Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro

  • Authors:
    • Tian Liao
    • Yuan-Jin Wang
    • Jia-Qian Hu
    • Yu Wang
    • Li-Tao Han
    • Ben Ma
    • Rong-Liang Shi
    • Ning Qu
    • Wen-Jun Wei
    • Qing Guan
    • Jun Xiang
    • Jia-Ying Chen
    • Guo-Hua Sun
    • Duan-Shu Li
    • Xiang-Ming Mu
    • Qing-Hai Ji
  • View Affiliations

  • Published online on: March 5, 2018     https://doi.org/10.3892/or.2018.6295
  • Pages: 2185-2192
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Abstract

KMT5A (known as PR-Set7/9, SETD8 and SET8), a member of the SET domain containing methyltransferase family specifically targeting H4K20 for methylation, has been implicated in multiple biological processes. In the present study, we identified that KMT5A was elevated in 50 pairs of papillary thyroid cancer tissue samples and in cell lines K1 and TPC-1 by qRT-PCR and western blotting, as well as by immunohistochemical staining. CCK-8 assay and flow cytometric analysis revealed that inhibition of KMT5A attenuated proliferation and induced apoptosis. Transwell assays revealed that cell migration and invasion were suppressed in KMT5A-knockdown cells. Moreover, the inhibition of KMT5A arrested the cell cycle in the G1/S phase of papillary thyroid cancer cells. The TCGA data revealed that elevated KMT5A expression was significantly correlated with extrathyroidal extension, lymph node metastasis and advanced pathological stage of papillary thyroid cancer. Furthermore, we observed that inhibition of KMT5A suppressed the expression of SREBP1, SCD, FASN and ACC, key molecules involved in lipid metabolism and decreased the level of malondialdehyde in papillary thyroid cancer cells. In conclusion, KMT5A may be a novel oncogenic factor, specifically a regulator for lipid metabolism in papillary thyroid carcinoma.
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May-2018
Volume 39 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Liao T, Wang Y, Hu J, Wang Y, Han L, Ma B, Shi R, Qu N, Wei W, Guan Q, Guan Q, et al: Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro. Oncol Rep 39: 2185-2192, 2018
APA
Liao, T., Wang, Y., Hu, J., Wang, Y., Han, L., Ma, B. ... Ji, Q. (2018). Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro. Oncology Reports, 39, 2185-2192. https://doi.org/10.3892/or.2018.6295
MLA
Liao, T., Wang, Y., Hu, J., Wang, Y., Han, L., Ma, B., Shi, R., Qu, N., Wei, W., Guan, Q., Xiang, J., Chen, J., Sun, G., Li, D., Mu, X., Ji, Q."Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro". Oncology Reports 39.5 (2018): 2185-2192.
Chicago
Liao, T., Wang, Y., Hu, J., Wang, Y., Han, L., Ma, B., Shi, R., Qu, N., Wei, W., Guan, Q., Xiang, J., Chen, J., Sun, G., Li, D., Mu, X., Ji, Q."Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro". Oncology Reports 39, no. 5 (2018): 2185-2192. https://doi.org/10.3892/or.2018.6295