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DNA methylation enzyme inhibitor RG108 suppresses the radioresistance of esophageal cancer

  • Authors:
    • Yao Ou
    • Quan Zhang
    • Yiting Tang
    • Zhonghua Lu
    • Xujing Lu
    • Xifa Zhou
    • Changmin Liu
  • View Affiliations / Copyright

    Affiliations: Department of Radiotherapy, Changzhou Tumor Hospital, Soochow University, Changzhou, Jiangsu 213001, P.R. China, Department of Radiotherapy, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China, Department of Oncology, The Affiliated Hospital of Binzhou Medical College, Binzhou, Shandong 256603, P.R. China
    Copyright: © Ou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 993-1002
    |
    Published online on: January 11, 2018
       https://doi.org/10.3892/or.2018.6210
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Abstract

Esophageal cancer (EC) is the eighth most common highly aggressive cancer worldwide. The purpose of this study was to investigate the effect of the DNA methyltransferase inhibitor RG108 on the radiosensitivity of EC cells. MTT and clonogenic assays were performed to assess the effect of RG108 on the proliferation and radiosensitivity of Eca‑109 and TE‑1 human EC cells. The cell cycle progression and alterations in apoptosis were analyzed by flow cytometry. For the in vivo analysis, the Eca‑109 cells were inoculated into nude mice to establish tumors. Tissues from xenografts were obtained to detect changes to microvessels and tumor growth by immunohistochemistry (IHC). RNA-seq was used to identify differentially expressed genes. We found that RG108 increased the radiosensitivity of EC cells. Apoptosis and G2/M-phase arrest were induced by X-ray irradiation and were significantly enhanced by RG108. In addition, growth of tumor xenografts from the Eca‑109 cells was significantly inhibited by irradiation in combination with RG108. The RNA-seq analysis revealed that, compared with radiation alone, X-ray irradiation in combination with RG108 altered the expression of 121 genes in multiple pathways, including the TGF-β signaling pathway and the Epstein-Barr virus infection pathway. In conclusion, RG108 induced radiosensitivity in EC cells both in vitro and in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Ou Y, Zhang Q, Tang Y, Lu Z, Lu X, Zhou X and Liu C: DNA methylation enzyme inhibitor RG108 suppresses the radioresistance of esophageal cancer. Oncol Rep 39: 993-1002, 2018.
APA
Ou, Y., Zhang, Q., Tang, Y., Lu, Z., Lu, X., Zhou, X., & Liu, C. (2018). DNA methylation enzyme inhibitor RG108 suppresses the radioresistance of esophageal cancer. Oncology Reports, 39, 993-1002. https://doi.org/10.3892/or.2018.6210
MLA
Ou, Y., Zhang, Q., Tang, Y., Lu, Z., Lu, X., Zhou, X., Liu, C."DNA methylation enzyme inhibitor RG108 suppresses the radioresistance of esophageal cancer". Oncology Reports 39.3 (2018): 993-1002.
Chicago
Ou, Y., Zhang, Q., Tang, Y., Lu, Z., Lu, X., Zhou, X., Liu, C."DNA methylation enzyme inhibitor RG108 suppresses the radioresistance of esophageal cancer". Oncology Reports 39, no. 3 (2018): 993-1002. https://doi.org/10.3892/or.2018.6210
Copy and paste a formatted citation
x
Spandidos Publications style
Ou Y, Zhang Q, Tang Y, Lu Z, Lu X, Zhou X and Liu C: DNA methylation enzyme inhibitor RG108 suppresses the radioresistance of esophageal cancer. Oncol Rep 39: 993-1002, 2018.
APA
Ou, Y., Zhang, Q., Tang, Y., Lu, Z., Lu, X., Zhou, X., & Liu, C. (2018). DNA methylation enzyme inhibitor RG108 suppresses the radioresistance of esophageal cancer. Oncology Reports, 39, 993-1002. https://doi.org/10.3892/or.2018.6210
MLA
Ou, Y., Zhang, Q., Tang, Y., Lu, Z., Lu, X., Zhou, X., Liu, C."DNA methylation enzyme inhibitor RG108 suppresses the radioresistance of esophageal cancer". Oncology Reports 39.3 (2018): 993-1002.
Chicago
Ou, Y., Zhang, Q., Tang, Y., Lu, Z., Lu, X., Zhou, X., Liu, C."DNA methylation enzyme inhibitor RG108 suppresses the radioresistance of esophageal cancer". Oncology Reports 39, no. 3 (2018): 993-1002. https://doi.org/10.3892/or.2018.6210
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