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Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer

  • Authors:
    • Yipeng Wang
    • Liping Guo
    • Lin Feng
    • Wen Zhang
    • Ting Xiao
    • Xuebing Di
    • Guoji Chen
    • Kaitai Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P.R. China, State Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P.R. China, State Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P.R. China, Department of Immunology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P.R. China, Department of Breast Surgery, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2147-2159
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    Published online on: March 20, 2018
       https://doi.org/10.3892/or.2018.6325
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Abstract

Circulating tumour cell (CTC) behaviours are distinct from those of bulk tissues. Thus, treatments to eliminate CTCs differ from the regimens followed to reduce the primary tumour and its metastases. Accordingly, comprehensively deciphering the single nucleotide variant (SNV) profiles in CTCs, which partially determine CTC behaviours, is a priority. Using viable CTCs isolated with the oHSV1‑hTERT‑GFP virus coupled with fluorescence‑activated cell sorting (FACS), the whole genome was amplified using the multiple annealing and looping‑based amplification cycle (MALBAC) method. CTC behaviours were evaluated using the SNVs found to be recurrently mutated in different cells (termed CTC‑shared SNVs). Analysis of the sequencing data of 11 CTCs from 8 patients demonstrated that SNVs accumulated sporadically among CTCs and their matched primary tumours (22 co‑occurring mutated genes were identified in the exomes of CTCs and their matched primary tissues and metastases), and 394 SNVs were shared by at least two CTCs. Mutated APC and LRP1B genes co‑occurred in CTC‑shared and bulk‑tissue SNVs. Additionally, the breast‑originating identity of the CTC‑shared SNVs was verified, and they demonstrated the following CTC behaviours: i) intravasation competency; ii) increased migration or motility; iii) enhanced cell‑cell interactions; iv) variation in energy metabolism; v) an activated platelet or coagulation system; and vi) dysfunctional mitosis. These results demonstrated that it is feasible to capture and amplify the genomes of single CTCs using the described pipeline. CTC‑shared SNVs are a potential signature for identifying the origin of the primary tumour in a liquid biopsy. Furthermore, CTCs demonstrated some behaviours that are unique from those of bulk tissues. Therefore, therapies to eradicate these precursors of metastasis may differ from the existing traditional regimens.
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Spandidos Publications style
Wang Y, Guo L, Feng L, Zhang W, Xiao T, Di X, Chen G and Zhang K: Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer. Oncol Rep 39: 2147-2159, 2018.
APA
Wang, Y., Guo, L., Feng, L., Zhang, W., Xiao, T., Di, X. ... Zhang, K. (2018). Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer. Oncology Reports, 39, 2147-2159. https://doi.org/10.3892/or.2018.6325
MLA
Wang, Y., Guo, L., Feng, L., Zhang, W., Xiao, T., Di, X., Chen, G., Zhang, K."Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer". Oncology Reports 39.5 (2018): 2147-2159.
Chicago
Wang, Y., Guo, L., Feng, L., Zhang, W., Xiao, T., Di, X., Chen, G., Zhang, K."Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer". Oncology Reports 39, no. 5 (2018): 2147-2159. https://doi.org/10.3892/or.2018.6325
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Spandidos Publications style
Wang Y, Guo L, Feng L, Zhang W, Xiao T, Di X, Chen G and Zhang K: Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer. Oncol Rep 39: 2147-2159, 2018.
APA
Wang, Y., Guo, L., Feng, L., Zhang, W., Xiao, T., Di, X. ... Zhang, K. (2018). Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer. Oncology Reports, 39, 2147-2159. https://doi.org/10.3892/or.2018.6325
MLA
Wang, Y., Guo, L., Feng, L., Zhang, W., Xiao, T., Di, X., Chen, G., Zhang, K."Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer". Oncology Reports 39.5 (2018): 2147-2159.
Chicago
Wang, Y., Guo, L., Feng, L., Zhang, W., Xiao, T., Di, X., Chen, G., Zhang, K."Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer". Oncology Reports 39, no. 5 (2018): 2147-2159. https://doi.org/10.3892/or.2018.6325
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