Open Access

miR‑195‑5p regulates multi‑drug resistance of gastric cancer cells via targeting ZNF139

  • Authors:
    • Hongfeng Nie
    • Jixing Mu
    • Jinsheng Wang
    • Yong Li
  • View Affiliations

  • Published online on: June 25, 2018     https://doi.org/10.3892/or.2018.6524
  • Pages: 1370-1378
  • Copyright: © Nie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer (GC) is one of the most common malignant tumors with a high mortality rate. Reversing the multi‑drug resistance (MDR) of GC offers the potential for significant enhancement of the effect of chemotherapy and improvement of prognosis. Aberrant microRNA expression can attribute to the pathogenesis of GC. However, the effects of microRNA (miR)‑195‑5p on the MDR of GC cells remains to be fully elucidated. In the present study, the effect of miR‑195‑5p in regulating the MDR of GC cells was investigated. Reverse transcription quantitative‑polymerase chain reaction was used to analyze the levels of miR‑195‑5p in GC cells. Western blot analysis was performed to analyze the protein levels of ZNF139, P‑gp, BCL‑2 and MRP1. The chemosensitivity of GC cells was determined by MTT. The results showed that the expression of miR‑195‑5p was decreased in poorly differentiated GC tissues with a higher chemosensitivity. The overexpression of miR‑195‑5p promoted the chemosensitivity of GC cells. Bioinformatics analysis indicated that Zing finger 139 (ZNF139) was a target of miR‑195‑5p. miR‑195‑5p negatively regulated the expression of ZNF139 by binding to its 3'‑untranslated region. The silencing of ZNF139 promoted the chemosensitivity of GC cells, and the downregulation of ZNF139 reversed the effect of miR‑195‑5p inhibitor on the chemosensitivity of GC cells. In conclusion, miR‑195‑5p regulated the MDR of GC cells via targeting ZNF139.
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September-2018
Volume 40 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Nie H, Mu J, Wang J and Li Y: miR‑195‑5p regulates multi‑drug resistance of gastric cancer cells via targeting ZNF139. Oncol Rep 40: 1370-1378, 2018
APA
Nie, H., Mu, J., Wang, J., & Li, Y. (2018). miR‑195‑5p regulates multi‑drug resistance of gastric cancer cells via targeting ZNF139. Oncology Reports, 40, 1370-1378. https://doi.org/10.3892/or.2018.6524
MLA
Nie, H., Mu, J., Wang, J., Li, Y."miR‑195‑5p regulates multi‑drug resistance of gastric cancer cells via targeting ZNF139". Oncology Reports 40.3 (2018): 1370-1378.
Chicago
Nie, H., Mu, J., Wang, J., Li, Y."miR‑195‑5p regulates multi‑drug resistance of gastric cancer cells via targeting ZNF139". Oncology Reports 40, no. 3 (2018): 1370-1378. https://doi.org/10.3892/or.2018.6524