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Article

Elevated TFAP4 regulates lncRNA TRERNA1 to promote cell migration and invasion in gastric cancer

  • Authors:
    • Huazhang Wu
    • Xiufang Liu
    • Pihai Gong
    • Wei Song
    • Menghan Zhou
    • Yiping Li
    • Zhujiang Zhao
    • Hong Fan
  • View Affiliations / Copyright

    Affiliations: Department of Medical Genetics and Developmental Biology, Medical School of Southeast University and Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Pathology, Medical School of Southeast University, Nanjing, Jiangsu 210009, P.R. China
  • Pages: 923-931
    |
    Published online on: May 25, 2018
       https://doi.org/10.3892/or.2018.6466
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Abstract

Cancer cell invasion and metastasis are the leading causes of the high mortality rates in patients with malignant tumors. There is accumulating evidence to indicate that dysregulated long non‑coding RNAs (lncRNAs) may be involved in the progression of tumor invasion and metastasis. However, the regulatory mechanisms of the aberrant expression of lncRNAs remain largely unknown, although the roles of lncRNAs as drivers of tumor suppressive and oncogenic functions have appeared in prevalent cancer types in recent years. In the present study, we identified that the transcription factor, activating enhancer‑binding protein 4 (TFAP4), acts as a key modulator of translation regulatory long non‑coding RNA 1(TRERNA1), which has been proven to promote the invasion and metastasis of gastric cancer (GC) cells. We revealed that TRERNA1 was upregulated in gastric carcinogenesis and promoted cell migration and invasion in GC. Using bioinformatics analysis, we observed that there were several potential binding sites of TFAP4 in the promoter region of TRERNA1. The knockdown of TFAP4 significantly reduced the expression level of TRERNA1, whereas the ectopic expression of TFAP4 significantly increased the expression level of TRERNA1 in GC cell lines. Dual luciferase reporter assay combined with chromatin immunoprecipitation (ChIP) revealed that TFAP4 specifically regulated the transcriptional activity of TRERNA1 by binding to the E‑box motifs in the TRERNA1 promoter. In addition, there was a positive correlation between the TFAP4 and TRERNA1 expression level in clinical GC cases, which also indicated that TFAP4 can directly modulate the expression of TRERNA1. In the present study, we provide a novel potential therapeutic target and strategy for GC.
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Copy and paste a formatted citation
Spandidos Publications style
Wu H, Liu X, Gong P, Song W, Zhou M, Li Y, Zhao Z and Fan H: Elevated TFAP4 regulates lncRNA TRERNA1 to promote cell migration and invasion in gastric cancer. Oncol Rep 40: 923-931, 2018.
APA
Wu, H., Liu, X., Gong, P., Song, W., Zhou, M., Li, Y. ... Fan, H. (2018). Elevated TFAP4 regulates lncRNA TRERNA1 to promote cell migration and invasion in gastric cancer. Oncology Reports, 40, 923-931. https://doi.org/10.3892/or.2018.6466
MLA
Wu, H., Liu, X., Gong, P., Song, W., Zhou, M., Li, Y., Zhao, Z., Fan, H."Elevated TFAP4 regulates lncRNA TRERNA1 to promote cell migration and invasion in gastric cancer". Oncology Reports 40.2 (2018): 923-931.
Chicago
Wu, H., Liu, X., Gong, P., Song, W., Zhou, M., Li, Y., Zhao, Z., Fan, H."Elevated TFAP4 regulates lncRNA TRERNA1 to promote cell migration and invasion in gastric cancer". Oncology Reports 40, no. 2 (2018): 923-931. https://doi.org/10.3892/or.2018.6466
Copy and paste a formatted citation
x
Spandidos Publications style
Wu H, Liu X, Gong P, Song W, Zhou M, Li Y, Zhao Z and Fan H: Elevated TFAP4 regulates lncRNA TRERNA1 to promote cell migration and invasion in gastric cancer. Oncol Rep 40: 923-931, 2018.
APA
Wu, H., Liu, X., Gong, P., Song, W., Zhou, M., Li, Y. ... Fan, H. (2018). Elevated TFAP4 regulates lncRNA TRERNA1 to promote cell migration and invasion in gastric cancer. Oncology Reports, 40, 923-931. https://doi.org/10.3892/or.2018.6466
MLA
Wu, H., Liu, X., Gong, P., Song, W., Zhou, M., Li, Y., Zhao, Z., Fan, H."Elevated TFAP4 regulates lncRNA TRERNA1 to promote cell migration and invasion in gastric cancer". Oncology Reports 40.2 (2018): 923-931.
Chicago
Wu, H., Liu, X., Gong, P., Song, W., Zhou, M., Li, Y., Zhao, Z., Fan, H."Elevated TFAP4 regulates lncRNA TRERNA1 to promote cell migration and invasion in gastric cancer". Oncology Reports 40, no. 2 (2018): 923-931. https://doi.org/10.3892/or.2018.6466
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