi.org/10.3892/or.2017.6173
MLA
Akiyama, Y., Kiyohara, Y., Yoshikawa, S., Otsuka, M., Kondou, R., Nonomura, C., Miyata, H., Iizuka, A., Ashizawa, T., Ohshima, K., Urakami, K., Nagashima, T., Kusuhara, M., Sugino, T., Yamaguchi, K."Immune response-associated gene profiling in Japanese melanoma patients using multi-omics analysis". Oncology Reports 39.3 (2018): 1125-1131.
Chicago
Akiyama, Y., Kiyohara, Y., Yoshikawa, S., Otsuka, M., Kondou, R., Nonomura, C., Miyata, H., Iizuka, A., Ashizawa, T., Ohshima, K., Urakami, K., Nagashima, T., Kusuhara, M., Sugino, T., Yamaguchi, K."Immune response-associated gene profiling in Japanese melanoma patients using multi-omics analysis". Oncology Reports 39, no. 3 (2018): 1125-1131. https://doi.org/10.3892/or.2017.6173