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Article

Targeting bladder cancer using activated T cells armed with bispecific antibodies

  • Authors:
    • Juan Ma
    • Jing Ge
    • Xin Xue
    • Weigang Xiu
    • Pan Ma
    • Ximing Sun
    • Man Zhang
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, P.R. China, Department of Immunology, China Basic Medical Theory of Chinese Medicine, Academy of Chinese Medical Sciences, Beijing 100700, P.R. China, Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P.R. China
  • Pages: 1245-1252
    |
    Published online on: January 11, 2018
       https://doi.org/10.3892/or.2018.6211
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Abstract

In the present study, we aimed to investigate whether EGFR or HER2 may serve as a target for T cell-mediated immunotherapy against human bladder cancer. Expression of EGFR and HER2 was detected on the surface of bladder cancer cells, including Pumc-91 and T24 cells, and their chemotherapeutic drug-resistant counterparts. Activated T cells (ATCs) were generated from healthy PBMCs that were stimulated by the combination of anti-CD3 monoclonal antibody and anti‑CD28 monoclonal antibody in the presence of interleukin-2 for 14 days. The ATCs were then armed with chemically hetero-conjugated anti-CD3xanti-EGFR (EGFRBi-Ab) or anti-CD3xanti-HER2 (HER2Bi-Ab). The specific cytolytic activity of ATCs armed with EGFRBi-Ab or HER2Bi-Ab against human bladder cancer cells was evaluated by lactate dehydrogenase activity assays in vitro. In contrast to unarmed ATCs, EGFRBi-Ab-armed ATCs and HER2Bi-Ab-armed ATCs showed increased cytotoxic activity against bladder cancer cells. Moreover, Bi-Ab-armed ATCs expressed higher levels of activating marker CD69 and secreted more IFN-γ, TNF-α and IL-2 than did unarmed ATCs. EGFRBi-Ab- or HER2Bi-Ab-armed ATCs may provide a promising immunotherapy for bladder cancer.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Pang C, Guan Y, Li H, Chen W and Zhu G: Urologic cancer in China. Jpn J Clin Oncol. 46:497–501. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Von der Maase H, Sengelov L, Roberts JT, Ricci S, Dogliotti L, Oliver T, Moore MJ, Zimmermann A and Arning M: Long-term survival results of a randomized trial comparing gemcitabine plus cisplatin, with methotrexate, vinblastine, doxorubicin, plus cisplatin in patients with bladder cancer. J Clin Oncol. 23:4602–4608. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Krishna R and Mayer LD: Multidrug resistance (MDR) in cancer. Mechanisms, reversal using modulators of MDR and the role of MDR modulators in influencing the pharmacokinetics of anticancer drugs. Eur J Pharm Sci. 11:265–283. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Carneiro BA, Meeks JJ, Kuzel TM, Scaranti M, Abdulkadir SA and Giles FJ: Emerging therapeutic targets in bladder cancer. Cancer Treat Rev. 41:170–178. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Appert-Collin A, Hubert P, Crémel G and Bennasroune A: Role of ErbB receptor in cancer cell migration and invasion. Front Pharmacolo. 6:2832015.

7 

Smolensky D, Rathore K and Cekanova M: Molecular targets in urothelialcancer: Detection, treatment, and animal models ofbladder cancer. Drug Des Devel Ther. 10:3305–3322. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Koletsas N, Koletsa T, Choidas S, Anaqnostopoulos K, Touloupidis S, Zaramboukas T, Raptou G, Papadopoulos N and Lambropoulou M: Immunohistochemical investigation of HER/AKT/mTOR pathway and cellular adhesion molecules in urothelial carcinomas. Patholog Res Int. 2017:67941502017.PubMed/NCBI

9 

Zhao J, Xu W, Zhang Z, Song R, Zeng S, Sun Y and Xu C: Prognostic role of HER2 expression in bladder cancer: A systematic review and meta-analysis. Int Urol Nephrol. 47:87–94. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Ross JS, Slodkowska EA, Symmans WF, Pusztai L, Ravdin PM and Hortobagyi GN: The HER-2 receptor and breast cancer: Ten years of targeted anti-HER-2 therapy and personalized medicine. Oncologist. 14:320–368. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Lo Nigro C, Ricci V, Vivenza D, Granetto C, Fabozzi T, Miraqlio E and Merlano MC: Prognostic and predictive biomarkers in metastatic colorectal canceranti-EGFR therapy. World J Gastroenterol. 22:6944–6954. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Hussain M, Daignault S, Agarwal N, Grivas PD, Siefker-Radtke AO, Puzanov I, MacVicar GR, Levine EG, Srinivas S, Twardowski P, et al: A randomized phase 2 trial of gemcitabine/cisplatin with or without cetuximab in patients with advanced urothelial carcinoma. Cancer. 120:2684–2693. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Philips GK, Halabi S, Sanford BL, Bajorin D and Small EJ; Cancer and Leukaemia Group B, : A phase II trial of cisplatin, fixed dose-rate gemcitabine and gefitinib for advanced urothelial tract carcinoma: Results of the Cancer and Leukaemia Group B 90102. BJU Int. 101:20–25. 2008.PubMed/NCBI

