A 60 kDa prolactin variant secreted by cervical cancer cells modulates apoptosis and cytokine production

  • Authors:
    • Adrián Ramírez De Arellano
    • Annie Riera Leal
    • Edgar I. Lopez-Pulido
    • Luis Renee González-Lucano
    • José Macías Barragan
    • Susana Del Toro Arreola
    • Mariel García-Chagollan
    • Claudia A. Palafox-Sánchez
    • José F. Muñoz-Valle
    • Ana Laura Pereira-Suárez
  • View Affiliations

  • Published online on: January 19, 2018     https://doi.org/10.3892/or.2018.6222
  • Pages: 1253-1260
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Abstract

Prolactin (PRL) is associated with different types of cancer, such as cervical cancer. Recombinant PRL has antiapoptotic effect on cervical cancer cells, and it can also induce cytokine production on macrophages. A 60 kDa variant of PRL is produced by cervical cancer cells. The aim of the present study was to evaluate this variant's bioactivity, to test its effect on cervical cancer cell apoptosis, and to assess its ability to induce cytokine production on THP-1 macrophages. First, 60 kDa PRL was isolated and used to stimulate Nb2 cells. Later, apoptosis was measured after exposure to 60 kDa PRL. Finally, cytokines were measured on THP-1 stimulated supernatants. Our results show that 60 kDa PRL increased Nb2 cell proliferation. Apoptosis was decreased after stimuli with 60 kDa PRL in cervical cancer cells. IL-1β and TNF-α are produced by THP-1 macrophages after stimuli. These results suggest that 60 kDa PRL produced by cervical cancer cells is able to reduce apoptosis in HeLa, SiHa and C-33A cells and induce IL-1β and TNF-α production by THP-1 macrophages.
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March-2018
Volume 39 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ramírez De Arellano A, Riera Leal A, Lopez-Pulido EI, González-Lucano L, Macías Barragan J, Del Toro Arreola S, García-Chagollan M, Palafox-Sánchez CA, Muñoz-Valle JF, Pereira-Suárez AL, Pereira-Suárez AL, et al: A 60 kDa prolactin variant secreted by cervical cancer cells modulates apoptosis and cytokine production. Oncol Rep 39: 1253-1260, 2018
APA
Ramírez De Arellano, A., Riera Leal, A., Lopez-Pulido, E.I., González-Lucano, L., Macías Barragan, J., Del Toro Arreola, S. ... Pereira-Suárez, A.L. (2018). A 60 kDa prolactin variant secreted by cervical cancer cells modulates apoptosis and cytokine production. Oncology Reports, 39, 1253-1260. https://doi.org/10.3892/or.2018.6222
MLA
Ramírez De Arellano, A., Riera Leal, A., Lopez-Pulido, E. I., González-Lucano, L., Macías Barragan, J., Del Toro Arreola, S., García-Chagollan, M., Palafox-Sánchez, C. A., Muñoz-Valle, J. F., Pereira-Suárez, A. L."A 60 kDa prolactin variant secreted by cervical cancer cells modulates apoptosis and cytokine production". Oncology Reports 39.3 (2018): 1253-1260.
Chicago
Ramírez De Arellano, A., Riera Leal, A., Lopez-Pulido, E. I., González-Lucano, L., Macías Barragan, J., Del Toro Arreola, S., García-Chagollan, M., Palafox-Sánchez, C. A., Muñoz-Valle, J. F., Pereira-Suárez, A. L."A 60 kDa prolactin variant secreted by cervical cancer cells modulates apoptosis and cytokine production". Oncology Reports 39, no. 3 (2018): 1253-1260. https://doi.org/10.3892/or.2018.6222