Serum microRNA-139-5p is downregulated in lung cancer patients with lytic bone metastasis

Xu,Song; Yang,Fan; Liu,Renwang; Li,Xiongfei; Fan,Haiyang; Liu,Jinghao; Wei,Sen; Chen,Gang; Chen,Jun; Da,Yurong

doi:10.3892/or.2018.6316

May-2018 Volume 39 Issue 5

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May-2018 Volume 39 Issue 5

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Article

Serum microRNA-139-5p is downregulated in lung cancer patients with lytic bone metastasis

Authors:
- Song Xu
- Fan Yang
- Renwang Liu
- Xiongfei Li
- Haiyang Fan
- Jinghao Liu
- Sen Wei
- Gang Chen
- Jun Chen
- Yurong Da
View Affiliations / Copyright

Affiliations: Department of Lung Cancer Surgery, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Department of Immunology, School of Basic Medical Sciences, Research Center of Basic Medical Science, Tianjin Key Laboratory of Cellular and Molecular Immunology and Key Laboratory of The Educational Ministry of China, Tianjin Medical University, Tianjin 300070, P.R. China
Pages: 2376-2384
|
Published online on: March 15, 2018

https://doi.org/10.3892/or.2018.6316
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Abstract

Bone remodeling can be interrupted by tumor cells which leads to an inappropriate balance of osteoblasts and osteoclasts. As the progenitors of osteoblasts, mesenchymal stem cells (MSCs) have been reported to exhibit an abnormal osteogenic differentiation potential in some cancer‑related bone lesions. However, the evidence is very limited in terms of the biological alterations of MSCs in the bone metastasis of non‑small cell lung cancers (NSCLC). We investigated the expression and function of miR‑139‑5p in MSC osteogenic differentiation in vitro in normal and NSCLC-exposed condition. Then, we compared the serum miR‑139‑5p in stage IV lung adenocarcinoma cancer patients with and without lytic bone metastasis. We found that MSCs exhibited a significant increase in miR‑139‑5p expression after exposure to osteogenic differentiation induction medium. However, Notch1, which was confirmed as a target of miR‑139‑5p by luciferase and western blot assays, showed a marked downregulated expression together with its pathway downstream factors during MSC osteogenesis. miR‑139‑5p positively regulated MSC osteogenic differentiation but this effect was abrogated significantly by Notch1 knockdown. After exposure to conditions of lung cancer cells A549 and L9981, MSCs exhibited significant downregulation of miR‑139‑5p expression and early osteogenic marker ALP activity. Furthermore, we demonstrated that the expression of serum miR‑139‑5p from lung adenocarcinoma patients with lytic bone metastasis was significantly lower compared to that in patients with metastases in other organs. The potential roles of miR‑139‑5p as a biomarker and treatment target in monitoring and controlling bone metastasis in lung cancer patients are worthy of being further explored.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Serum microRNA-139-5p is downregulated in lung cancer patients with lytic bone metastasis

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