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Article

miR-328-5p inhibits MDA-MB-231 breast cancer cell proliferation by targeting RAGE

  • Authors:
    • Tingting Luo
    • Yueqiong Yan
    • Qiuxia He
    • Xiaoqian Ma
    • Wei Wang
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasonography, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 2906-2914
    |
    Published online on: April 4, 2018
       https://doi.org/10.3892/or.2018.6353
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Abstract

MicroRNAs (miRNAs) are a class of short non-coding RNAs that play an important role in gene regulation and are critically involved in the pathogenesis and progression of human cancer. miR-328-5p has been reported to potentially act as a sensitising agent in breast cancer, but its other cellular functions and mechanisms remain unknown. The primary aim of the present study was to discover additional cellular functions and mechanisms of miR-328-5p in the breast cancer cell line MDA-MB-231. In the present study, miRNA microarray was used to find altered miRNAs. MTT and colony formation were used to test cell proliferation. Flow cytometry and western blotting were used to explore potential mechanisms of miR-328-5p regulating cell proliferation. A luciferase reporter assay was used to confirm target binding. miR-328-5p was revealed to be significantly upregulated after knockdown of the receptor for advanced glycosylation end products (RAGE). We also confirmed that miR-328-5p was frequently decreased in breast cancer tissues. Moreover, miR-328-5p mimics inhibited MDA-MB-231 proliferation, drug resistance and cell cycle progression. We confirmed that RAGE was a direct target of miR-328-5p. Functions of miR-328-5p in MDA-MB‑231 cells were elucidated by targeting RAGE. In conclusion, these results revealed that miR-328-5p may be considered as a tumour-suppressor factor, and promoting miR-328-5p expression could be a novel therapeutic strategy for breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Luo T, Yan Y, He Q, Ma X and Wang W: miR-328-5p inhibits MDA-MB-231 breast cancer cell proliferation by targeting RAGE. Oncol Rep 39: 2906-2914, 2018.
APA
Luo, T., Yan, Y., He, Q., Ma, X., & Wang, W. (2018). miR-328-5p inhibits MDA-MB-231 breast cancer cell proliferation by targeting RAGE. Oncology Reports, 39, 2906-2914. https://doi.org/10.3892/or.2018.6353
MLA
Luo, T., Yan, Y., He, Q., Ma, X., Wang, W."miR-328-5p inhibits MDA-MB-231 breast cancer cell proliferation by targeting RAGE". Oncology Reports 39.6 (2018): 2906-2914.
Chicago
Luo, T., Yan, Y., He, Q., Ma, X., Wang, W."miR-328-5p inhibits MDA-MB-231 breast cancer cell proliferation by targeting RAGE". Oncology Reports 39, no. 6 (2018): 2906-2914. https://doi.org/10.3892/or.2018.6353
Copy and paste a formatted citation
x
Spandidos Publications style
Luo T, Yan Y, He Q, Ma X and Wang W: miR-328-5p inhibits MDA-MB-231 breast cancer cell proliferation by targeting RAGE. Oncol Rep 39: 2906-2914, 2018.
APA
Luo, T., Yan, Y., He, Q., Ma, X., & Wang, W. (2018). miR-328-5p inhibits MDA-MB-231 breast cancer cell proliferation by targeting RAGE. Oncology Reports, 39, 2906-2914. https://doi.org/10.3892/or.2018.6353
MLA
Luo, T., Yan, Y., He, Q., Ma, X., Wang, W."miR-328-5p inhibits MDA-MB-231 breast cancer cell proliferation by targeting RAGE". Oncology Reports 39.6 (2018): 2906-2914.
Chicago
Luo, T., Yan, Y., He, Q., Ma, X., Wang, W."miR-328-5p inhibits MDA-MB-231 breast cancer cell proliferation by targeting RAGE". Oncology Reports 39, no. 6 (2018): 2906-2914. https://doi.org/10.3892/or.2018.6353
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