14 

Oudard S, Culine S, Vano Y, Goldwasser F, Théodore C, Nguyen T, Voog E, Banu E, Vieillefond A, Priou F, et al: Multicentre randomized phase II trial of gemcitabine + platinum, with or without trastuzumab, in advanced or metastatic urothelial carcinoma overexpressing Her2. Eur J Cancer. 51:45–54. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Powles T, Huddart RA, Elliott T, Sarker SJ, Ackerman C, Jones R, Hussain S, Crabb S, Jagdev S, Chester J, et al: Phase III, doubled-blind, randomized trial that compared maintenance lapatinib versus placebo after first-line chemotherapy in patients with human epidermal growth factor receptor 1/2-positive metastatic bladder cancer. J Clin Oncol. 35:48–55. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Wu Y, Enting D, Rudman S and Chowdhury S: Immunotherapy for urothelial cancer: From BCG to checkpoint inhibitors and beyond. Expert Rev Anticancer Ther. 15:509–523. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Rosenberg JE, Hoffman-Censits J, Powles T, van der Heijden MS, Balar AV, Necchi A, Dawson N, O'Donnell PH, Balmanoukian A, Loriot Y, et al: Atezolizumab in patients with locally advanced and metastatic urothelial carcinoma who have progressed following treatment with platinum-based chemotherapy: A single-arm, multicentre, phase 2 trial. Lancet. 387:1909–1920. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Sharma P, Callahan MK, Bono P, Kim J, Spiliopoulou P, Calvo E, Pillai RN, Ott PA, de Braud F, Morse M, et al: Nivolumab monotherapy in recurrent metastatic urothelial carcinoma (CheckMate 032): A multicentre, open-label, two-stage, multi-arm, phase 1/2 trial. Lancet Oncol. 17:1590–1598. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Bellmunt J, De Wit R, Vaughn DJ, Fradet Y, Lee JL, Fong L, Vogelzang NJ, Climent MA, Petrylak DP, Choueiri TK, et al: Pembrolizumab as second-line therapy for advanced urothelial carcioma. N Eng J Med. 376:1015–1026. 2017. View Article : Google Scholar

20 

Lum LG, Thakur A, Al-Kadhimi Z, Colvin GA, Cummings FJ, Legare RD, Dizon DS, Kouttab N, Maizei A, Colaiace W, et al: Targeted T-cell therapy in stage IV breast cancer: A phase I clinical trial. Clin Cancer Res. 21:2305–2314. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Vaishampayan U, Thakur A, Rathore R, Kouttab N and Lum LG: Phase I study of anti-CD3 × anti-Her2 bispecific antibody in metastatic castrate resistant prostate cancer patients. Prostate Cancer. 2015:2851932015. View Article : Google Scholar : PubMed/NCBI

22 

Zhao M, Zhang M and Ma P: Establishment of cisplatin-multi drug resistance cell line of human bladder cancer T24 and its biological characteristics. Immun Clin Med. 22:338–341. 2015.(In Chinese).

23 

Wang S, Lei T and Zhang M: The reversal effect and its mechanisms of tetramethylpyrazine on multidrug resistance in human bladder cancer. PLoS One. 11:e01577592016. View Article : Google Scholar : PubMed/NCBI

24 

Zhang M, Jin S and Zhang M: The establishment and biological characteristics evaluation of human multidrug-resistant bladder cancer Pumc-91/ADM cell line. J Med Res. 38:70–72. 2009.(In Chinese).

