Critical role for non‑GAP function of Gαs in RGS1‑mediated promotion of melanoma progression through AKT and ERK phosphorylation

Sun,Meng‑Yan; Wang,Yuchong; Zhu,Ji; Lv,Chuan; Wu,Kai; Wang,Xin‑Wei; Xue,Chun‑Yu

doi:10.3892/or.2018.6341

June-2018 Volume 39 Issue 6

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June-2018 Volume 39 Issue 6

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Article

Critical role for non‑GAP function of Gαs in RGS1‑mediated promotion of melanoma progression through AKT and ERK phosphorylation

Authors:
- Meng‑Yan Sun
- Yuchong Wang
- Ji Zhu
- Chuan Lv
- Kai Wu
- Xin‑Wei Wang
- Chun‑Yu Xue
View Affiliations / Copyright

Affiliations: Resident Standardized Training Center, Changzheng Hospital, The Second Military Medical University, Shanghai 200001, P.R. China, Department of Plastic Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China
Pages: 2673-2680
|
Published online on: March 30, 2018

https://doi.org/10.3892/or.2018.6341
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Abstract

Regulator of G‑protein signaling 1 (RGS1) has been found to be a critical factor in melanoma and other malignancies. However, the mechanism involved in the RGS1‑mediated promotion of melanoma progression is not clear. We based our study on samples collected from pathological specimens of melanoma patients. We found by immunohistochemistry that RGS1 expression was significantly higher in melanoma than that noted in nevus tissue (P<0.05). Kaplan‑Meier analysis demonstrated a significant correlation between increased RGS1 expression and reduced disease‑specific survival (P<0.05). RGS1 expression was also found to be related to the proliferation and migration of melanoma cells. RGS1 was able to bind to the Gαs in immunoprecipitation, but this interaction did not accelerate GTP hydrolysis in our experiment. Furthermore, we found that RGS1 may promote melanoma progression through the downstream effects of Gαs signaling, such as the increased phosphorylation of AKT and ERK by western blotting. Our results demonstrated that RGS1 promotes melanoma progression through regulation of Gαs‑mediated inactivation of AKT and ERK. Therefore, RGS1 is a novel therapeutic target for melanoma treatment.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Critical role for non‑GAP function of Gαs in RGS1‑mediated promotion of melanoma progression through AKT and ERK phosphorylation

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