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Article

Insulin-like growth factor-1 induces epithelial-mesenchymal transition in hepatocellular carcinoma by activating survivin

  • Authors:
    • Fangfeng Liu
    • Yongjie Sun
    • Bingqi Liu
    • Jun Lu
    • Hongguang Li
    • Huaqiang Zhu
    • Hengjun Gao
    • Xu Zhou
    • Hong Chang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China
  • Pages: 952-958
    |
    Published online on: June 20, 2018
       https://doi.org/10.3892/or.2018.6516
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Abstract

Insulin-like growth factor-1 (IGF-1), a small polypeptide hormone similar to insulin in protein structures, has been identified as an activator of epithelial-mesenchymal transition (EMT) pathways in several types of cancers. As a member of the inhibitor of apoptosis protein (IAP) family, survivin is implicated in the EMT of some cancers. However, the role of survivin on IGF-1-mediated EMT of hepatocellular carcinoma (HCC) has not been clarified. In the present study, we demonstrated that survivin was involved in the EMT process induced by IGF-1 in HCC cell line SMMC7721. With administration of different concentrations of IGF-1, survivin mRNA and protein expression were significantly increased and stimulated EMT in the tested cell line, while the increased invasive and migratory abilities of HCC cells and activation of the EMT process induced by IGF-1 were reversed after silencing of survivin expression by transfecting small interfering RNA. This was further confirmed by the observation of morphological changes, the decrease of invasive and migratory abilities and the downregulation of EMT markers, N-cadherin, vimentin and Snail, and the upregulation of E-cadherin. In conclusion, survivin may play a vital role in the IGF-1 signaling pathway by mediating EMT in HCC through the upregulation of the expression of EMT markers, and the knockdown of survivin expression may suppress the metastasis of HCC, which may provide new insights for the molecular therapy of HCC patients in clinical treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Liu F, Sun Y, Liu B, Lu J, Li H, Zhu H, Gao H, Zhou X and Chang H: Insulin-like growth factor-1 induces epithelial-mesenchymal transition in hepatocellular carcinoma by activating survivin. Oncol Rep 40: 952-958, 2018.
APA
Liu, F., Sun, Y., Liu, B., Lu, J., Li, H., Zhu, H. ... Chang, H. (2018). Insulin-like growth factor-1 induces epithelial-mesenchymal transition in hepatocellular carcinoma by activating survivin. Oncology Reports, 40, 952-958. https://doi.org/10.3892/or.2018.6516
MLA
Liu, F., Sun, Y., Liu, B., Lu, J., Li, H., Zhu, H., Gao, H., Zhou, X., Chang, H."Insulin-like growth factor-1 induces epithelial-mesenchymal transition in hepatocellular carcinoma by activating survivin". Oncology Reports 40.2 (2018): 952-958.
Chicago
Liu, F., Sun, Y., Liu, B., Lu, J., Li, H., Zhu, H., Gao, H., Zhou, X., Chang, H."Insulin-like growth factor-1 induces epithelial-mesenchymal transition in hepatocellular carcinoma by activating survivin". Oncology Reports 40, no. 2 (2018): 952-958. https://doi.org/10.3892/or.2018.6516
Copy and paste a formatted citation
x
Spandidos Publications style
Liu F, Sun Y, Liu B, Lu J, Li H, Zhu H, Gao H, Zhou X and Chang H: Insulin-like growth factor-1 induces epithelial-mesenchymal transition in hepatocellular carcinoma by activating survivin. Oncol Rep 40: 952-958, 2018.
APA
Liu, F., Sun, Y., Liu, B., Lu, J., Li, H., Zhu, H. ... Chang, H. (2018). Insulin-like growth factor-1 induces epithelial-mesenchymal transition in hepatocellular carcinoma by activating survivin. Oncology Reports, 40, 952-958. https://doi.org/10.3892/or.2018.6516
MLA
Liu, F., Sun, Y., Liu, B., Lu, J., Li, H., Zhu, H., Gao, H., Zhou, X., Chang, H."Insulin-like growth factor-1 induces epithelial-mesenchymal transition in hepatocellular carcinoma by activating survivin". Oncology Reports 40.2 (2018): 952-958.
Chicago
Liu, F., Sun, Y., Liu, B., Lu, J., Li, H., Zhu, H., Gao, H., Zhou, X., Chang, H."Insulin-like growth factor-1 induces epithelial-mesenchymal transition in hepatocellular carcinoma by activating survivin". Oncology Reports 40, no. 2 (2018): 952-958. https://doi.org/10.3892/or.2018.6516
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