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Article Open Access

Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier

  • Authors:
    • Mikhail K. Ivanov
    • Sergei E. Titov
    • Sergei A. Glushkov
    • Victoria V. Dzyubenko
    • Anastasia V. Malek
    • Polina A. Arkhangelskaya
    • Roman B. Samsonov
    • Andrey A. Mikhetko
    • Elena V. Bakhidze
    • Igor V. Berlev
    • Nikolay N. Kolesnikov
  • View Affiliations / Copyright

    Affiliations: AO Vector-Best, 630117 Novosibirsk, Russia, N.N. Petrov Research Institute of Oncology, 197758 Saint Petersburg, Russia, Institute of Molecular and Cellular Biology, Siberian Branch of The Russian Academy of Sciences, 630090 Novosibirsk, Russia
    Copyright: © Ivanov et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1099-1111
    |
    Published online on: January 12, 2018
       https://doi.org/10.3892/or.2018.6214
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Abstract

Recent studies have shown that changes in the expression levels of certain microRNAs correlate with the degree of severity of cervical lesions. The aim of the present study was to develop a microRNA-based classifier for the detection of high-grade cervical intraepithelial neoplasia (CIN ≥2) in cytological samples from patients with different high-risk human papillomavirus (HR-HPV) viral loads. For this purpose, raw RT-qPCR data for 25 candidate microRNAs, U6 snRNA and human DNA in air-dried PAP smears from 174 women with different cervical cytological diagnoses, 144 of which were HR-HPV-positive [40 negative for intraepithelial lesion or malignancy (NILM), 34 low-grade squamous intraepithelial lesions (L-SIL), 57 high-grade squamous intraepithelial lesions (H-SIL), 43 invasive cancers], were statistically processed. The expression level changes of various individual microRNAs were found to be significantly correlated with the cytological diagnosis but the statistical significance of this correlation was critically dependent on the normalization strategy. We developed a linear classifier based on the paired ratios of 8 microRNA concentrations and cellular DNA content. The classifier determines the dimensionless coefficient (DF value), which increases with the severity of cervical lesion. The high- and low-grade CINs were better distinguished by the microRNA classifier than by the measurement of individual microRNA levels with the use of traditional normalization methods. The diagnostic sensitivity of detecting high-grade lesions (CIN ≥2) with the developed microRNA classifier was 83.4%, diagnostic specificity 81.2%, ROC AUC=0.913. The analysis can be performed with the same nucleic acid preparation as used for HPV testing. No statistically significant correlation of the DF value and HR-HPV DNA load was found. The DF value and the HR HPV presence and viral DNA load may be regarded as independent criteria that can complement each other in molecular screening for high-grade cervical intraepithelial neoplasia. Although it has several limitations, the present study showed that the small-scale analysis of microRNA signatures performed by simple PCR-based methods may be useful for improving the diagnostic/prognostic value of cervical screening.
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Copy and paste a formatted citation
Spandidos Publications style
Ivanov MK, Titov SE, Glushkov SA, Dzyubenko VV, Malek AV, Arkhangelskaya PA, Samsonov RB, Mikhetko AA, Bakhidze EV, Berlev IV, Berlev IV, et al: Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier. Oncol Rep 39: 1099-1111, 2018.
APA
Ivanov, M.K., Titov, S.E., Glushkov, S.A., Dzyubenko, V.V., Malek, A.V., Arkhangelskaya, P.A. ... Kolesnikov, N.N. (2018). Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier. Oncology Reports, 39, 1099-1111. https://doi.org/10.3892/or.2018.6214
MLA
Ivanov, M. K., Titov, S. E., Glushkov, S. A., Dzyubenko, V. V., Malek, A. V., Arkhangelskaya, P. A., Samsonov, R. B., Mikhetko, A. A., Bakhidze, E. V., Berlev, I. V., Kolesnikov, N. N."Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier". Oncology Reports 39.3 (2018): 1099-1111.
Chicago
Ivanov, M. K., Titov, S. E., Glushkov, S. A., Dzyubenko, V. V., Malek, A. V., Arkhangelskaya, P. A., Samsonov, R. B., Mikhetko, A. A., Bakhidze, E. V., Berlev, I. V., Kolesnikov, N. N."Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier". Oncology Reports 39, no. 3 (2018): 1099-1111. https://doi.org/10.3892/or.2018.6214
Copy and paste a formatted citation
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Spandidos Publications style
Ivanov MK, Titov SE, Glushkov SA, Dzyubenko VV, Malek AV, Arkhangelskaya PA, Samsonov RB, Mikhetko AA, Bakhidze EV, Berlev IV, Berlev IV, et al: Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier. Oncol Rep 39: 1099-1111, 2018.
APA
Ivanov, M.K., Titov, S.E., Glushkov, S.A., Dzyubenko, V.V., Malek, A.V., Arkhangelskaya, P.A. ... Kolesnikov, N.N. (2018). Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier. Oncology Reports, 39, 1099-1111. https://doi.org/10.3892/or.2018.6214
MLA
Ivanov, M. K., Titov, S. E., Glushkov, S. A., Dzyubenko, V. V., Malek, A. V., Arkhangelskaya, P. A., Samsonov, R. B., Mikhetko, A. A., Bakhidze, E. V., Berlev, I. V., Kolesnikov, N. N."Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier". Oncology Reports 39.3 (2018): 1099-1111.
Chicago
Ivanov, M. K., Titov, S. E., Glushkov, S. A., Dzyubenko, V. V., Malek, A. V., Arkhangelskaya, P. A., Samsonov, R. B., Mikhetko, A. A., Bakhidze, E. V., Berlev, I. V., Kolesnikov, N. N."Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier". Oncology Reports 39, no. 3 (2018): 1099-1111. https://doi.org/10.3892/or.2018.6214
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