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Article Open Access

Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma

  • Authors:
    • Ying Ji
    • Jinquan Jiang
    • Lihua Huang
    • Wei Feng
    • Zhang Zhang
    • Longyu Jin
    • Xiaowei Xing
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Center for Medical Experiments, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China
    Copyright: © Ji et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 783-792
    |
    Published online on: June 4, 2018
       https://doi.org/10.3892/or.2018.6473
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Abstract

Lung cancer is the most common cause of cancer‑related deaths, and early diagnosis and targeted therapy are extremely important in the treatment of this disease. Sperm‑associated antigen 4 (SPAG4) was recently found to be a novel cancer biomarker. In the present study, the expression of SPAG4 was revealed to be high in lung adenocarcinoma tissues as determined by western blotting and immunohistochemistry. SPAG4 knockdown by RNAi efficiently reduced the migration of the lung cancer A549 cells in vitro. Mechanistically, SPAG4 exerted its tumor promoting functions by interacting with Nesprin3 as determined by co‑immunoprecipitation (Co‑IP) and bimolecular fluorescence complementation (BiFC) techniques. In addition, immunofluorescence revealed that the level of SPAG4 in lung cancer cells could affect the location and expression of Nesprin3. Furthermore, silencing of Nesprin3 reduced the migration of A549 cells and we provided evidence to demonstrate that SPAG4 acted as a positive regulator of Nesprin3. The results revealed that SPAG4, in cooperation with Nesprin3, has a fundamental pathological function in the migration of lung carcinoma cells, and the SPAG4 gene may be useful for the clinical diagnosis and new treatment strategies in patients with lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Ji Y, Jiang J, Huang L, Feng W, Zhang Z, Jin L and Xing X: Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma. Oncol Rep 40: 783-792, 2018.
APA
Ji, Y., Jiang, J., Huang, L., Feng, W., Zhang, Z., Jin, L., & Xing, X. (2018). Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma. Oncology Reports, 40, 783-792. https://doi.org/10.3892/or.2018.6473
MLA
Ji, Y., Jiang, J., Huang, L., Feng, W., Zhang, Z., Jin, L., Xing, X."Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma". Oncology Reports 40.2 (2018): 783-792.
Chicago
Ji, Y., Jiang, J., Huang, L., Feng, W., Zhang, Z., Jin, L., Xing, X."Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma". Oncology Reports 40, no. 2 (2018): 783-792. https://doi.org/10.3892/or.2018.6473
Copy and paste a formatted citation
x
Spandidos Publications style
Ji Y, Jiang J, Huang L, Feng W, Zhang Z, Jin L and Xing X: Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma. Oncol Rep 40: 783-792, 2018.
APA
Ji, Y., Jiang, J., Huang, L., Feng, W., Zhang, Z., Jin, L., & Xing, X. (2018). Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma. Oncology Reports, 40, 783-792. https://doi.org/10.3892/or.2018.6473
MLA
Ji, Y., Jiang, J., Huang, L., Feng, W., Zhang, Z., Jin, L., Xing, X."Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma". Oncology Reports 40.2 (2018): 783-792.
Chicago
Ji, Y., Jiang, J., Huang, L., Feng, W., Zhang, Z., Jin, L., Xing, X."Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma". Oncology Reports 40, no. 2 (2018): 783-792. https://doi.org/10.3892/or.2018.6473
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