Knockdown of angiopoietin-like 4 inhibits the development of human gastric cancer

Chen,Jin-Wu; Luo,Ying-Jia; Yang,Zheng-Fei; Wen,Li-Qiang; Huang,Lin

doi:10.3892/or.2018.6253

Knockdown of angiopoietin-like 4 inhibits the development of human gastric cancer

Authors:
- Jin-Wu Chen
- Ying-Jia Luo
- Zheng-Fei Yang
- Li-Qiang Wen
- Lin Huang
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Affiliations: Medical Examination Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, P.R. China, Department of Teaching and Research of Diagnostics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, P.R. China, Department of Emergency, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, P.R. China, Center for Spine and Pelvic Tumors, Orthopedic Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, P.R. China
Published online on: February 7, 2018 https://doi.org/10.3892/or.2018.6253
Pages: 1739-1746

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Abstract

Human gastric cancer (GC) is the second most common cause of cancer-related deaths worldwide and is one of the most common metastatic cancers. Tumor proliferation, apoptosis, metastasis and invasion are important predictors of the invasiveness of GC and are key factors in cancer-induced death. Angiopoietin-like 4 (ANGPTL4) is a secreted protein that belongs to the angiopoietin (ANGPTL) family and is involved in the regulation of cancer metastasis. However, whether ANGPTL4 plays a role in the progression of GC remain unclear. In the present study, immunoreactivity of ANGPTL4 demonstrated that ANGPTL4 expression was upregulated in GC tissues with the development of GC. The siRNA targeting ANGPTL4 effectively knocked down ANGPTL4 in the SNU‑1 and BGC823 cell lines at the mRNA and protein levels. Following ANGPTL4 downregulation, the proliferation and invasion abilities of GC cell lines were suppressed as determined by MTT and Transwell assays, and cell apoptosis level and sensitivity to cisplatin were increased as determined by flow cytometry and MTT assay. In conclusion, these findings suggest that ANGPTL4 may be a new potential therapeutic target for GC.

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