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Microarray‑based bioinformatics analysis of the prospective target gene network of key miRNAs influenced by long non‑coding RNA PVT1 in HCC

  • Authors:
    • Yu Zhang
    • Wei‑Jia Mo
    • Xiao Wang
    • Tong‑Tong Zhang
    • Yuan Qin
    • Han‑Lin Wang
    • Gang Chen
    • Dan‑Ming Wei
    • Yi‑Wu Dang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250012, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 226-240
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    Published online on: May 2, 2018
       https://doi.org/10.3892/or.2018.6410
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Abstract

The long non‑coding RNA (lncRNA) PVT1 plays vital roles in the tumorigenesis and development of various types of cancer. However, the potential expression profiling, functions and pathways of PVT1 in HCC remain unknown. PVT1 was knocked down in SMMC‑7721 cells, and a miRNA microarray analysis was performed to detect the differentially expressed miRNAs. Twelve target prediction algorithms were used to predict the underlying targets of these differentially expressed miRNAs. Bioinformatics analysis was performed to explore the underlying functions, pathways and networks of the targeted genes. Furthermore, the relationship between PVT1 and the clinical parameters in HCC was confirmed based on the original data in the TCGA database. Among the differentially expressed miRNAs, the top two upregulated and downregulated miRNAs were selected for further analysis based on the false discovery rate (FDR), fold‑change (FC) and P‑values. Based on the TCGA database, PVT1 was obviously highly expressed in HCC, and a statistically higher PVT1 expression was found for sex (male), ethnicity (Asian) and pathological grade (G3+G4) compared to the control groups (P<0.05). Furthermore, Gene Ontology (GO) analysis revealed that the target genes were involved in complex cellular pathways, such as the macromolecule biosynthetic process, compound metabolic process, and transcription. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the MAPK and Wnt signaling pathways may be correlated with the regulation of the four candidate miRNAs. The results therefore provide significant information on the differentially expressed miRNAs associated with PVT1 in HCC, and we hypothesized that PVT1 may play vital roles in HCC by regulating different miRNAs or target gene expression (particularly MAPK8) via the MAPK or Wnt signaling pathways. Thus, further investigation of the molecular mechanism of PVT1 in HCC is needed.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Mo WJ, Wang X, Zhang TT, Qin Y, Wang HL, Chen G, Wei DM and Dang YW: Microarray‑based bioinformatics analysis of the prospective target gene network of key miRNAs influenced by long non‑coding RNA PVT1 in HCC. Oncol Rep 40: 226-240, 2018.
APA
Zhang, Y., Mo, W., Wang, X., Zhang, T., Qin, Y., Wang, H. ... Dang, Y. (2018). Microarray‑based bioinformatics analysis of the prospective target gene network of key miRNAs influenced by long non‑coding RNA PVT1 in HCC. Oncology Reports, 40, 226-240. https://doi.org/10.3892/or.2018.6410
MLA
Zhang, Y., Mo, W., Wang, X., Zhang, T., Qin, Y., Wang, H., Chen, G., Wei, D., Dang, Y."Microarray‑based bioinformatics analysis of the prospective target gene network of key miRNAs influenced by long non‑coding RNA PVT1 in HCC". Oncology Reports 40.1 (2018): 226-240.
Chicago
Zhang, Y., Mo, W., Wang, X., Zhang, T., Qin, Y., Wang, H., Chen, G., Wei, D., Dang, Y."Microarray‑based bioinformatics analysis of the prospective target gene network of key miRNAs influenced by long non‑coding RNA PVT1 in HCC". Oncology Reports 40, no. 1 (2018): 226-240. https://doi.org/10.3892/or.2018.6410
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Mo WJ, Wang X, Zhang TT, Qin Y, Wang HL, Chen G, Wei DM and Dang YW: Microarray‑based bioinformatics analysis of the prospective target gene network of key miRNAs influenced by long non‑coding RNA PVT1 in HCC. Oncol Rep 40: 226-240, 2018.
APA
Zhang, Y., Mo, W., Wang, X., Zhang, T., Qin, Y., Wang, H. ... Dang, Y. (2018). Microarray‑based bioinformatics analysis of the prospective target gene network of key miRNAs influenced by long non‑coding RNA PVT1 in HCC. Oncology Reports, 40, 226-240. https://doi.org/10.3892/or.2018.6410
MLA
Zhang, Y., Mo, W., Wang, X., Zhang, T., Qin, Y., Wang, H., Chen, G., Wei, D., Dang, Y."Microarray‑based bioinformatics analysis of the prospective target gene network of key miRNAs influenced by long non‑coding RNA PVT1 in HCC". Oncology Reports 40.1 (2018): 226-240.
Chicago
Zhang, Y., Mo, W., Wang, X., Zhang, T., Qin, Y., Wang, H., Chen, G., Wei, D., Dang, Y."Microarray‑based bioinformatics analysis of the prospective target gene network of key miRNAs influenced by long non‑coding RNA PVT1 in HCC". Oncology Reports 40, no. 1 (2018): 226-240. https://doi.org/10.3892/or.2018.6410
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