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August-2018 Volume 40 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Curcumin inhibits liver cancer by inhibiting DAMP molecule HSP70 and TLR4 signaling

  • Authors:
    • Biqiong Ren
    • Shudi Luo
    • Xuefei Tian
    • Zhichao Jiang
    • Guoying Zou
    • Fei Xu
    • Tieqiu Yin
    • Yiran Huang
    • Junlong Liu
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory of The Second People's Hospital of Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410007, P.R. China
  • Pages: 895-901
    |
    Published online on: June 11, 2018
       https://doi.org/10.3892/or.2018.6485
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Abstract

Curcumin has been revealed to inhibit liver cancer, however, no studies have reported that the mechanism of curcumin's action on liver cancer is related to damage-associated molecular pattern (DAMP) molecules heat shock protein 70 (HSP70) and the toll-like receptor 4 (TLR4) signaling. This study aimed to investigate whether the activation of TLR4 signaling by HSP70 could be inhibited by curcumin, thus investigating the possible mechanism of curcumin in the inhibition of liver cancer. Western blotting was used to evaluate the expression of the HSP70 and TLR4 in HepG2 cells and ELISA was used to detect the concentration of HSP70 in cell culture medium. A thermal tolerance HepG2 (HepG2TT) cell model was established to simulate HSP70 accumulation in the microenvironment. A certain concentration of curcumin was co-cultured with HepG2 and HepG2TT cells to observe the changes of HSP70 and TLR4. Our results revealed that heat stress significantly increased the expression of extracellular HSP70 (eHSP70) and TLR4 (P<0.01), but significantly reduced the expression of intracellular HSP70 (P<0.01). Curcumin inhibited proliferation, invasion, and metastasis of HepG2 cells, caused cells to remain in the DNA S phase, promoted apoptosis, and significantly reduced intracellular HSP70, eHSP70 and TLR4 levels of HepG2TT cells. Following the removal of curcumin, eHSP70 increased again. In summary, our results demonstrated that the antitumor effect of curcumin was related to the inhibition HSP70-TLR4 signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Ren B, Luo S, Tian X, Jiang Z, Zou G, Xu F, Yin T, Huang Y and Liu J: Curcumin inhibits liver cancer by inhibiting DAMP molecule HSP70 and TLR4 signaling. Oncol Rep 40: 895-901, 2018.
APA
Ren, B., Luo, S., Tian, X., Jiang, Z., Zou, G., Xu, F. ... Liu, J. (2018). Curcumin inhibits liver cancer by inhibiting DAMP molecule HSP70 and TLR4 signaling. Oncology Reports, 40, 895-901. https://doi.org/10.3892/or.2018.6485
MLA
Ren, B., Luo, S., Tian, X., Jiang, Z., Zou, G., Xu, F., Yin, T., Huang, Y., Liu, J."Curcumin inhibits liver cancer by inhibiting DAMP molecule HSP70 and TLR4 signaling". Oncology Reports 40.2 (2018): 895-901.
Chicago
Ren, B., Luo, S., Tian, X., Jiang, Z., Zou, G., Xu, F., Yin, T., Huang, Y., Liu, J."Curcumin inhibits liver cancer by inhibiting DAMP molecule HSP70 and TLR4 signaling". Oncology Reports 40, no. 2 (2018): 895-901. https://doi.org/10.3892/or.2018.6485
Copy and paste a formatted citation
x
Spandidos Publications style
Ren B, Luo S, Tian X, Jiang Z, Zou G, Xu F, Yin T, Huang Y and Liu J: Curcumin inhibits liver cancer by inhibiting DAMP molecule HSP70 and TLR4 signaling. Oncol Rep 40: 895-901, 2018.
APA
Ren, B., Luo, S., Tian, X., Jiang, Z., Zou, G., Xu, F. ... Liu, J. (2018). Curcumin inhibits liver cancer by inhibiting DAMP molecule HSP70 and TLR4 signaling. Oncology Reports, 40, 895-901. https://doi.org/10.3892/or.2018.6485
MLA
Ren, B., Luo, S., Tian, X., Jiang, Z., Zou, G., Xu, F., Yin, T., Huang, Y., Liu, J."Curcumin inhibits liver cancer by inhibiting DAMP molecule HSP70 and TLR4 signaling". Oncology Reports 40.2 (2018): 895-901.
Chicago
Ren, B., Luo, S., Tian, X., Jiang, Z., Zou, G., Xu, F., Yin, T., Huang, Y., Liu, J."Curcumin inhibits liver cancer by inhibiting DAMP molecule HSP70 and TLR4 signaling". Oncology Reports 40, no. 2 (2018): 895-901. https://doi.org/10.3892/or.2018.6485
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