25 

Wang S, Meng Q, Xie Qi and Zhang M: Effect and mechanism of resveratrol on drug resistance in human bladder cancer cells. Mol Med Rep. 15:1179–1187. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Xiu W, Ma J, Lei T, Zhang M and Zhou S: Immunosuppressive effect of bladder cancer on function of dendritic cells involving of Jak2/STAT3 pathway. Oncotarget. 7:63204–63214. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Ma P, He Q, Li W, Li X, Han H, Jin M, Liu C, Tao H, Ma J and Gao B: Anti-CD3 × EGFR bispecific antibody redirects cytokine-induced killer cells to glioblastoma in vitro and in vivo. Oncol Rep. 34:2567–2575. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Han H, Ma J, Zhang K, Li W, Liu C, Zhang Y, Zhang G, Ma P, Wang L, Zhang G, et al: Bispecific anti-CD3 × anti-HER2 antibody mediates T cell cytolytic activity to HER2-positive colorectal cancer in vitro and in vivo. Int J Oncol. 45:2446–2454. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Ma J, Han H, Liu D, Li W, Feng H, Xue X, Wu X, Niu G, Zhang G, Zhao Y, et al: HER2 as a promising target for cytotoxicity T cells in human melanoma therapy. PLoS One. 8:e732612013. View Article : Google Scholar : PubMed/NCBI

30 

Ma J, Ma P, Zhao C, Xue X, Han H, Liu C, Tao H, Xiu W, Cai J and Zhang M: B7-H3 as a promising target for cytotoxicity T cell in human cancer therapy. Oncotarget. 7:29480–29491. 2016.PubMed/NCBI

31 

Lum LG and Thakur A: Targeting T cells with bispecific antibodies for cancer therapy. BioDrugs. 25:365–379. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Bhutani D and Lum LG: Activated T cells armed with bispecific antibodies killtumor targets. Curr Opin Hematol. 22:476–483. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Friedman M, Lindström S, Ekerljumg L, Andersson-Svahn H, Carlsson J, Brismar H, Gedda L, Frejd FY and Ståhl S: Engineering and characterization of a bispecific HER2 × EGFR-binding affibody molecule. Biotechnol Appl. Biochem. 54:121–131. 2009.

34 

Sathaliyawala T, Kubota M, Yudanin N, Turner D, Camp P, Thome JJ, Bickham KL, Lerner H, Goldstein M, Sykes M, et al: Distribution and compartmentalization of human circulating and tissue-resident memory T cell subsets. Immunity. 38:187–197. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ma J, Ge J, Xue X, Xiu W, Ma P, Sun X and Zhang M: Targeting bladder cancer using activated T cells armed with bispecific antibodies. Oncol Rep 39: 1245-1252, 2018.
APA
Ma, J., Ge, J., Xue, X., Xiu, W., Ma, P., Sun, X., & Zhang, M. (2018). Targeting bladder cancer using activated T cells armed with bispecific antibodies. Oncology Reports, 39, 1245-1252. https://doi.org/10.3892/or.2018.6211
MLA
Ma, J., Ge, J., Xue, X., Xiu, W., Ma, P., Sun, X., Zhang, M."Targeting bladder cancer using activated T cells armed with bispecific antibodies". Oncology Reports 39.3 (2018): 1245-1252.
Chicago
Ma, J., Ge, J., Xue, X., Xiu, W., Ma, P., Sun, X., Zhang, M."Targeting bladder cancer using activated T cells armed with bispecific antibodies". Oncology Reports 39, no. 3 (2018): 1245-1252. https://doi.org/10.3892/or.2018.6211
Copy and paste a formatted citation
x
Spandidos Publications style
Ma J, Ge J, Xue X, Xiu W, Ma P, Sun X and Zhang M: Targeting bladder cancer using activated T cells armed with bispecific antibodies. Oncol Rep 39: 1245-1252, 2018.
APA
Ma, J., Ge, J., Xue, X., Xiu, W., Ma, P., Sun, X., & Zhang, M. (2018). Targeting bladder cancer using activated T cells armed with bispecific antibodies. Oncology Reports, 39, 1245-1252. https://doi.org/10.3892/or.2018.6211
MLA
Ma, J., Ge, J., Xue, X., Xiu, W., Ma, P., Sun, X., Zhang, M."Targeting bladder cancer using activated T cells armed with bispecific antibodies". Oncology Reports 39.3 (2018): 1245-1252.
Chicago
Ma, J., Ge, J., Xue, X., Xiu, W., Ma, P., Sun, X., Zhang, M."Targeting bladder cancer using activated T cells armed with bispecific antibodies". Oncology Reports 39, no. 3 (2018): 1245-1252. https://doi.org/10.3892/or.2018.6211
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