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Expression and oncogenic properties of membranous Notch1 in oral leukoplakia and oral squamous cell carcinoma

  • Authors:
    • Xu Ding
    • Yang Zheng
    • Zhao Wang
    • Wei Zhang
    • Yibo Dong
    • Wantao Chen
    • Jiang Li
    • Weiming Chu
    • Yi Zhong
    • Li Mao
    • Xiaomeng Song
    • Yunong Wu
  • View Affiliations / Copyright

    Affiliations: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai 200011, P.R. China, Department of Stomatology, Northern Jiangsu People's Hospital, Jiangsu 225001, P.R. China, Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, University of Maryland, Baltimore, MD 21201, USA
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2584-2594
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    Published online on: March 27, 2018
       https://doi.org/10.3892/or.2018.6335
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Abstract

Notch1 signaling is essential for tissue development and tumor progression. This signaling pathway has also been implicated in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC). However, the role of Notch1 expression in OL and its malignant transformation is unknown. This study aimed to examine the Notch1 expression patterns by immunohistochemistry (IHC) in a cohort of 78 Chinese patients with OL and to analyze the relationship between the patterns and progression of OL to OSCC. Strong Notch1 staining was observed in 10 (13%) of the 78 OL patients, but it was not associated with any of the clinicopathological parameters. However, we observed membranous Notch1 expression in 24 (31%) of the OL samples. Membranous Notch1 expression was significantly associated with the severity of dysplasia (P<0.001) and development of OSCC (P=0.003). By multivariate analysis, membranous Notch1 expression was found to be the only independent factor for OSCC development in the patient population (P=0.019). Among the 24 patients with membranous Notch1 expression, 11 (46%) developed OSCC compared to 8 (15%) of the 54 patients without such expression (P=0.001, determined by log‑rank test). Furthermore, we established a 4‑nitroquinoline‑1‑oxide (4NQO)‑induced murine OSCC model and studied the Notch1 expression patterns in different stages of carcinogenesis. We observed that the extent of expression of membranous Notch1 increased during carcinogenesis. These data indicated a relationship between membranous Notch1 expression and OSCC risk in patients with OL and suggested that membranous Notch1 served as a biomarker for assessing OSCC risk.
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Copy and paste a formatted citation
Spandidos Publications style
Ding X, Zheng Y, Wang Z, Zhang W, Dong Y, Chen W, Li J, Chu W, Zhong Y, Mao L, Mao L, et al: Expression and oncogenic properties of membranous Notch1 in oral leukoplakia and oral squamous cell carcinoma. Oncol Rep 39: 2584-2594, 2018.
APA
Ding, X., Zheng, Y., Wang, Z., Zhang, W., Dong, Y., Chen, W. ... Wu, Y. (2018). Expression and oncogenic properties of membranous Notch1 in oral leukoplakia and oral squamous cell carcinoma. Oncology Reports, 39, 2584-2594. https://doi.org/10.3892/or.2018.6335
MLA
Ding, X., Zheng, Y., Wang, Z., Zhang, W., Dong, Y., Chen, W., Li, J., Chu, W., Zhong, Y., Mao, L., Song, X., Wu, Y."Expression and oncogenic properties of membranous Notch1 in oral leukoplakia and oral squamous cell carcinoma". Oncology Reports 39.6 (2018): 2584-2594.
Chicago
Ding, X., Zheng, Y., Wang, Z., Zhang, W., Dong, Y., Chen, W., Li, J., Chu, W., Zhong, Y., Mao, L., Song, X., Wu, Y."Expression and oncogenic properties of membranous Notch1 in oral leukoplakia and oral squamous cell carcinoma". Oncology Reports 39, no. 6 (2018): 2584-2594. https://doi.org/10.3892/or.2018.6335
Copy and paste a formatted citation
x
Spandidos Publications style
Ding X, Zheng Y, Wang Z, Zhang W, Dong Y, Chen W, Li J, Chu W, Zhong Y, Mao L, Mao L, et al: Expression and oncogenic properties of membranous Notch1 in oral leukoplakia and oral squamous cell carcinoma. Oncol Rep 39: 2584-2594, 2018.
APA
Ding, X., Zheng, Y., Wang, Z., Zhang, W., Dong, Y., Chen, W. ... Wu, Y. (2018). Expression and oncogenic properties of membranous Notch1 in oral leukoplakia and oral squamous cell carcinoma. Oncology Reports, 39, 2584-2594. https://doi.org/10.3892/or.2018.6335
MLA
Ding, X., Zheng, Y., Wang, Z., Zhang, W., Dong, Y., Chen, W., Li, J., Chu, W., Zhong, Y., Mao, L., Song, X., Wu, Y."Expression and oncogenic properties of membranous Notch1 in oral leukoplakia and oral squamous cell carcinoma". Oncology Reports 39.6 (2018): 2584-2594.
Chicago
Ding, X., Zheng, Y., Wang, Z., Zhang, W., Dong, Y., Chen, W., Li, J., Chu, W., Zhong, Y., Mao, L., Song, X., Wu, Y."Expression and oncogenic properties of membranous Notch1 in oral leukoplakia and oral squamous cell carcinoma". Oncology Reports 39, no. 6 (2018): 2584-2594. https://doi.org/10.3892/or.2018.6335
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