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Article

In vitro cytotoxic effect of PARP inhibitor alone and in combination with nab‑paclitaxel on triple‑negative and luminal A breast cancer cells

  • Authors:
    • Mehmet Topçul
    • İdi̇l Çeti̇n
    • Suna Özbaş Turan
    • Melek Özlem Kolusayin Ozar
  • View Affiliations / Copyright

    Affiliations: Department of Biology, Faculty of Science, Istanbul University, Vezneciler, Istanbul 34459, Turkey, Faculty of Pharmacy, Marmara University, Haydarpaşa, Istanbul 34668, Turkey, Department of Forensic Science, Cerrahpaşa Medical Faculty, Istanbul University, Fatih, Istanbul 34098, Turkey
  • Pages: 527-535
    |
    Published online on: April 12, 2018
       https://doi.org/10.3892/or.2018.6364
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Abstract

In the present study, the in vitro cytotoxic effect of poly(ADP‑ribose) polymerase (PARP) inhibitor alone and in combination with nab‑paclitaxel was evaluated on human triple‑negative breast cancer (TNBC) cell line MDA‑MB‑231 and human luminal A breast cancer cell line MCF‑7. For this purpose, cell index (CI) values obtained from xCELLigence Real‑Time Cell Analysis (RTCA) DP instrument, mitotic index (MI), labelling index (LI) and apoptotic index (AI) analysis among cell kinetic parameters were used. As a result of PARP inhibitor application, there was a significant decrease in CI, MI and LI and a significant increase in AI for all the experimental groups. After application of PARP inhibitor in combination with nab‑paclitaxel, the CI values were decreased for both cell lines, and the differences between the control and all the experimental groups were statistically significant (P<0.01) for all applications. PARP inhibitor, alone or in combination with nab‑paclitaxel offers a promising treatment modality in different breast cancer subtypes.
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Copy and paste a formatted citation
Spandidos Publications style
Topçul M, Çeti̇n İ, Özbaş Turan S and Kolusayin Ozar MÖ: In vitro cytotoxic effect of PARP inhibitor alone and in combination with nab‑paclitaxel on triple‑negative and luminal A breast cancer cells. Oncol Rep 40: 527-535, 2018.
APA
Topçul, M., Çeti̇n, İ., Özbaş Turan, S., & Kolusayin Ozar, M.Ö. (2018). In vitro cytotoxic effect of PARP inhibitor alone and in combination with nab‑paclitaxel on triple‑negative and luminal A breast cancer cells. Oncology Reports, 40, 527-535. https://doi.org/10.3892/or.2018.6364
MLA
Topçul, M., Çeti̇n, İ., Özbaş Turan, S., Kolusayin Ozar, M. Ö."In vitro cytotoxic effect of PARP inhibitor alone and in combination with nab‑paclitaxel on triple‑negative and luminal A breast cancer cells". Oncology Reports 40.1 (2018): 527-535.
Chicago
Topçul, M., Çeti̇n, İ., Özbaş Turan, S., Kolusayin Ozar, M. Ö."In vitro cytotoxic effect of PARP inhibitor alone and in combination with nab‑paclitaxel on triple‑negative and luminal A breast cancer cells". Oncology Reports 40, no. 1 (2018): 527-535. https://doi.org/10.3892/or.2018.6364
Copy and paste a formatted citation
x
Spandidos Publications style
Topçul M, Çeti̇n İ, Özbaş Turan S and Kolusayin Ozar MÖ: In vitro cytotoxic effect of PARP inhibitor alone and in combination with nab‑paclitaxel on triple‑negative and luminal A breast cancer cells. Oncol Rep 40: 527-535, 2018.
APA
Topçul, M., Çeti̇n, İ., Özbaş Turan, S., & Kolusayin Ozar, M.Ö. (2018). In vitro cytotoxic effect of PARP inhibitor alone and in combination with nab‑paclitaxel on triple‑negative and luminal A breast cancer cells. Oncology Reports, 40, 527-535. https://doi.org/10.3892/or.2018.6364
MLA
Topçul, M., Çeti̇n, İ., Özbaş Turan, S., Kolusayin Ozar, M. Ö."In vitro cytotoxic effect of PARP inhibitor alone and in combination with nab‑paclitaxel on triple‑negative and luminal A breast cancer cells". Oncology Reports 40.1 (2018): 527-535.
Chicago
Topçul, M., Çeti̇n, İ., Özbaş Turan, S., Kolusayin Ozar, M. Ö."In vitro cytotoxic effect of PARP inhibitor alone and in combination with nab‑paclitaxel on triple‑negative and luminal A breast cancer cells". Oncology Reports 40, no. 1 (2018): 527-535. https://doi.org/10.3892/or.2018.6364
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