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Article Open Access

Chronic oxymatrine treatment induces resistance and epithelial‑mesenchymal transition through targeting the long non-coding RNA MALAT1 in colorectal cancer cells

Retraction in: /10.3892/or.2024.8732
  • Authors:
    • Yibai Xiong
    • Jun Wang
    • Huirong Zhu
    • Lingshuang Liu
    • Yi Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Longhua Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200021, P.R. China, Department of Anorectal Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310000, P.R. China, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, P.R. China
    Copyright: © Xiong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 967-976
    |
    Published online on: January 10, 2018
       https://doi.org/10.3892/or.2018.6204
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Abstract

A major reason for colorectal cancer (CRC) chemoresistance is the enhanced migration and invasion of cancer cells, such as the cell acquisition of epithelial-mesenchymal transition (EMT). Long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been considered as a pro-oncogene in multiple cancers. However, the precise functional mechanism of lncRNA MALAT1 in chemoresistance and EMT is not well known. In the present study, we focused on the effect of oxymatrine on CRC cells and further investigated the role of MALAT1 in oxymatrine-induced resistance and EMT process. The human CRC cell line HT29 was exposed to increasing doses of oxymatrine to establish stable cell lines resistant to oxymatrine. The established HT29 oxymatrine resistant cells showed an EMT phenotype including specific morphologic changes, enhanced migratory and invasive capacity, and downregulation of E-cadherin protein expression. Subsequently, high-throughput HiSeq sequencing and RT-qPCR showed that lncRNA MALAT1 was significantly upregulated in the oxymatrine resistant cells (P<0.01), while knockdown of MALAT1 partially reversed the EMT phenotype in HT29 resistant cells. Furthermore, oxymatrine treatment suppressed the migration and invasion ability of CRC cells, however, this effect was significantly reversed by overexpression of MALAT1. Finally, we investigated the clinical role of MALAT1 and found that high lncRNA MALAT1 expression level is associated with poor prognosis in CRC patients receiving oxymatrine treatment (P<0.01). In conclusion, we demonstrate that lncRNA MALAT1 is a stimulator for oxymatrine resistance in CRC and it may provide therapeutic and prognostic information for CRC patients.
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Copy and paste a formatted citation
Spandidos Publications style
Xiong Y, Wang J, Zhu H, Liu L and Jiang Y: Chronic oxymatrine treatment induces resistance and epithelial‑mesenchymal transition through targeting the long non-coding RNA MALAT1 in colorectal cancer cells Retraction in /10.3892/or.2024.8732. Oncol Rep 39: 967-976, 2018.
APA
Xiong, Y., Wang, J., Zhu, H., Liu, L., & Jiang, Y. (2018). Chronic oxymatrine treatment induces resistance and epithelial‑mesenchymal transition through targeting the long non-coding RNA MALAT1 in colorectal cancer cells Retraction in /10.3892/or.2024.8732. Oncology Reports, 39, 967-976. https://doi.org/10.3892/or.2018.6204
MLA
Xiong, Y., Wang, J., Zhu, H., Liu, L., Jiang, Y."Chronic oxymatrine treatment induces resistance and epithelial‑mesenchymal transition through targeting the long non-coding RNA MALAT1 in colorectal cancer cells Retraction in /10.3892/or.2024.8732". Oncology Reports 39.3 (2018): 967-976.
Chicago
Xiong, Y., Wang, J., Zhu, H., Liu, L., Jiang, Y."Chronic oxymatrine treatment induces resistance and epithelial‑mesenchymal transition through targeting the long non-coding RNA MALAT1 in colorectal cancer cells Retraction in /10.3892/or.2024.8732". Oncology Reports 39, no. 3 (2018): 967-976. https://doi.org/10.3892/or.2018.6204
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong Y, Wang J, Zhu H, Liu L and Jiang Y: Chronic oxymatrine treatment induces resistance and epithelial‑mesenchymal transition through targeting the long non-coding RNA MALAT1 in colorectal cancer cells Retraction in /10.3892/or.2024.8732. Oncol Rep 39: 967-976, 2018.
APA
Xiong, Y., Wang, J., Zhu, H., Liu, L., & Jiang, Y. (2018). Chronic oxymatrine treatment induces resistance and epithelial‑mesenchymal transition through targeting the long non-coding RNA MALAT1 in colorectal cancer cells Retraction in /10.3892/or.2024.8732. Oncology Reports, 39, 967-976. https://doi.org/10.3892/or.2018.6204
MLA
Xiong, Y., Wang, J., Zhu, H., Liu, L., Jiang, Y."Chronic oxymatrine treatment induces resistance and epithelial‑mesenchymal transition through targeting the long non-coding RNA MALAT1 in colorectal cancer cells Retraction in /10.3892/or.2024.8732". Oncology Reports 39.3 (2018): 967-976.
Chicago
Xiong, Y., Wang, J., Zhu, H., Liu, L., Jiang, Y."Chronic oxymatrine treatment induces resistance and epithelial‑mesenchymal transition through targeting the long non-coding RNA MALAT1 in colorectal cancer cells Retraction in /10.3892/or.2024.8732". Oncology Reports 39, no. 3 (2018): 967-976. https://doi.org/10.3892/or.2018.6204
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