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Article Open Access

Effect of sinomenine hydrochloride on radiosensitivity of esophageal squamous cell carcinoma cells

  • Authors:
    • Shenbo Fu
    • Long Jin
    • Tuotuo Gong
    • Shupei Pan
    • Shuyu Zheng
    • Xuanwei Zhang
    • Tian Yang
    • Yuchen Sun
    • Ya Wang
    • Jia Guo
    • Beina Hui
    • Xiaozhi Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Radiation Oncology, Shaanxi Province People's Hospital, Xi'an, Shaanxi 710068, P.R. China
    Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1601-1608
    |
    Published online on: January 22, 2018
       https://doi.org/10.3892/or.2018.6228
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Abstract

Radiation therapy is one of the most important treatments for unresectable and locally advanced esophageal squamous cell carcinoma (ESCC), however, the response to radiotherapy is sometimes limited by the development of radioresistance. Sinomenine hydrochloride (SH) has anticancer activity, but its effect on the radiosensitivity of ESCC is unclear. We determined the effect of SH on the radiosensitivity of ESCC cells and elucidated its potential radiosensitization mechanisms in vitro and in vivo. ESCC cells were subjected to SH and radiation, both separately and in combination. Untreated cells served as controls. The CCK‑8 assay was used to evaluate cell proliferation, and the clonogenic assay to estimate radiosensitization. Flow cytometry was used to investigate cell cycle phases and cell apoptosis. Bcl‑2, Bax, cyclin B1, CDK1, Ku86, Ku70, and Rad51 expression was evaluated using western blotting. In vivo, tumor xenografts were created using BALB/c nude mice. Tumor‑growth inhibition was recorded, and Ki‑67 and Bax expression in the tumor tissues was assessed using immunohistochemistry. SH inhibited ESCC cell growth and markedly increased their radiosensitivity by inducing G2/M phase arrest. SH combined with radiation therapy significantly increased ESCC cell apoptosis. The molecular mechanism by which SH enhanced radiosensitivity of ESCC cells was related to Bcl‑2, cyclin B1, CDK1, Ku86, Ku70, and Rad51 downregulation and Bax protein expression upregulation. SH combined with radiation considerably delayed the growth of tumor xenografts in vivo. Immunohistochemical analysis showed that in the SH combined with radiation group, the expression of Bax was significantly higher while that of Ki‑67 was lower than the expressions in the control groups. Taken together, our findings showed that SH could improve the sensitivity of radiation in ESCC cells by inducing G2/M phase arrest, promoting radiation‑induced apoptosis and inhibiting DSB‑repair pathways. SH appears to be a prospective radiosensitizer for improving the efficacy of radiotherapy for ESCC.
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Copy and paste a formatted citation
Spandidos Publications style
Fu S, Jin L, Gong T, Pan S, Zheng S, Zhang X, Yang T, Sun Y, Wang Y, Guo J, Guo J, et al: Effect of sinomenine hydrochloride on radiosensitivity of esophageal squamous cell carcinoma cells. Oncol Rep 39: 1601-1608, 2018.
APA
Fu, S., Jin, L., Gong, T., Pan, S., Zheng, S., Zhang, X. ... Zhang, X. (2018). Effect of sinomenine hydrochloride on radiosensitivity of esophageal squamous cell carcinoma cells. Oncology Reports, 39, 1601-1608. https://doi.org/10.3892/or.2018.6228
MLA
Fu, S., Jin, L., Gong, T., Pan, S., Zheng, S., Zhang, X., Yang, T., Sun, Y., Wang, Y., Guo, J., Hui, B., Zhang, X."Effect of sinomenine hydrochloride on radiosensitivity of esophageal squamous cell carcinoma cells". Oncology Reports 39.4 (2018): 1601-1608.
Chicago
Fu, S., Jin, L., Gong, T., Pan, S., Zheng, S., Zhang, X., Yang, T., Sun, Y., Wang, Y., Guo, J., Hui, B., Zhang, X."Effect of sinomenine hydrochloride on radiosensitivity of esophageal squamous cell carcinoma cells". Oncology Reports 39, no. 4 (2018): 1601-1608. https://doi.org/10.3892/or.2018.6228
Copy and paste a formatted citation
x
Spandidos Publications style
Fu S, Jin L, Gong T, Pan S, Zheng S, Zhang X, Yang T, Sun Y, Wang Y, Guo J, Guo J, et al: Effect of sinomenine hydrochloride on radiosensitivity of esophageal squamous cell carcinoma cells. Oncol Rep 39: 1601-1608, 2018.
APA
Fu, S., Jin, L., Gong, T., Pan, S., Zheng, S., Zhang, X. ... Zhang, X. (2018). Effect of sinomenine hydrochloride on radiosensitivity of esophageal squamous cell carcinoma cells. Oncology Reports, 39, 1601-1608. https://doi.org/10.3892/or.2018.6228
MLA
Fu, S., Jin, L., Gong, T., Pan, S., Zheng, S., Zhang, X., Yang, T., Sun, Y., Wang, Y., Guo, J., Hui, B., Zhang, X."Effect of sinomenine hydrochloride on radiosensitivity of esophageal squamous cell carcinoma cells". Oncology Reports 39.4 (2018): 1601-1608.
Chicago
Fu, S., Jin, L., Gong, T., Pan, S., Zheng, S., Zhang, X., Yang, T., Sun, Y., Wang, Y., Guo, J., Hui, B., Zhang, X."Effect of sinomenine hydrochloride on radiosensitivity of esophageal squamous cell carcinoma cells". Oncology Reports 39, no. 4 (2018): 1601-1608. https://doi.org/10.3892/or.2018.6228
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